Results 11 to 20 of about 244,648 (302)

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi, Aebersold, Aivaliotis, Aivaliotis, Alla, Ames, Babu, Bagshaw, Bell, Berlanda-Scorza, Berven, Bierczynska-Krzysik, Bisle, Bjellqvist, Bunai, Bunai, Burré, Buxbaum, Casey, Chevallet, Chevallet, Connolly, Coughenour, Cutillas, Devreese, Eravci, Everberg, Felsted, Ferro, Gatlin, Ghosh, Gyenes, Görg, Hagan, Hahne, Hartinger, Helling, Henningsen, Henzel, Hisabori, Hjelmeland, Huang, Huang, James, Jun, Lasonder, Lescuyer, Liberatori, Lopez, Low, Luche, Lyubimova, Lüddens, MacFarlane, MacFarlane, Matsudaira, McDonough, Miguet, Moebius, Molloy, Molloy, Nandakumar, Natera, Navarre, Ong, Papasotiriou, Pappin, Patton, Pedersen, Peng, Perdew, Phadke, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rais, Rubin, Santoni, Santoni, Schindler, Schirle, Schägger, Siroy, Speers, Sweetnam, Switzer, Tastet, Taylor, Towbin, Veith, Washburn, Werner, Wilkins, Williams, Williams, Williams, Wittig, Witzmann, Wu, Yates, Ying, Zahedi   +103 more
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Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]

, 1982
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő, Dudás, Brigitta, Erdei, Sára, Orosz, László   +3 more
core   +1 more source

A semi-virtual two dimensional gel electrophoresis: IF–ESI LC-MS/MS

MethodsX, 2017
A method for increasing the productivity of ESI LC-MS/MS (electrospray ionization-liquid chromatography-tandem mass spectrometry) was proposed and applied. After IF (isoelectric focusing) of the sample using IPG (immobilized pH gradient) strip, the strip
Stanislav Naryzhny, Victor Zgoda, Artur Kopylov, Elena Petrenko, Аlexander Archakov   +4 more
doaj   +1 more source

Mitochondrial proteomics: analysis of a whole mitochondrial extract with two-dimensional electrophoresis [PDF]

, 2008
Mitochondria are complex organelles, and their proteomics analysis requires a combination of techniques. The emphasis in this chapter is made first on mitochondria preparation from cultured mammalian cells, then on the separation of the mitochondrial ...
A. Gorg, C. Tastet, D. Mokranjac, F. Gharahdaghi, F. Granier, H. Zischka, J. C. Sanchez, M. Chevallet, M. Eschenbruch, P. Lescuyer, S. Iwata, S. Luche, S. Luche, S. Richert, T. Rabilloud, T. Tsukihara, V. Santoni   +16 more
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Advances in two-dimensional electrophoresis [PDF]

Nature, 1989
New developments in the first dimension step of two-dimensional electrophoresis have expanded the utility of the technique in cell and molecular biology.
Hanash, Samir M., Strahler, John R.
openaire   +3 more sources

Analysis of proteins from marine molluscs [PDF]

, 2009
Application of the two-dimensional gel electrophoresis (2DE) protocols which were developed for samples of mammalian origin gives unsatisfactory results when used in samples from marine molluscs. This chapter describes a detailed protocol of 2DE that can
Bebianno, Maria João, Chora, Suze, Roméo, Michèle   +2 more
core   +1 more source

Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update [PDF]

, 2008
The quality and ease of proteomics analysis depends on the performance of the analytical tools used, and thus of the performances of the protein separation tools used to deconvolute complex protein samples.
Adessi, Aivaliotis, Aivaliotis, Alla, Ames, Babu, Berlanda Scorza, Berven, Bierczynska-Krzysik, Bisle, Braun, Bunai, Bunai, Burré, Burré, Buxbaum, Carboni, Chevallet, Chevallet, Connolly, Coughenour, Englund, Everberg, Felsted, Ferro, Friso, Galeva, Gatlin, Gianazza, Gyenes, Hagan, Hartinger, Helling, Henningsen, Hjelmeland, Huang, Huang, Jun, Klein, Lescuyer, Liberatori, Lopez, Low, Luche, Lyubimova, Lüddens, MacFarlane, MacFarlane, Mastro, McDonough, Moebius, Molloy, Molloy, Molloy, Mócz, Nandakumar, Navarre, Patton, Paulson, Pedersen, Peirce, Peng, Perdew, Phadke, Quaranta, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rais, Richert, Rubin, Santoni, Santoni, Santoni, Schindler, Schägger, Siroy, Speers, Stanley, Sweetnam, Tastet, Taylor, Unlü, Valot, Veith, Watarai, Werhahn, Wilkins, Williams, Williams, Wittig, Witzmann, Ying, Zahedi   +95 more
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Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells [PDF]

, 1978
The structure of the 5'-termini has been investigated in mitochondrial DNA- coded poly(A)-containing RNA from HeLa cells. For this purpose, mitochondrial RNA isolated from cells labeled for 3 hours with [32P]orthophosphate in the presence of 20 µg/ml ...
Amalric, François, Attardi, Giuseppe, Crews, Stephen, Grohmann, Karel   +3 more
core   +1 more source

Comparative two-dimensional gel analysis and microsequencing identifies gelsolin as one of the most prominent downregulated markers of transformed human fibroblast and epithelial cells [PDF]

, 1990
A systematic comparison of the protein synthesis patterns of cultured normal and transformed human fibroblasts and epithelial cells, using two-dimensional gel protein analysis combined with computerized imaging and data acquisition, identified a 90-kD ...
Bauw, Guy, Celis, Julio E, Honoré, Bent, Vancompernolle, Katia, Vandekerckhove, Joël   +4 more
core   +1 more source

Alpha1-acid glycoprotein post-translational modifications: a comparative two dimensional electrophoresis based analysis

Italian Journal of Animal Science, 2010
Alpha1-acid glycoprotein (AGP) is an immunomodulatory protein expressed by hepatocytes in response to the systemic reaction that follows tissue damage caused by inflammation, infection or trauma.
P. Roncada, G. F. Greppi, G. Candiano, L. Musante, A. Miranda-Ribeira, P. Sartorelli, F. Ceciliani, R. Fortin   +7 more
doaj   +1 more source