Results 41 to 50 of about 115,445 (261)

Towards the Full Realization of 2DE Power

Proteomes, 2016
Here, approaches that allow disclosure of the information hidden inside and outside of two-dimensional gel electrophoresis (2DE) are described. Experimental identification methods, such as mass spectrometry of high resolution and sensitivity (MALDI-TOF ...
Stanislav Naryzhny
doaj   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Proteome Analysis of the Plant Pathogenic Fungus Monilinia laxa Showing Host Specificity

Food Technology and Biotechnology, 2012
Brown rot fungus Monilinia laxa (Aderh. & Ruhl.) Honey is an important plant pathogen in stone and pome fruits in Europe. We applied a proteomic approach in a study of M. laxa isolates obtained from apples and apricots in order to show the host specifity
Olja Bregar, Stanislav Mandelc, Franci Celar, Branka Javornik   +3 more
doaj  

Changes in the proteomic profile of blood serum in coronary atherosclerosis [PDF]

Journal of Medical Biochemistry, 2020
Background: Our aim was to study changes in the serum proteomic profile in coronary atherosclerosis. Methods: The study involved two groups of patients: 1) men with coronary heart disease and coronary atherosclerosis (n = 15); 2) control (n = 15): men ...
Stakhneva Ekaterina M., Meshcheryakova Irina A., Demidov Evgeny A., Starostin Konstantin V., Peltek Sergey E., Voevoda Michael I., Ragino Yuliya I.   +6 more
doaj  

Hierarchical transformation-guided image registration for two-dimensional gel electrophoresis image

Journal of Algorithms & Computational Technology, 2020
Many proteomics studies have been devoted to investigate the gel-based methods for separation and fractionation of complex protein mixture extracted from tissues, cells, and other biological specimens.
Lifang Wei, Heng Dong, Changcai Yang, Zhe Guan, Wenfang Lin, Ying Miao   +5 more
doaj   +1 more source

Comparative Two-Dimensional Fluorescence Gel Electrophoresis [PDF]

, 2017
Abstract Comparative two-dimensional gel electrophoresis (CoFGE) is a special version of two-dimensional polyacrylamide GE (2D-PAGE) and related to difference GE (2D-DIGE). It provides reproducibility and standardisation for 2D-PAGE by introducing a reference to the experiment.
Simone König, Doreen Ackermann
openaire   +4 more sources

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

The Response of Rice Root to Time Course Water Deficit Stress-Two Dimensional Electrophoresis Approach [PDF]

Ikufīziyuluzhī-i Giyāhān-i Zirā̒ī, 2015
Rice (Oryza sativa L.) is the staple food of more than half of the population worldwide. Water deficit stress is one of the harsh limiting factors for successful production of crops.
Mahmood Toorchi
doaj  

Redox options in two-dimensional electrophoresis

Amino Acids, 2005
Two-dimensional electrophoresis is usually run on fully reduced samples. Under these conditions even covalently bound oligomers are dissociated and individual polypeptide chains may be fully unfolded by both, urea and SDS, which maximizes the number of resolved components and allows their pI and M(r) to be most accurately evaluated.
R. Wait, S. Begum, D. Brambilla, A. M. Carabelli, F. Conserva, A. Guerini Rocco, I. Eberini, R. Ballerio, M. Gemeiner, I. Miller, E. Gianazza   +10 more
openaire   +4 more sources

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source