Results 161 to 170 of about 209,274 (278)

Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans. [PDF]

Heliyon
Maar S, Czuni L, Hassve JK, Takatsy A, Rendeki S, Mintal T, Gallyas F, Bock-Marquette I.   +7 more
europepmc   +1 more source

Optimization of protein extraction and two-dimensional gel electrophoresis profiles for the identification of Cordyceps sinensis and other similar species. [PDF]

PLoS One, 2018
Chan RCH, Lam SSW, Fong FLY, Chan DTW, Lee FWF, Sze ETP.   +5 more
europepmc   +1 more source

VE‐Cadherin–Actin Regulation Promotes Mechanotransduction and Monolayer Maturation Involving a Tension‐Sensitive Intermediate State

Advanced Science, EarlyView.
This study investigates how endothelial monolayers balance cohesion and plasticity during mechanical stimulation. In HUVECs, shear stress induces a tension‐sensitive intermediate state in which junctional VE‐cadherin and actomyosin relaxation levels coordinate actin/integrin remodeling. This rapid, reversible state strengthens cell junctions, preserves
Jonas Franz, Maria Odenthal‐Schnittler, Jan Philip Kipcke, Jochen Seebach, Zahra Labbaf, Franziska Merten, Vesna Bojovic, Muna Taha, Johannes A. Eble, Erez Raz, Milos Galic, Hans Schnittler   +11 more
wiley   +1 more source

Improved methods for total and chloroplast protein extraction from Cajanus species for two-dimensional gel electrophoresis and mass spectrometry. [PDF]

PLoS One
S A, Das A, Kalita PJ, Patil RI, Pandey N, Bhattacharjee M, Sharma BK, Das D, Acharjee S.   +8 more
europepmc   +1 more source

Detection of plant protein adulterated in fluid milk using two-dimensional gel electrophoresis combined with mass spectrometry. [PDF]

J Food Sci Technol, 2018
Yang J, Zheng N, Yang Y, Wang J, Soyeurt H.   +4 more
europepmc   +1 more source

Structure‐Guided Engineering of a Cas12i Nuclease Unlocks Near‐PAMless Genome Editing

Advanced Science, EarlyView.
CRISPR‐Cas nucleases are limited by PAM requirements, restricting genome accessibility. Structure‐guided engineering of the compact Cas12i nuclease SF01 produced three variants with near‐PAMless, enabling efficient editing at diverse 5'‐NNTN‐3' sites. These nucleases expand the editable portion of the human genome more than fourfold, enabling efficient
Qitong Chen, Hanlin Gou, Chao Xu, Sihan Wang, Huitao Zhang, Minglei Song, Mengge Wang, Xingkun Ji, Xiaofei Wei, Yuanyan Tan, Hehua Quan, Pengyu Luo, Hanyu Shou, Pengpeng Liu, Yafeng Liang, Jian‐Kang Zhu   +15 more
wiley   +1 more source

Promotion of DFU Wound Healing via BRG1–COL16A1 Axis in Fibroblasts

Advanced Science, EarlyView.
In normal wound healing, transcription factor BRG1 is upregulated and binds the COL16A1 promoter to enhance its expression, promoting fibroblast proliferation, migration, contraction, extracellular matrix deposition, and granulation tissue formation, thus accelerating wound closure.
Penghui Wang, Jiaming Sun, Ying Wang, Bichen Ren, Yuxin Liu, Yunhan Liu, Liang Chen, Lu Yu, Tao Dai, Li Yu, Zhihui Dong   +10 more
wiley   +1 more source

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome. [PDF]

J Vis Exp, 2018
Zhan X, Huang Y, Long Y.
europepmc   +1 more source

Spatially Resolved Multiomics Reveals Metabolic Remodeling and Autophagy Activation in Adamantinomatous Craniopharyngiomas

Advanced Science, EarlyView.
Multiomics integration analysis reveals the “cystic fluid–tumor cell” metabolic coupling that mediates active choline/ethanolamine uptake of tumor cells from cystic fluid and PC/PE synthesis pathways reprogramming that mediating autophagy pathway activation within ACP. ABSTRACT Adamantinomatous craniopharyngioma (ACP), a benign yet highly recurrent and
Dongting Chen, Yahui Gao, Yulin Wang, Ting Lei, Zheng Qu, Yuhan An, Jiaxu Fu, Xin Li, Fangjun Liu, Yan Li   +9 more
wiley   +1 more source

Screening of differentially expressed proteins in psoriasis vulgaris by two-dimensional gel electrophoresis and mass spectrometry. [PDF]

Exp Ther Med, 2017
Dai Y, Zhang Q, Jiang Y, Yin L, Zhang X, Chen Y, Cai X.   +6 more
europepmc   +1 more source