Results 251 to 260 of about 209,274 (278)

Two-dimensional gel electrophoresis of proteins

Journal of Chromatography B: Biomedical Sciences and Applications, 1987
The high-resolution capacity of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) makes it an excellent tool for the analysis and characterisation of complex protein mixtures. The evolution of two-dimensional electrophoresis is briefly described.
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High Resolution Two-dimensional Polyacrylamide-gel Electrophoresis

TrAC Trends in Analytical Chemistry, 1983
Abstract The combination of two different gel electrophoretic techniques in a two-dimensional separation procedure provides the resolution capacity required for the simultaneous separation and analysis of complex protein mixtures. This technique is therefore a powerful tool for the study of phenotypic expression.
Michael J. Dunn, Arthur H.M. Burghes
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Two‐Dimensional Blue Native Polyacrylamide Gel Electrophoresis

Current Protocols in Cell Biology, 2008
AbstractMultiprotein complexes play crucial roles in nearly all cell biological processes. Blue Native Polyacrylamide Gel Electrophoresis (BN‐PAGE) is a powerful method to study these complexes. It is a native protein separation method that relies on the dye Coomassie blue to confer negative charge for separation.
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Two-dimensional agarose gel electrophoresis without gel manipulation

Analytical Biochemistry, 1985
The apparatus and procedure to perform two-dimensional agarose gel electrophoresis without manipulating the gel used for the first electrophoresis (first-dimension gel) have been developed. The procedure is less complex, less damaging to first-dimension gels, and more precise than procedures that require manipulation of the first-dimension gel.
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Two-dimensional gel electrophoresis of membrane proteins

Biochemistry, 1976
A high-resolution method for two-dimensional separation of membrane proteins is described. It involves a nondiscriminating solubilization of a membrane preparation with sodium dodecyl sulfate, followed by electrophoresis in the first dimension according to charge (by isoelectric focusing).
G F, Ames, K, Nikaido
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Sample preparation for two‐dimensional gel electrophoresis

PROTEOMICS, 2003
Abstract The choice of sample preparation protocol is a critical influential factor for isoelectric focusing which in turn affects the two‐dimensional gel result in terms of quality and protein species distribution. The optimal protocol varies depending on the nature of the sample for analysis and the properties of the constituent ...
Margaret M, Shaw, Beat M, Riederer
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6 Two-dimensional gel electrophoresis

2005
Summary 2-DE continues to set the benchmark as the premier technique for protein separation in protoemic applications. The approach is very well sited for routine separation of moderately abundant proteins within a window of pH 3-10 and M r =10-200 kDa, allowing for cataloging and quantitative measures of protein expression.
Mark P. Molloy, Michael T. McDowell
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Two-Dimensional Gel Electrophoresis: A Reference Protocol

2017
Two-dimensional gel electrophoresis (2DE) has been a mainstay of proteomic techniques for more than four decades. It was even in use for several years before the term proteomics was actually coined in the early 1990s. Over this time, it has been used in the study of many diseases including cancer, diabetes, heart disease, and psychiatric disorders ...
Veronica M, Saia-Cereda, Adriano, Aquino, Paul C, Guest, Daniel, Martins-de-Souza   +3 more
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Two-dimensional Gel Electrophoresis (2DE)

2013
The chemical compounds, which are present in the environment, increasingly cause bad effects on health. The most serious effects are tumors and various mutations at the cellular level. Such compounds, from the analytical point of view, can serve the function of biomarkers, constituting measurable changes in the organism’s cells and biochemical ...
Ewa Kłodzińska, Bogusław Buszewski
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Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics

2016
Phosphorylation is one of the most important reversible protein modifications and is involved in regulating signal transduction, subcellular localization and enzyme activity of target proteins. Phosphorylation or dephosphorylation of proteins is directly reflected in changed ratios of phosphoprotein abundance and total protein abundance.
Chao, Han, Pingfang, Yang
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