Results 271 to 280 of about 533,494 (308)

Application of Brønsted-Type LFER in the Study of the Phospholipase C Mechanism

Journal of the American Chemical Society, 2003
Phosphatidylinositol-specific phospholipase C cleaves the phosphodiester bond of phosphatidylinositol to form inositol 1,2-cyclic phosphate and diacylglycerol. This enzyme also accepts a variety of alkyl and aryl inositol phosphates as substrates, making it a suitable model enzyme for studying mechanism of phosphoryl transfer by probing the linear free-
Karol S. Bruzik, Ming-Daw Tsai, Alexander V. Kravchuk, Cornelia Mihai   +3 more
openaire   +3 more sources

Phosphatidyl inositol-specific phospholipase C from Clostridium novyi type A

Archives of Biochemistry and Biophysics, 1978
Abstract From the culture broth of Clostridium novyi type A, phosphatidyl inositol-specific phospholipase C was separated from the major part of phospholipase C (γ-toxin) which hydrolyzes phosphatidyl choline, phosphatidyl ethanolamine, and sphingomyelin. Sodium deoxycholate stimulated the activity of phosphatidyl inositol phospholipase C.
Hiroh Ikezawa, Ryo Taguchi
openaire   +3 more sources

Tissue- and cell type-specific expression of mRNAS for four types of inositol phospholipid-specific phospholipase C

Biochemical and Biophysical Research Communications, 1989
The mRNA levels for four types of inositol phospholipid-specific phospholipase C (PLC) in various tissues and cell cultures have been studied by Northern analysis using cDNA probes for PLC isozyme I, II, and III [Sue, P.-G., Ryu, S.H., Moon, K.H., Sue, H.W., and Rhee, S.G. (1988) Proc. Natl. Acad. Sci.
Hiroyuki Sorimachi, Tadaomi Takenawa, Yoshimi Homma, Koichi Suzuki, Yasufumi Emori   +4 more
openaire   +3 more sources

The Small GTPase Ral Couples the Angiotensin II Type 1 Receptor to the Activation of Phospholipase C-δ1 [PDF]

Molecular Pharmacology, 2010
The angiotensin II type 1 receptor (AT(1)R) plays an important role in cardiovascular function and as such represents a primary target for therapeutic intervention. The AT(1)R has traditionally been considered to be coupled to the activation of phospholipase C (PLC) beta via its association with G alpha(q/11), leading to increases in intracellular ...
Stephen S. G. Ferguson, Sean P. Cregan, Lianne B. Dale, Lucimar T. Ferreira, Christina M. Godin, Robert Gros   +5 more
openaire   +2 more sources

Purification of recombinant SH2SH3 proteins of phospholipase C-γ1 and -γ2 and their inhibitory effect on PIP2-hydrolysis induced by both types of phospholipase C-γ

Biochemical and Biophysical Research Communications, 1992
In order to examine physiological function of the SH2/SH3 region of phospholipase C-gamma (Z region), we independently expressed cDNA fragments corresponding to the SH2/SH3 region of PLC-gamma 1 and PLC-gamma 2 in Escherichia coli. Although these recombinant proteins were recovered in particulate fractions by centrifugation of cell extracts, they were ...
Tadaomi Takenawa, Yoshimi Homma, Yasufumi Emori   +2 more
openaire   +2 more sources

Studies on phosphatidylinositol phosphodiesterase (phospholipase C type) of Bacillus cereus

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976
A phosphatidylinositol phosphodiesterase from the culture broth of Bacillus cereus, was purified to a homogeneous state as indicated by polyacrylamide gel electrophoresis, by ammonium sulfate precipitation and chromatography with DEAE-cellulose and CM-Sephadex.
Miyashita Tomoyuki, Ikezawa Hiroh, Yamanegi Masato, Taguchi Ryo, Ohyabu Tetsuo   +4 more
openaire   +3 more sources

Purification and characterization of a cytosolic phosphoinositide-phospholipase C (γ2-type) from human platelets

Biochemical and Biophysical Research Communications, 1990
A human platelet cytosolic phosphoinositide-specific phospholipase C, one of four PLC activity peaks separated by column chromatographies, designated as cPLC-I, was purified to homogeneity. The cPLC-I exhibited an apparent Mr of 145 kDa by SDS-polyacrylamide gel electrophoresis and was immunologically identified to be PLC-gamma 2.
Yoshinori Nozawa, Toshihiko Nakashima, Aiming Yu, Tadaomi Takenawa, Yoshimi Homma, Yoshiko Banno   +5 more
openaire   +2 more sources

Effects of selective phosphodiesterase type IV inhibitor, rolipram, on signal transducing phospholipases in neutrophil: Inhibition of phospholipases A2, D but not C

Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 1995
The effects of a selective phosphodiesterase (PDE) type IV inhibitor, rolipram, on activation of neutrophil phospholipases in response to the chemotactic peptide formyl-methiony-leucyl-phenylalanine (fMLP) were investigated. fMLP caused liberation of arachidonic acid, a precursor of eicosanoids and in the presence of 0.3% butanol, production of ...
Yoshihiro Katagiri, Toshihiro Mizutani, Yoshinori Nozawa, Yoshikatsu Nakamura, Mitsuhiro Nakamura, Seiko O. Takemura, Shigeru Nakashima, Hideo Miyata   +7 more
openaire   +3 more sources

The Promoter Activity of the Phospholipase C-γ2 Gene Is Regulated by a Cell-Type-Specific Control Element

DNA and Cell Biology, 1997
We have cloned and characterized a genomic DNA spanning the 5'-flanking region, the first and second exons, and the first intron of the human PLC-gamma2 gene. The proximal upstream region is highly GC-rich and lacks a TATA box, whereas the distal region contains several AT-rich tracts.
Lee, SJ, Bahk, YY, Yun, DH, Lee, HJ, Lee, YH, Ryu, SH, Suh, PG   +6 more
openaire   +4 more sources

Studies on phosphatidylinositol phosphodiesterase (phospholipase C type) of Bacillus cereus

Archives of Biochemistry and Biophysics, 1978
Abstract A phosphatidylinositol-specific phospholipase C (PIase), purified 447-fold from the culture broth of Bacillus cereus by ammonium sulfate precipitation and chromatography with CM-Sephadex and DEAE-cellulose, was analyzed in vitro and in vivo for its activity to induce the release of alkaline phosphatase from plasma membrane.
Tetsuo Ohyabu, Hiroh Ikezawa, Ryo Taguchi   +2 more
openaire   +3 more sources