Results 291 to 300 of about 32,298 (336)

A dual component system instructs membrane hydrolysis during the final stages of plant autophagy

open access: yes
Castets J   +15 more
europepmc   +1 more source
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Phospholipase c from Clostridium novyi type a. I

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
1. Phospholipase C (EC 3.1.4.3) from Clostridium novyi (oedematiens) type A was purified 2000-fold by (NH4)2SO4 precipitation, DEAE-Sephadex treatment in a batchwise system and Sephadex G-100 column chromatography. 2. The purified preparation had a specific activity of 95 mumol per min per mg protein toward phosphatidylcholine.
R, Taguchi, H, Ikezawa
openaire   +2 more sources

Phosphatidyl inositol-specific phospholipase C from Clostridium novyi type A

Archives of Biochemistry and Biophysics, 1978
Abstract From the culture broth of Clostridium novyi type A, phosphatidyl inositol-specific phospholipase C was separated from the major part of phospholipase C (γ-toxin) which hydrolyzes phosphatidyl choline, phosphatidyl ethanolamine, and sphingomyelin. Sodium deoxycholate stimulated the activity of phosphatidyl inositol phospholipase C.
R, Taguchi, H, Ikezawa
openaire   +2 more sources

Application of Brønsted-Type LFER in the Study of the Phospholipase C Mechanism

Journal of the American Chemical Society, 2003
Phosphatidylinositol-specific phospholipase C cleaves the phosphodiester bond of phosphatidylinositol to form inositol 1,2-cyclic phosphate and diacylglycerol. This enzyme also accepts a variety of alkyl and aryl inositol phosphates as substrates, making it a suitable model enzyme for studying mechanism of phosphoryl transfer by probing the linear free-
Cornelia, Mihai   +3 more
openaire   +2 more sources

Tissue- and cell type-specific expression of mRNAS for four types of inositol phospholipid-specific phospholipase C

Biochemical and Biophysical Research Communications, 1989
The mRNA levels for four types of inositol phospholipid-specific phospholipase C (PLC) in various tissues and cell cultures have been studied by Northern analysis using cDNA probes for PLC isozyme I, II, and III [Sue, P.-G., Ryu, S.H., Moon, K.H., Sue, H.W., and Rhee, S.G. (1988) Proc. Natl. Acad. Sci.
Y, Homma   +4 more
openaire   +2 more sources

Purification and characterization of a cytosolic phosphoinositide-phospholipase C (γ2-type) from human platelets

Biochemical and Biophysical Research Communications, 1990
A human platelet cytosolic phosphoinositide-specific phospholipase C, one of four PLC activity peaks separated by column chromatographies, designated as cPLC-I, was purified to homogeneity. The cPLC-I exhibited an apparent Mr of 145 kDa by SDS-polyacrylamide gel electrophoresis and was immunologically identified to be PLC-gamma 2.
Y, Banno   +5 more
openaire   +2 more sources

A Bacterial-Toxin Type of Phospholipase (Lecithinase C) in a Marine Phytoplanktonic Chrysomonad

Journal of the Fisheries Research Board of Canada, 1967
not available
N. J. Antia, E. Bilinski
openaire   +1 more source

Effects of selective phosphodiesterase type IV inhibitor, rolipram, on signal transducing phospholipases in neutrophil: Inhibition of phospholipases A2, D but not C

Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 1995
The effects of a selective phosphodiesterase (PDE) type IV inhibitor, rolipram, on activation of neutrophil phospholipases in response to the chemotactic peptide formyl-methiony-leucyl-phenylalanine (fMLP) were investigated. fMLP caused liberation of arachidonic acid, a precursor of eicosanoids and in the presence of 0.3% butanol, production of ...
S, Nakashima   +7 more
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Purification of recombinant SH2SH3 proteins of phospholipase C-γ1 and -γ2 and their inhibitory effect on PIP2-hydrolysis induced by both types of phospholipase C-γ

Biochemical and Biophysical Research Communications, 1992
In order to examine physiological function of the SH2/SH3 region of phospholipase C-gamma (Z region), we independently expressed cDNA fragments corresponding to the SH2/SH3 region of PLC-gamma 1 and PLC-gamma 2 in Escherichia coli. Although these recombinant proteins were recovered in particulate fractions by centrifugation of cell extracts, they were ...
Y, Homma, Y, Emori, T, Takenawa
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Humoral and Delayed-type Hypersensitive Responses againstListeria monocytogenes Phosphatidylinositol-specific Phospholipase C in Experimentally Infected Buffaloes

Veterinary Research Communications, 2004
The kinetics of antibody production against phosphatidylinositol-specific phospholipase C (PI-PLC) and the isolation pattern of Listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3 x 10(9) cells each of pathogenic L. monocytogenes. Antibodies to PI-PLC appeared by 4-8 days post infection (PI)
S P, Chaudhari   +2 more
openaire   +2 more sources

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