Results 91 to 100 of about 1,118,779 (342)

Regulation of Type III Secretion System in Pseudomonas syringae.

Environmental Microbiology, 2019
Pseudomonas syringae is a model phytopathogenic bacterium that uses the type III secretion system (T3SS) to cause lethal diseases in staple crops and thus presents a threat to food security worldwide.
Yingpeng Xie, Xiaolong Shao, X. Deng
semanticscholar   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

Insights into the Bacterial Type III Secretion System

International Journal of Bio-Resource and Stress Management, 2022
Bacterial type III secretions system (T3SS) is a membrane embedded needle like macromolecular complex structure present in Gram negative bacteria and mostly found in Yersinia, Salmonella, Shigella, Entero Pathogenic E. coli (EPEC), Entero Haemorrhagic E.
Himasri Das, Chandrani Goswami, Losa Rose, Sophia M Gogoi, Durlav P Bora, Girindra K Saikia   +5 more
doaj  

High-Throughput Screening of Type III Secretion Determinants Reveals a Major Chaperone-Independent Pathway

mBio, 2018
Numerous Gram-negative bacterial pathogens utilize type III secretion systems (T3SSs) to inject tens of effector proteins directly into the cytosol of host cells.
Nadja Heinz Ernst, Analise Z. Reeves, Julia E. Ramseyer, Cammie F. Lesser   +3 more
doaj   +1 more source

Global impact of Salmonella type III secretion effector SteA on host cells [PDF]

, 2014
Salmonella enterica is a Gram-negative bacterium that causes gastroenteritis, bacteremia and typhoid fever in several animal species including humans. Its virulence is greatly dependent on two type III secretion systems, encoded in pathogenicity islands ...
Cardenal Muñoz, Elena, Gutiérrez Pozo, Gabriel, Ramos Morales, Francisco   +2 more
core   +1 more source

A NanoLuc luciferase-based assay enabling the real-time analysis of protein secretion and injection by bacterial type III secretion systems

bioRxiv, 2019
The elucidation of the molecular mechanisms of secretion through bacterial protein secretion systems is impeded by a lack of assays to quantitatively assess secretion kinetics.
Sibel Westerhausen, M. Nowak, C. Torres-Vargas, U. Bilitewski, E. Bohn, I. Grin, Samuel Wagner   +6 more
semanticscholar   +1 more source

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

An indirect enzyme-linked immunosorbent assay for rapid and quantitative assessment of Type III virulence phenotypes of Pseudomonas aeruginosa isolates

Annals of Clinical Microbiology and Antimicrobials, 2005
Background The presence of a Type III secretion system in clinical isolates of Pseudomonas aeruginosa is associated with severe disease and poor outcomes in infections caused by this pathogen.
Kar Kalipada, Ledizet Michel, Li Li, Koski Raymond A, Kazmierczak Barbara I   +4 more
doaj   +1 more source

RNA Type III Secretion Signals That Require Hfq [PDF]

Journal of Bacteriology, 2013
ABSTRACT Salmonella virulence is largely mediated by two type III secretion systems (T3SS) that deliver effector proteins from the bacterium to a host cell; however, the secretion signal is poorly defined. Effector N termini are thought to contain the signal, but they lack homology, possess no identifiable motif, and ...
George S, Niemann, Roslyn N, Brown, Ivy T, Mushamiri, Nhu T, Nguyen, Rukayat, Taiwo, Afke, Stufkens, Richard D, Smith, Joshua N, Adkins, Jason E, McDermott, Fred, Heffron   +9 more
openaire   +2 more sources

The type III secretion system needle, tip, and translocon

Protein Science, 2019
Many Gram‐negative bacteria pathogenic to plants and animals deploy the type III secretion system (T3SS) to inject virulence factors into their hosts.
Supratim Dey, Amritangshu Chakravarty, Pallavi Guha Biswas, Roberto N De Guzman   +3 more
semanticscholar   +1 more source