Results 81 to 90 of about 362,571 (177)

An intracellular recombinant single‐chain variable antibody fragment as a new class of phosphodiesterase type 5 inhibitors

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a ubiquitous second messenger involved in human (patho‐)physiology. Phosphodiesterase 5 (PDE5) is a major cGMP hydrolyzing enzyme in many cell types including vascular smooth muscle cells (VSMCs). Several highly selective PDE5 inhibitors are in clinical use. However, there are currently no
Kürsat Kirkgöz, Sophie Sprenger, Olga Schweigert, Sanika Mohagaonkar, Matti Walsdorff, Tanja Zeller, Filip Berisha, Viacheslav O. Nikolaev, Sergei D. Rybalkin   +8 more
wiley   +1 more source

Evidence That the Protein Phosphatase Activity of PTEN Contributes to Embryonic Development and Tumor Suppression

Cancer Science, EarlyView.
Mice expressing only mutant PTEN Y138L, a protein which shows normal suppression of cellular AKT yet lacks protein phosphatase activity, die in utero, and heterozygous mice display a range of tumors. This indicates both the lipid and protein phosphatase activities of PTEN work together for normal tumor suppression and embryonic development.
Priyanka Tibarewal, Laura Spinelli, Nisha Kriplani, Helen Wise, Nadege Poncet, Giulia Marzano, Karen E. Anderson, Katarzyna M. Grzes, Zofia Varyova, Mahreen Adil, C. Peter Downes, Phillip T. Hawkins, Len R. Stephens, Kate G. Storey, Doreen A. Cantrell, Bart Vanhaesebroeck, Nicholas R. Leslie   +16 more
wiley   +1 more source

The genome of the brown alga Ectocarpus siliculosus contains a series of viral DNA pieces, suggesting an ancient association with large dsDNA viruses

BMC Evolutionary Biology, 2008
Background Ectocarpus siliculosus virus-1 (EsV-1) is a lysogenic dsDNA virus belonging to the super family of nucleocytoplasmic large DNA viruses (NCLDV) that infect Ectocarpus siliculosus, a marine filamentous brown alga. Previous studies indicated that
Boland Wilhelm, Delaroque Nicolas
doaj   +1 more source

From biology to biotechnology: Host‐regulation factors from parasitoid wasps are a source of bioactive molecules with translational potential

Insect Molecular Biology, EarlyView.
Parasitoid wasps deploy maternal and embryonic factors to reprogramme host physiology. Venom, calyx fluid, polydnaviruses, teratocytes and larval secretions act in a coordinated, compartmentalised manner. Host‐regulation factors are promising sources of insecticidal, antimicrobial and bioinspired translational molecules.
Ciro Pedro G. Pinto, Andrew A. Walker, Glenn F. King   +2 more
wiley   +1 more source

Intrinsic pH chemosensitivity of acutely dissociated medullary serotonergic and retrotrapezoid neurones

The Journal of Physiology, EarlyView.
Abstract figure legend Central respiratory chemoreceptors (CRCs) are critical for maintaining normal systemic levels of pH and PCO2. A CRC must respond to pH via intrinsic mechanisms. Here we quantified intrinsic chemosensitivity of medullary 5‐HT neurones and retrotrapezoid nucleus (RTN) neurones using acute dissociation.
Yuanming Wu, Eduardo Bravo, George Bradley Richerson   +2 more
wiley   +1 more source

A TREK‐1/AQP4/TRPA1/BDNF Signaling Axis Is Associated With Astrocytic Volume Transients, Synaptic Plasticity, and Spatial Memory

Glia, Volume 74, Issue 9, September 2026.
Astrocytic TREK‐1‐dependent volume transients contribute to hippocampal synaptic plasticity and memory formation. TRPA1‐mediated astrocytic calcium signaling is required for long‐term potentiation, while astrocytic BDNF is necessary and sufficient to support sustained synaptic plasticity.
Victor James Drew, Junsung Woo, Jung Moo Lee, Wuhyun Koh, Joungha Won, Jae‐Hun Lee, C. Justin Lee   +6 more
wiley   +1 more source

Advances in large DNA fragment assembly for microbial cell factory engineering

Quantitative Biology, Volume 14, Issue 3, September 2026.
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang, Wenqian Liu, Dongyuan Cheng, Jiazhang Lian, Xian Fu, Yue Shen   +5 more
wiley   +1 more source

Site-Specific Recombination at XerC/D Sites Mediates the Formation and Resolution of Plasmid Co-integrates Carrying a blaOXA-58- and TnaphA6-Resistance Module in Acinetobacter baumannii

Frontiers in Microbiology, 2018
Members of the genus Acinetobacter possess distinct plasmid types which provide effective platforms for the acquisition, evolution, and dissemination of antimicrobial resistance structures.
María M. Cameranesi, Jorgelina Morán-Barrio, Adriana S. Limansky, Guillermo D. Repizo, Alejandro M. Viale   +4 more
doaj   +1 more source

Writing Big in Plant Genomes: Advances, Challenges and Strategies for Targeted Large‐Fragment DNA Insertion

Plant, Cell &Environment, Volume 49, Issue 9, Page 6506-6520, September 2026.
ABSTRACT Precise genome editing has transformed plant biology and crop improvement by enabling targeted modification of endogenous loci. Beyond gene knockout and base editing, the site‐specific insertion of exogenous DNA, particularly large DNA fragments, has become a central goal for engineering complex traits, reconstructing metabolic pathways and ...
Fengfeng Zhang, Daqi Yan, Jiayi Hou, Dan Yang, Yan Xiong, Mingzhang Wen, Xiaoyue Zhu   +6 more
wiley   +1 more source

Role of phase-variable Mfa1 fimbriae of Phocaeicola vulgatus in its adhesion and proinflammatory activity toward gut epithelial cells

Gut Microbes Reports
Bacteroidaceae is a prominent family in the human gut microbiota. The bacteria within this family are characterized by phase variable regulation of diverse surface structures, such as capsular polysaccharides and outer membrane proteins.
Emmanuel Munyeshyaka, Haruyuki Nakayama-Imaohji, Hisashi Yamasaki, Ayano Tada, Nafisa Tabassum, Tomomi Kuwahara   +5 more
doaj   +1 more source