Results 71 to 80 of about 179,081 (177)

Low‐Concentration Enhancement Effect: Enabling Sensitive Detection of EML4‐ALK Variants via Multiplex RT‐MIRA

Journal of Clinical Laboratory Analysis, EarlyView.
This study developed an optimized multiplex RT‐MIRA assay for the simultaneous detection of EML4‐ALK variants, achieving single‐copy sensitivity. Unexpectedly, during the development of the reaction system, we discovered a “low‐concentration enhancement effect,” where reducing primer or probe concentrations within a specific range markedly improved ...
Xiao‐Dong Ren, Xi‐Yao Luo, Jin‐Mi Li, Xian‐Ge Sun, Jing Lv, Hua‐Liang Xiao, Qiu‐Shi Wang, Hong Wang, Chun‐Yan Wang, Hao‐Zheng Li, Qing Huang   +10 more
wiley   +1 more source

Requirements for Site-Specific Recombination in the Tyrosine-Family Recombinase Active Site [PDF]

Biophysical Journal, 2014
Tyrosine recombinase (YR) shares the same active site and catalyzes the DNA rearrangement with identical phosphoryl transfer chemistry to topoisomerases IB (TopIBs). In order to find out the function of each residue within conserved catalytic center and what factor dictates the strikingly different kinetic characteristics, in cis for Cre and λ Int and ...
openaire   +1 more source

From knowledge to action: the importance of characterizing herbicide resistance mechanisms for implementing management strategies

Pest Management Science, EarlyView.
This review bridges the gap between understanding resistance mechanisms and applying them in weed management, highlighting how mechanistic insights reveal vulnerabilities that can guide more effective control strategies. Abstract Herbicide resistance remains one of the most intensively studied areas in weed science because of its direct consequences ...
Luan Cutti, Eric L. Patterson, Todd A. Gaines, Aldo Merotto   +3 more
wiley   +1 more source

Protein tyrosine phosphatase TbPTP1:A molecular switch controlling life cycle differentiation in trypanosomes [PDF]

, 2006
Differentiation in African trypanosomes (Trypanosoma brucei) entails passage between a mammalian host, where parasites exist as a proliferative slender form or a G0-arrested stumpy form, and the tsetse fly.
Szoor, Balazs; id_orcid, Matthews, Keith R., Helen McElhinney, Tabernero, Lydia, Wilson, Jude, Szöor, Balázs, Keith R. Matthews, Matthews, Keith R; id_orcid, Lydia Tabernero, Tabernero, Lydia; id_orcid, McElhinney, Helen, Balázs Szöőr, Jude Wilson   +12 more
core   +1 more source

Functional analysis of box I mutations in yeast site-specific recombinases Flp and R: pairwise complementation with recombinase variants lacking the active-site tyrosine. [PDF]

Molecular and Cellular Biology, 1992
The site-specific recombinases Flp and R from Saccharomyces cerevisiae and Zygosaccharomyces rouxii, respectively, are related proteins that belong to the yeast family of site-specific recombinases. They share approximately 30% amino acid matches and exhibit a common reaction mechanism that appears to be conserved within the larger integrase family of ...
J W, Chen, B R, Evans, S H, Yang, H, Araki, Y, Oshima, M, Jayaram   +5 more
openaire   +2 more sources

Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi

Yeast, EarlyView.
Applications of transposon‐based tools for genome engineering in fungi include transposon‐mediated mutagenesis and targeted genomic integration. ABSTRACT Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes.
Bingyin Peng, Masahiro Tominaga, Chengqiang Wang, Robert E. Speight, Jun Ishii   +4 more
wiley   +1 more source

An intracellular recombinant single‐chain variable antibody fragment as a new class of phosphodiesterase type 5 inhibitors

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a ubiquitous second messenger involved in human (patho‐)physiology. Phosphodiesterase 5 (PDE5) is a major cGMP hydrolyzing enzyme in many cell types including vascular smooth muscle cells (VSMCs). Several highly selective PDE5 inhibitors are in clinical use. However, there are currently no
Kürsat Kirkgöz, Sophie Sprenger, Olga Schweigert, Sanika Mohagaonkar, Matti Walsdorff, Tanja Zeller, Filip Berisha, Viacheslav O. Nikolaev, Sergei D. Rybalkin   +8 more
wiley   +1 more source

Long non‐coding RNAs at the crossroads of inflammation, cancer and angiogenesis: Molecular mechanisms and their potential as therapeutic targets

British Journal of Pharmacology, EarlyView.
Long non‐coding RNAs (lncRNAs), a broad class of non‐protein‐coding RNAs, are characterized as new regulators of gene expression at the epigenetic, transcriptional, and post‐transcriptional level. Thus, lncRNAs are involved in the regulation of physiological processes and the development of human diseases and cancer by modulating proinflammatory ...
Charlie Leboff, Pamela Calle‐Mendoza, Maria Hatziapostolou, Christos Polytarchou   +3 more
wiley   +1 more source

Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways

The site-specific recombinase Cre was previously reported to have in vitro activity. Here, we describe the method of purifying two new tyrosine site-specific recombinases VCre and Dre along with Cre by nickel affinity chromatography.
Tuck L, Marles-Wright J, Cai Y, Liu W
core   +4 more sources

Evidence That the Protein Phosphatase Activity of PTEN Contributes to Embryonic Development and Tumor Suppression

Cancer Science, EarlyView.
Mice expressing only mutant PTEN Y138L, a protein which shows normal suppression of cellular AKT yet lacks protein phosphatase activity, die in utero, and heterozygous mice display a range of tumors. This indicates both the lipid and protein phosphatase activities of PTEN work together for normal tumor suppression and embryonic development.
Priyanka Tibarewal, Laura Spinelli, Nisha Kriplani, Helen Wise, Nadege Poncet, Giulia Marzano, Karen E. Anderson, Katarzyna M. Grzes, Zofia Varyova, Mahreen Adil, C. Peter Downes, Phillip T. Hawkins, Len R. Stephens, Kate G. Storey, Doreen A. Cantrell, Bart Vanhaesebroeck, Nicholas R. Leslie   +16 more
wiley   +1 more source