Results 71 to 80 of about 362,571 (177)

Comprehensive prediction of chromosome dimer resolution sites in bacterial genomes

BMC Genomics, 2011
Background During the replication process of bacteria with circular chromosomes, an odd number of homologous recombination events results in concatenated dimer chromosomes that cannot be partitioned into daughter cells.
Arakawa Kazuharu, Kono Nobuaki, Tomita Masaru   +2 more
doaj   +1 more source

Mutational Analysis of the Archaeal Tyrosine Recombinase SSV1 Integrase Suggests a Mechanism of DNA Cleavage in trans [PDF]

Journal of Biological Chemistry, 2004
The only tyrosine recombinase so far studied in archaea, the SSV1 integrase, harbors several changes in the canonical residues forming the catalytic pocket of this family of recombinases. This raised the possibility of a different mechanism for archaeal tyrosine recombinase.
Claire, Letzelter, Michel, Duguet, Marie-Claude, Serre   +2 more
openaire   +2 more sources

The Natural Product Corramycin Acts as a DNA Gyrase Poison and Overcomes Fluoroquinolone Resistance in Mycobacterium tuberculosis

Advanced Science, EarlyView.
Corramycin, a myxobacterial natural product antibiotic, exhibits potent bactericidal activity against multidrug‐resistant Mycobacterium tuberculosis. The compound hijacks bacterial transporters such as BacA and OppABCD to enter the cell and inhibits DNA synthesis through a previously unrecognized mode of DNA gyrase poisoning, locking the enzyme in an ...
Franziska Fries, Jennifer Herrmann, Michael Dal Molin, Yaëlle Wormser, Timo Risch, Yunsheng Gao, Gareth Prosser, Francesca Gubellini, Ariel Mechaly, Joshua B. Wallach, Anthony Castro, Arka Banerjee, Sari Rasheed, Alexander Horn, Mathias Müsken, Michael Kurz, Lindsay Sonnenkalb, Viola Dreyer, Stefan Niemann, Sönke Andres, Dirk Schnappinger, Uli Kazmaier, Norbert Reiling, Rolf Müller, Jan Rybniker, Stéphanie Petrella   +25 more
wiley   +1 more source

Four Chromosomal Type IV Secretion Systems in Helicobacter pylori: Composition, Structure and Function

Frontiers in Microbiology, 2020
The pathogenic bacterium Helicobacter pylori is genetically highly diverse and a major risk factor for the development of peptic ulcer disease and gastric adenocarcinoma in humans. During evolution, H.
Wolfgang Fischer, Nicole Tegtmeyer, Kerstin Stingl, Steffen Backert   +3 more
doaj   +1 more source

Loss of SHP1 in Spinal Astrocytes Triggers T‐Lymphocyte Infiltration and Nociceptive Hypersensitivity

Advanced Science, EarlyView.
Astrocyte‐specific SHP1 deletion disrupts astrocyte–vascular interactions and compromises BBB integrity while enhancing STAT1‐dependent CXCL10 expression. These changes synergistically promote peripheral CD4+ and CD8+ T‐cell infiltration into the SDH. The infiltrating T cells secrete IFN‐γ, which in turn activates microglia.
Lan‐Xing Yi, Lin Yang, Kang‐Li Wang, Hui‐Zhu Liu, Rui‐Ying Chen, Min Su, Xiao Xiao, Yu‐Qiu Zhang   +7 more
wiley   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Requirements for Site-Specific Recombination in the Tyrosine-Family Recombinase Active Site [PDF]

Biophysical Journal, 2014
Tyrosine recombinase (YR) shares the same active site and catalyzes the DNA rearrangement with identical phosphoryl transfer chemistry to topoisomerases IB (TopIBs). In order to find out the function of each residue within conserved catalytic center and what factor dictates the strikingly different kinetic characteristics, in cis for Cre and λ Int and ...
openaire   +1 more source

Expression of mutant TIE2 p.L914F during mouse development causes embryonic lethality and defects in vascular remodeling

Developmental Dynamics, EarlyView.
Abstract Background Sporadic venous malformation (VM) is associated with the hyperactivating p.L914F mutation in TIE2, a receptor tyrosine kinase essential for vascular development. This mutation is not found in hereditary VM, suggesting incompatibility with life when expressed during early vascular development.
Lindsay J. Bischoff, Sandra Schrenk, Kara Soroko, Chhiring Sherpa, Ashok Arasu, Damien Reynaud, Elisa Boscolo   +6 more
wiley   +1 more source

Biochemical and Kinetic Analysis of the RNase Active Sites of the Integrase/Tyrosine Family Site-specific DNA Recombinases*

Journal of Biological Chemistry, 2001
In this study, we have used multiple strategies to characterize the mechanisms of the type I and type II RNA cleavage activities harbored by the Flp (pronounced here as “flip”) site-specific DNA recombinase (Flp-RNase I and II, respectively).
A. Sau, G. Tribble, I. Grainge, R. Frøhlich, B. Knudsen, M. Jayaram   +5 more
semanticscholar   +1 more source

Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi

Yeast, EarlyView.
Applications of transposon‐based tools for genome engineering in fungi include transposon‐mediated mutagenesis and targeted genomic integration. ABSTRACT Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes.
Bingyin Peng, Masahiro Tominaga, Chengqiang Wang, Robert E. Speight, Jun Ishii   +4 more
wiley   +1 more source