Results 81 to 90 of about 27,627 (205)

Three-dimensional structure of a ubiquitin-conjugating enzyme (E2).

Journal of Biological Chemistry, 1992
The x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme (E2) encoded by the UBC1 gene of the plant Arabidopsis thaliana has been determined with the use of multiple isomorphous replacement techniques and refined at 2.4-A resolution by simulated annealing and restrained least-squares.
R.D. Vierstra, M.L. Sullivan, Leigh C. Jeffrey, William J. Cook   +3 more
openaire   +3 more sources

Single-substrate Enzyme Kinetics: The Quasi-steady-state Approximation and Beyond [PDF]

J. Math. Chem. 51 (2013) 1467, 2012
We analyze the standard model of enzyme-catalyzed reactions at various substrate-enzyme ratios to identify the regions of validity of the quasi-steady-state approximation. Certain prevalent conditions are checked and compared against the actual findings. Efficacies of a few other measures are highlighted. Some very recent observations are rationalized,
arxiv  

Effective inter-symbol interference mitigation with a limited amount of enzymes in molecular communications [PDF]

arXiv, 2016
In molecular communication via diffusion (MCvD), the inter-symbol interference (ISI) is a well known severe problem that deteriorates both data rates and link reliability. ISI mainly occurs due to the slow and highly random propagation of the messenger molecules, which causes the emitted molecules from the previous symbols to interfere with molecules ...
arxiv  

Structures, Functions, and Mechanisms of Filament Forming Enzymes: A Renaissance of Enzyme Filamentation [PDF]

arXiv, 2019
Filament formation by non-cytoskeletal enzymes has been known for decades, yet only relatively recently has its wide-spread role in enzyme regulation and biology come to be appreciated. This comprehensive review summarizes what is known for each enzyme confirmed to form filamentous structures in vitro, and for the many that are known only to form large
arxiv  

Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination

Nature Communications, 2020
The Legionella pneumophila effector MavC inhibits the human ubiquitin-conjugating enzyme Ube2N. Here, the authors combine NMR, X-ray crystallography and biochemical assays and show that MavC catalyses the intramolecular transglutaminase reaction between ...
Kedar Puvar, Shalini Iyer, Jiaqi Fu, Sebastian Kenny, Kristos I. Negrón Terón, Zhao-Qing Luo, Peter S. Brzovic, Rachel E. Klevit, Chittaranjan Das   +8 more
doaj   +1 more source

Identification of functionally related enzymes by learning-to-rank methods [PDF]

arXiv, 2014
Enzyme sequences and structures are routinely used in the biological sciences as queries to search for functionally related enzymes in online databases. To this end, one usually departs from some notion of similarity, comparing two enzymes by looking for correspondences in their sequences, structures or surfaces. For a given query, the search operation
arxiv  

The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis via blocking inactive rhomboid protein 2-dependent pathway

Acta Pharmaceutica Sinica B, 2023
Nowadays potential preclinical drugs for the treatment of nonalcoholic steatohepatitis (NASH) have failed to achieve expected therapeutic efficacy because the pathogenic mechanisms are underestimated.
Minxuan Xu, Jun Tan, Liancai Zhu, Chenxu Ge, Wei Dong, Xianling Dai, Qin Kuang, Shaoyu Zhong, Lili Lai, Chao Yi, Qiang Li, Deshuai Lou, Linfeng Hu, Xi Liu, Gang Kuang, Jing Luo, Jing Feng, Bochu Wang   +17 more
doaj  

Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting

STAR Protocols, 2022
Summary: Ubiquitin-fold modifier 1 (UFM1) system is a recently identified ubiquitin-like modification with essential biological functions. Similar to ubiquitination, the covalent conjugation of UFM1 (UFMylation) to target proteins involves a three-step ...
Junzhi Zhou, Qian Liang, Maogong Dong, Xiaohe Ma, Yaqi Jin, Di Guan, Jiang Liu, Miao Wang, Yu-sheng Cong   +8 more
doaj  

Role of RING-Type E3 Ubiquitin Ligases in Inflammatory Signalling and Inflammatory Bowel Disease

Mediators of Inflammation, 2020
Ubiquitination is a three-step enzymatic cascade for posttranslational protein modification. It includes the ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2), and ubiquitin ligase (E3).
Liguo Zhu, Ying Li, Longyuan Zhou, Guang Yang, Ying Wang, Jing Han, Li Li, Shenghong Zhang   +7 more
doaj   +1 more source

Combined Genetic Association and Differed Expression Analysis of UBE2L3 Uncovers a Genetic Regulatory Role of (Immuno)proteasome in IgA Nephropathy

Kidney Diseases
Introduction: IgA nephropathy (IgAN) is a leading cause of end-stage renal disease. The exact pathogenesis of IgAN is not well defined, but some genetic studies have led to a novel discovery that the (immuno)proteasome probably plays an important role in
Lin-Lin Xu, Ting Gan, Yang Li, Pei Chen, Su-Fang Shi, Li-Jun Liu, Ji-Cheng Lv, Hong Zhang, Xu-Jie Zhou   +8 more
doaj   +1 more source