Dynamic control of selectivity in the ubiquitination pathway revealed by an ASP to GLU substitution in an intra-molecular salt-bridge network [PDF]
, 2012 Ubiquitination relies on a subtle balance between selectivity and promiscuity achieved through specific interactions between ubiquitin-conjugating enzymes (E2s) and ubiquitin ligases (E3s).
Bonvin, Alexandre M. J. J. +4 more
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Frontiers in Genetics, 2021 Notch signaling pathway plays crucial roles in animal development. Protein ubiquitination contributes to Notch signaling regulation by governing the stability and activity of major signaling components.
Fengchao Zhang +3 more
doaj +1 more source
Phenotypic Characterization of the Arabidopsis ufm1 (Ubiquitin Fold Modifier) Gene Involved in Seed Development [PDF]
, 2016 Background and Objective: With the completion of the Arabidopsis genome sequencing, the next challenge is the determination ofgene function. Post-translational modifications of proteins by small polypeptide are implicated in plant growth and development ...
Cornejo, Paula +1 more
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Interplay between the Ubiquitin Proteasome System and Ubiquitin-Mediated Autophagy in Plants
Cells, 2020 All eukaryotes rely on the ubiquitin-proteasome system (UPS) and autophagy to control the abundance of key regulatory proteins and maintain a healthy intracellular environment. In the UPS, damaged or superfluous proteins are ubiquitinated and degraded in
Tong Su +4 more
doaj +1 more source
Poxvirus Interactions with the Host Ubiquitin System
Pathogens, 2021 The ubiquitin system has emerged as a master regulator of many, if not all, cellular functions. With its large repertoire of conjugating and ligating enzymes, the ubiquitin system holds a unique mechanism to provide selectivity and specificity in ...
Sian Lant, Carlos Maluquer de Motes
doaj +1 more source
E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. [PDF]
Nat Struct Mol Biol, 2013 OTUB1 is a Lys48-specific deubiquitinating enzyme that forms a complex in vivo with E2 ubiquitin (Ub)-conjugating enzymes including UBC13 and UBCH5. OTUB1 binds E2~Ub thioester intermediates and prevents ubiquitin transfer, thereby noncatalytically inhibiting accumulation of polyubiquitin.
Wiener R +6 more
europepmc +4 more sources
Biochemical and Genetic Studies of UBR3, a Ubiquitin Ligase with a Function in Olfactory and Other Sensory Systems [PDF]
, 2007 Our previous work identified E3 ubiquitin ligases, termed UBR1-UBR7, that contain the ~70-residue UBR box, a motif important for the targeting of N-end rule substrates.
Hellweg, Rainer +6 more
core +1 more source
A Yeast-Based Functional Assay to Study Plant N-Degron – N-Recognin Interactions
Frontiers in Plant Science, 2022 The N-degron pathway is a branch of the ubiquitin-proteasome system where amino-terminal residues serve as degradation signals. In a synthetic biology approach, we expressed ubiquitin ligase PRT6 and ubiquitin conjugating enzyme 2 (AtUBC2) from ...
Aida Kozlic +11 more
doaj +1 more source
Nature Communications, 2017 The transfer of ubiquitin (UB) to cellular targets is mediated sequentially by three groups of enzymes, UB activating enzyme (E1), UB conjugating enzyme (E2) and UB ligase (E3).
Xianpeng Liu +10 more
doaj +1 more source
Mechanisms of Generating Polyubiquitin Chains of Different Topology
Cells, 2014 Ubiquitination is an important post-translational process involving attachment of the ubiquitin molecule to lysine residue/s on a substrate protein or on another ubiquitin molecule, leading to the formation of protein mono-, multi- or polyubiquitination.
Randy Suryadinata +3 more
doaj +1 more source