Results 71 to 80 of about 280,422 (406)
Ubiquitination and SUMOylation, which are posttranslational modifications, play prominent roles in regulating both protein expression and function in cells, as well as various cellular signal transduction pathways. Metabolic reprogramming often occurs in
Shunqin Zhu+9 more
doaj +1 more source
Translesion synthesis in mammalian cells [PDF]
DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialized low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five
Alan R. Lehmann+35 more
core +2 more sources
Evolution of the Ubiquitin-Activating Enzyme Uba1 (E1) [PDF]
Ubiquitin tags diseased proteins and initiates an enzyme conjugation cascade, which has three stages. The first-stage enzyme Uba1 (E1) has evolved only modestly from slime mold to humans, and is > 14 times larger than Ub. Here we use critical point thermodynamic scaling theory to connect Uba1 (E1) evolution from yeast and slime mold to fruit flies and ...
arxiv +1 more source
Force-clamp analysis techniques reveal stretched exponential unfolding kinetics in ubiquitin [PDF]
Force-clamp spectroscopy reveals the unfolding and disulfide bond rupture times of single protein molecules as a function of the stretching force, point mutations and solvent conditions. The statistics of these times reveal whether the protein domains are independent of one another, the mechanical hierarchy in the polyprotein chain, and the functional ...
arxiv +1 more source
Dengue Virus Genome Uncoating Requires Ubiquitination [PDF]
The process of genome release or uncoating after viral entry is one of the least-studied steps in the flavivirus life cycle. Flaviviruses are mainly arthropod-borne viruses, including emerging and reemerging pathogens such as dengue, Zika, and West Nile ...
Byk, Laura Andrea+5 more
core +3 more sources
Using Ubiquitin Binders to Decipher the Ubiquitin Code [PDF]
Post-translational modifications (PTMs) by ubiquitin (Ub) are versatile, highly dynamic, and involved in nearly all aspects of eukaryote biological function. The reversibility and heterogeneity of Ub chains attached to protein substrates have complicated their isolation, quantification, and characterization.
Mattern M+4 more
openaire +3 more sources
The MX-Helix of Muscle nAChR Subunits Regulates Receptor Assembly and Surface Trafficking. [PDF]
Nicotinic acetylcholine receptors (AChRs) are pentameric channels that mediate fast transmission at the neuromuscular junction (NMJ) and defects in receptor expression underlie neuromuscular disorders such as myasthenia gravis and congenital myasthenic ...
Borges, Lucia Soares+3 more
core
The MEKK1 PHD ubiquitinates TAB1 to activate MAPKs in response to cytokines. [PDF]
Unlike the other MAP3Ks, MEKK1 (encoded by Map3k1) contains a PHD motif. To understand the role of this motif, we have created a knockin mutant of mouse Map3k1 (Map3k1(m) (PHD)) with an inactive PHD motif.
Anwar, Saba+5 more
core +2 more sources
Regulation of the linear ubiquitination of STAT1 controls antiviral interferon signaling
Linear ubiquitination is a critical regulator of inflammatory signaling pathways. However, linearly ubiquitinated substrates and the biological significance of linear ubiquitination is incompletely understood.
Yibo Zuo+13 more
semanticscholar +1 more source
Making tau amyloid models in vitro: a crucial and underestimated challenge
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley +1 more source