Results 71 to 80 of about 44,652 (214)

Rational design of a highly active N-glycosyltransferase mutant using fragment replacement approach

Engineering Microbiology
The modularity of carbohydrate-active enzymes facilitates that enzymes with different functions have similar fragments. However, because of the complex structure of the enzyme active sites and the epistatic effects of various mutations on enzyme activity,
Jiangyu Yang, Kun Li, Yongheng Rong, Zhaoxi Liu, Xiaoyu Liu, Yue Yu, Wenjing Shi, Yun Kong, Min Chen   +8 more
doaj   +1 more source

Functional and transcriptomic analyses in Neurospora crassa reveal a crucial role for the N‐glycoprotein deglycosylation process in fungal homeostasis

The FEBS Journal, EarlyView.
In Neurospora crassa, a cytosolic GH18 endo‐β‐n‐acetylglucosaminidase (ENGase) functions as an active deglycosylating enzyme. Deletion of this severely alters tolerance to multiple stress conditions and causes extensive transcriptomic reprogramming, including upregulation of a GH20 exo‐β‐n‐acetylhexosaminidase under ER stress, indicating that N. crassa
Anastasios Samaras, Tanim Jabid Hossain, Magnus Karlsson, Georgios Tzelepis   +3 more
wiley   +1 more source

O-Glycosylation of thrombospondin type 1 repeats : identification and characterisation of β1,3-glucosyltransferase [PDF]

, 2008
Rare types of protein glycosylation often occur in a domain-specific manner required for important biological functions (Okajima et al. 2008). O-linked fucosylation, where the fucose is linked directly to the hydroxyl groups of serine or threonine ...
Keusch, Krisztina
core   +1 more source

Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered E. coli

Bioresources and Bioprocessing
Defining suitable enzymes for reaction steps in novel synthetic pathways is crucial for developing microbial cell factories for non-natural products. Here, we developed a computational workflow to identify C12 alcohol-active UDP-glycosyltransferases. The
Kasimaporn Promubon, Kritsada Tathiya, Aussara Panya, Wasu Pathom-Aree, Pachara Sattayawat   +4 more
doaj   +1 more source

Genome assembly of Elcysma westwoodi provides insights into host‐plant cyanogenic glucoside detoxification and chemical defence gene evolution

Insect Molecular Biology, EarlyView.
A chromosome‐level genome of Elcysma westwoodi was assembled. The genome encodes 272 detoxification‐related genes. A single‐copy bCAS was retained for cyanide detoxification. CYP405A and CYP332A were recruited into cyanogenic defence evolution. UGT33A retained a conserved GT‐B fold and catalytic domains.
Minyoung Choi, Murtaza Khan, Juil Kim
wiley   +1 more source

Phylogenetic Analysis of the UDP-glycosyltransferase Multigene Family of Arabidopsis thaliana [PDF]

Journal of Biological Chemistry, 2001
A class of UDP-glycosyltransferases (UGTs) defined by the presence of a C-terminal consensus sequence is found throughout the plant and animal kingdoms. Whereas mammalian enzymes use UDP-glucuronic acid, the plant enzymes typically use UDP-glucose in the transfer reactions. A diverse array of aglycones can be glucosylated by these UGTs.
Y, Li, S, Baldauf, E K, Lim, D J, Bowles
openaire   +2 more sources

CP32, a novel anti‐oomycete cyclic peptide, interferes with cell‐wall deposition in Phytophthora infestans

Journal of Integrative Plant Biology, EarlyView.
The small cyclic peptide CP32 effectively inhibits Phytophthora infestans, the causal agent of potato and tomato late blight. Combined biochemical and phenotypic analyses demonstrate that CP32 disrupts cell wall biosynthesis, a key process in pathogen infection, resulting in a marked reduction of disease symptoms in plants.
Demetrio Marcianò, Stefano Rosa, Elena Marone Fassolo, Chiara Bertaso, Andrea Tagliani, Nina Gauri Capra, Federico Ballabio, Chiara Mizzotti, Giulia Rizzi, Sadia Fida Ullah, Lisa Kappel, Lucia Feni, Luca Tadini, Louise Jane Gourlay, Rosaria Russo, Vincent Bulone, Sabrina Pricl, Sandrine Ongeri, Sara Pellegrino, Carlo Camilloni, Silvia Laura Toffolatti, Vaibhav Srivastava, Paolo Pesaresi, Simona Masiero   +23 more
wiley   +1 more source

Genome‐scale insights into metabolic streamlining and photosynthetic energy balance in the extremophile green alga Picocystis salinarum (Picocystophyceae, Chlorophyta)

Journal of Phycology, EarlyView.
Abstract Picocystis salinarum is an early‐diverging chlorophyte and the sole described member of the Picocystophyceae, frequently dominating hypersaline and alkaline lakes despite extreme physicochemical constraints. To elucidate the genomic foundations of its ecological success, we generated a fully annotated, chromosome‐scale nuclear genome assembly ...
Claude Lemieux, Christian Otis, Monique Turmel   +2 more
wiley   +1 more source

A Mobile Glycosylation Locus Modulates Cell Wall Architecture in Lactobacillus crispatus

Molecular Microbiology, EarlyView.
Serial propagation of Lactobacillus crispatus triggers transposon‐mediated loss of a mobile glycosylation gene fragment (GT3), destabilising the glycosyltransferase complex and remodelling the cell wall with 20%–25% thinner peptidoglycan, while sugar composition remains unchanged.
P. Achterberg, M. Lekkerkerker, L. Koelemeijer, S. Illidge, J. Vroom, R. Y. Hertzberger, J. Vreede, M. C. M. van Loosdrecht, R. Kort   +8 more
wiley   +1 more source

Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae

Scientific Reports, 2017
The emergence of antibiotic-resistant pathogenic bacteria is a healthcare problem worldwide. We evaluated the antimicrobial activity of rhodomyrtone, an acylphloroglucinol present in Rhodomyrtus tomentosa leaves, against the human Gram-positive pathogen ...
Watcharapong Mitsuwan, Alfonso Olaya-Abril, Mónica Calderón-Santiago, Irene Jiménez-Munguía, José Antonio González-Reyes, Feliciano Priego-Capote, Supayang P. Voravuthikunchai, Manuel J. Rodríguez-Ortega   +7 more
doaj   +1 more source