Carbohydrate utilization in Streptococcus thermophilus: characterization of the genes for aldose 1-epimerase (mutarotase) and UDPglucose 4-epimerase. [PDF]
J Bacteriol, 1990 The complete nucleotide sequences of the genes encoding aldose 1-epimerase (mutarotase) (galM) and UDPglucose 4-epimerase (galE) and flanking regions of Streptococcus thermophilus have been determined. Both genes are located immediately upstream of the S. thermophilus lac operon.
Poolman B +3 more
europepmc +13 more sources
UDPGlucose 4-epimerase from Saccharomyces fragilis. Allosteric kinetics with UDP-glucose as substrate [PDF]
Journal of Biological Chemistry, 1975 UDPglucose 4-epimerase from Saccharomyces fragilis catalyzes a freely reversible reaction between UDP-galactose and UDP-glucose. With UDP-galactose as the substrate the enzyme shows a classical hyperbolic kinetics but when UDP-glucose is used as the substrate a distinct allostericity is observed.
M, Hay, A, Bhaduri
semanticscholar +5 more sources
Distinct functional roles of two active site thiols in UDPglucose 4-epimerase from Kluyveromyces fragilis. [PDF]
Journal of Biological Chemistry, 1992 UDPglucose 4-epimerase from Kluyveromyces fragilis was earlier shown to have two conformationally vicinal thiols at the active site. Upon treatment with diamide, these thiols form a disulfide linkage across the subunits that results in coordinated loss of catalytic activity and coenzyme fluorescence (Ray, M., and Bhaduri, A. (1980) J. Biol. Chem.
H, Bhattacharjee, A, Bhaduri
semanticscholar +5 more sources
Molecular characterisation of Entamoeba histolytica UDP-glucose 4-epimerase, an enzyme able to provide building blocks for cyst wall formation. [PDF]
PLoS Negl Trop Dis, 2023 In the human host, the protozoan parasite Entamoeba histolytica is adapted to a non-invasive lifestyle in the colon as well as to an invasive lifestyle in the mesenterial blood vessels and the liver.
Nagode A +5 more
europepmc +2 more sources
Journal of Biological Chemistry, 1992 UDPglucose 4-epimerase from Kluyveromyces fragilis was completely inactivated by diethylpyrocarbonate following pseudo-first order reaction kinetics. The pH profile of diethylpyrocarbonate inhibition and reversal of inhibition by hydroxylamine suggested specific modification of histidyl residues. Statistical analysis of the residual enzyme activity and
S, Mukherji, A, Bhaduri
openaire +3 more sources
Marine bacteria Alteromonas spp. require UDP-glucose-4-epimerase for aggregation and production of sticky exopolymer. [PDF]
mBioThe physiology and ecology of particle-associated marine bacteria are of growing interest, but our knowledge of their aggregation behavior and mechanisms controlling their association with particles remains limited.
Robertson JM +5 more
europepmc +2 more sources
Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function. [PDF]
J Biol Chem, 2020 Glycan biosynthesis relies on nucleotide sugars (NSs), abundant metabolites that serve as monosaccharide donors for glycosyltransferases. In vivo, signal-dependent fluctuations in NS levels are required to maintain normal cell physiology and are ...
Broussard A +8 more
europepmc +2 more sources
Structural basis for broad substrate specificity of UDP-glucose 4-epimerase in the human milk oligosaccharide catabolic pathway of Bifidobacterium longum. [PDF]
Sci Rep, 2019 Infant gut-associated bifidobacteria has a metabolic pathway that specifically utilizes lacto-N-biose I (Gal-β1,3-GlcNAc) and galacto-N-biose (Gal-β1,3-GalNAc) from human milk and mucin glycans. UDP-glucose 4-epimerase (GalE) from Bifidobacterium longum (
Nam YW +4 more
europepmc +2 more sources
Inherited thrombocytopenia associated with mutation of UDP-galactose-4-epimerase (GALE). [PDF]
Hum Mol Genet, 2019 Severe thrombocytopenia, characterized by dysplastic megakaryocytes and intracranial bleeding, was diagnosed in six individuals from a consanguineous kindred. Three of the individuals were successfully treated by bone marrow transplant.
Seo A +11 more
europepmc +2 more sources
The Plant Journal, 2021 Elucidating the biochemical and molecular basis of premature abscission in fruit crops should help develop strategies to enhance fruit set and yield. Here, we report that LcERF2 contributes to differential abscission rates and responses to ethylene in ...
Jun-Wen Yi +8 more
semanticscholar +1 more source