Results 251 to 260 of about 2,976,642 (307)

Ammonia Accumulation Drives Intervertebral Disc Degeneration by Triggering Ammonia‐Induced Cell Death Through Lysosome–Mitochondria Crosstalk

Cell Proliferation, EarlyView.
Mechanical strain disrupts the lysosome‐mitochondria axis via GLS1‐mediated ammonia toxicity, causing NP cell death and ECM breakdown in IVDD. CB839‐mediated GLS1 inhibition effectively rescues cellular homeostasis and alleviates disc degeneration.
Yang Zhang, Yucheng Gao, Shuhang Dong, Shuanggong Liu, Lei Liu, Ziyi Song, Jie Xu, Yijun Rong, Guangxu Song, Yingze Zhang, Dongjin Wu, Zengxin Gao   +11 more
wiley   +1 more source

From Activity Screening to Quality Control: UHPLC-MS/MS Analysis of Anti-Inflammatory Cyclodipeptides in <i>Pinellia ternata</i>. [PDF]

Molecules
Wang Y, Luo Y, Gan J, Wang L, Fang C, Zhang L, Zhen C, Chen B.   +7 more
europepmc   +1 more source

Multimodal Exploratory Analysis of Persistent Diabetic Macular Edema: Integrating Aqueous Humour Metabolomics, Clinical and OCT Features

Diabetes, Obesity and Metabolism, EarlyView.
ABSTRACT Purpose This exploratory study integrates aqueous humour metabolomic data with clinical and imaging features to examine candidate biomarkers and characterize multimodal patterns associated with persistent diabetic macular edema (pDME). Methods In this prospective cohort study, DME patients were treated with monthly anti‐VEGF injections over 3 ...
Yunhan Tao, Huimin Yu, Yunxi Ma, Mingzhu Yuan, Guochong Chen, Jun Liu, Xi Chen, Xufang Sun   +7 more
wiley   +1 more source

Deciphering the therapeutic mechanisms of Qingfei Litan prescription in acute lung injury: an integrated framework of UHPLC-MS/MS, systems pharmacology, machine learning, and <i>in vivo</i> validation. [PDF]

Front Cell Infect Microbiol
Wu W, Zhan S, Wang K.
europepmc   +1 more source

Dapagliflozin Alters Plasma Metabolome in Patients With Type 1 Diabetes During Ketosis

Diabetes, Obesity and Metabolism, EarlyView.
ABSTRACT Aims Use of sodium‐glucose cotransporter 2 inhibitors (SGLT2i) in patients with type 1 diabetes (T1D) is limited by increased risk of ketoacidosis. We aimed to determine metabolic pathways affected by SGLT2i treatment in adults with T1D during ketosis induced by insulin withdrawal.
Rosa Yang, Kevin Cho, Max C. Petersen, Kai E. Jones, Gary J. Patti, Janet B. McGill, Samantha A. DiGruccio   +6 more
wiley   +1 more source

Novel Application of UHPLC-MS, HRMS, and 2D-NMR for Structural Elucidation of Eletriptan Hydrobromide and Its Novel Degradation Products. [PDF]

Biomed Chromatogr
Bhuma DR, Maddala VKS, Salakolasu S, Katari NK.   +3 more
europepmc   +1 more source

Structural and mutational analysis of Methanosarcina mazei prenylated FMN synthase reveals the basis of its unique prenyl donor substrate specificity

The FEBS Journal, EarlyView.
Some prenylated flavin mononucleotide (prFMN) synthases exceptionally prefer dimethylallyl phosphate (DMAP) over dimethylallyl diphosphate (DMAPP), which is a common prenyl donor substrate for many prenyltransferases. Structural and mutagenic analyses of Methanosarcina mazei prFMN synthase, which prefers DMAP but can also accept DMAPP, elucidated its ...
Sou Fukuhara, Hideaki Unno, Soma Ishimine, Ryuhei Nagata, Tomokazu Ito, Hisashi Hemmi   +5 more
wiley   +1 more source

Toxicokinetic-Oriented Assessment of Nepetalactone Using In Silico ADMET Modeling, In Vitro Rat and Human Liver Microsomes, and UHPLC-MS/MS Metabolite Characterization. [PDF]

Toxics
Stoica NB, Cascajosa-Lira A, Morea A, Catunescu GM, Hornedo-Ortega R, Guzmán-Guillén R.   +5 more
europepmc   +1 more source

Dimerization of human PARP15 is required for NAD+ binding and automodification

The FEBS Journal, EarlyView.
This study provides an integrated structural model of human PARP15. Biophysical, enzymatic, and cell‐based analyses identify the dimer as the active oligomeric state capable of binding the substrate NAD+. A co‐crystal structure with a non‐hydrolyzable substrate analog BAD reveals the molecular basis for the dimerization requirement.
Anna Tuovinen, Johan Pääkkönen, Mirko M. Maksimainen, Lea Hirschen, Heli I. Hentilä, Marie Tauscher, Bernhard Lüscher, Carlos Vela‐Rodríguez, Patricia Korn, Lari Lehtiö   +9 more
wiley   +1 more source

A Method for Analysis of Free and Total Ropivacaine in Dog Plasma Using UHPLC-MS/MS. [PDF]

Biomed Chromatogr
Verdier N, Hummel K, Razzazi-Fazeli E.
europepmc   +1 more source