Results 11 to 20 of about 151,911 (185)

Accuracy of 23 Equations for Estimating LDL Cholesterol in a Clinical Laboratory Database of 5,051,467 Patients

Global Heart, 2023
Background: Alternatives to the Friedewald low-density lipoprotein cholesterol (LDL-C) equation have been proposed. Objective: To compare the accuracy of available LDL-C equations with ultracentrifugation measurement.
Christeen Samuel, Jihwan Park, Aparna Sajja, Erin D. Michos, Roger S. Blumenthal, Steven R. Jones, Seth S. Martin   +6 more
doaj   +1 more source

Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles?

Biomolecules, 2023
Mineralization-competent cells, including hypertrophic chondrocytes, mature osteoblasts, and osteogenic-differentiated smooth muscle cells secrete media extracellular vesicles (media vesicles) and extracellular vesicles bound to the extracellular matrix (
Saida Mebarek, Rene Buchet, Slawomir Pikula, Agnieszka Strzelecka-Kiliszek, Leyre Brizuela, Giada Corti, Federica Collacchi, Genevieve Anghieri, Andrea Magrini, Pietro Ciancaglini, Jose Luis Millan, Owen Davies, Massimo Bottini   +12 more
doaj   +1 more source

Proteomic and Biochemical Analysis of Extracellular Vesicles Isolated from Blood Serum of Patients with Melanoma

Separations, 2022
Background: Malignant melanoma is the most serious type of skin cancer with the highest mortality rate. Extracellular vesicles (EVs) have potential as new tumor markers that could be used as diagnostic and prognostic markers for early detection of ...
Kristiina Kurg, Anu Planken, Reet Kurg
doaj   +1 more source

Lipoprotein separation in a novel iodixanol density gradient, for composition, density, and phenotype analysis

Journal of Lipid Research, 2008
Separation of lipoproteins by traditional sequential salt density floatation is a prolonged process (∼72 h) with variable recovery, whereas iodixanol-based, self-generating density gradients provide a rapid (∼4 h) alternative.
Michael S. Yee, Darrell V. Pavitt, Tira Tan, Soundararajan Venkatesan, Ian F. Godsland, William Richmond, Desmond G. Johnston   +6 more
doaj   +1 more source

Gated Ethidium- and Bleomycin-Loading in Phage T4 That Is Subsequently Purified Leak-Free

Biophysica, 2022
Chemotherapy-inhibiting tumor cell evolution to drug-resistance is potentially suppressed by using a drug delivery vehicle (DDV) that has gating. Gating would be used to increase tumor-selectivity of delivery of DDV packaged drug.
Philip Serwer, Elena T. Wright
doaj   +1 more source

Protein concentration of synovial fluid in chronic rheumatoid arthritis. Estimation of protein in the synovial fluid of chronic rheumatoid arthritis by gel filtration and paper electrophoresis [PDF]

, 1968
For the purpose to reveal the characteris6cs of the synovial fluid of the chronic rheumatoid arthritis the proteins of the synovial fluid and blood serum have been analysed by employing the methods of electrophoresis, gel filtration on Sephadex G-200 ...
Seno, Takashi
core   +1 more source

Comparative Analysis of Natural and Cytochalasin B-Induced Membrane Vesicles from Tumor Cells and Mesenchymal Stem Cells

Current Issues in Molecular Biology, 2022
To date, there are numerous protocols for the isolation of extracellular vesicles (EVs). Depending on the isolation method, it is possible to obtain vesicles with different characteristics, enriched with specific groups of proteins, DNA and RNA, which ...
Zarema Gilazieva, Daria Chulpanova, Aleksei Ponomarev, Ivan Filin, Ekaterina Garanina, Albert Rizvanov, Valeriya Solovyeva   +6 more
doaj   +1 more source

IgA-enhancing effects of membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC15893 [PDF]

, 2018
Immunoglobulin (Ig) A in the mucus of the intestinal tract plays an important role in preventing the invasion of pathogenic microorganisms and regulating the composition of the gut microbiota.
Katakura Yoshio, Kunisawa Jun, Miyoshi Yuki, Nakayama Tomoya, Yamasaki-yashiki Shino, 中山 友哉, 山崎（屋敷） 思乃, 片倉 啓雄   +7 more
core   +2 more sources

An Integrated Approach for the Efficient Extraction and Solubilization of Rice Microsomal Membrane Proteins for High-Throughput Proteomics

Frontiers in Plant Science, 2021
The preparation of microsomal membrane proteins (MPs) is critically important to microsomal proteomics. To date most research studies have utilized an ultracentrifugation-based approach for the isolation and solubilization of plant MPs.
Truong Van Nguyen, Ravi Gupta, Dicky Annas, Jinmi Yoon, Yu-Jin Kim, Gi Hyun Lee, Jeong Woo Jang, Kang Hyun Park, Randeep Rakwal, Randeep Rakwal, Ki-Hong Jung, Cheol Woo Min, Sun Tae Kim   +12 more
doaj   +1 more source

Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK–TssFG subcomplex [PDF]

, 2014
Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However, the mechanism of the T6SS is incompletely understood. In the present study, we investigated a conserved component of
Alan R. Prescott, Aschtgen, Aschtgen, Aschtgen, Aschtgen, Ballister, Basler, Basler, Bonemann, Bonemann, Boyer, Burtnick, Casabona, Casabona, Cascales, Coulthurst, Dong, English, Felisberto-Rodrigues, Francesca R. Cianfanelli, Fritsch, Gerlach, Grant English, Holland, Hood, Jack, Kaniga, Kapitein, Kapitein, Laue, Leiman, Leiman, Lossi, Ma, Ma, Ma, MacIntyre, Mougous, Murdoch, Olwyn Byron, Pell, Potvin, Pukatzki, Rao, Rickman, Russell, Russell, Sambrook, Sarah J. Coulthurst, Schell, Schuck, Schwarz, Shneider, Silverman, Srikannathasan, Suarez, Vistica, Whitney, Zheng, Zheng, Zoued   +60 more
core   +3 more sources