Results 11 to 20 of about 151,911 (185)

The synthesis of extracellular vesicles by the protistan parasite Blastocystis

open access: yesFrontiers in Cellular and Infection Microbiology, 2022
Blastocystis is a genus of single-celled protist belonging to the stramenopile group. Prior studies have shown that isolates of Blastocystis subtype 7 (ST7) induced higher levels of intestinal epithelial cell damage and gut microbiota dysbiosis in ...
Steven Santino Leonardi   +11 more
doaj   +1 more source

Isolation and Characterization of Plasma-Derived Exosomes from the Marine Fish Rock Bream (Oplegnathus fasciatus) by Two Isolation Techniques

open access: yesFishes, 2022
Exosomes are important mediators of intercellular communication and modulate many physiological and pathological processes. Knowledge of secretion, content, and biological functions of fish exosomes during pathological infection is still scarce due to ...
Chamilani Nikapitiya   +6 more
doaj   +1 more source

Metabolomics of small extracellular vesicles derived from isocitrate dehydrogenase 1-mutant HCT116 cells collected by semi-automated size exclusion chromatography

open access: yesFrontiers in Molecular Biosciences, 2023
Cancer-derived small extracellular vesicles (sEVs) are multifunctional particles with a lipid bilayer structure that are involved in cancer progression, such as malignant proliferation, distant metastasis, and cancer immunity evasion.
Ryosuke Hayasaka   +26 more
doaj   +1 more source

Characterization of a novel type of HIV-1 particle assembly inhibitor using a quantitative Luciferase-Vpr packaging-based assay [PDF]

open access: yes, 2011
The HIV-1 auxiliary protein Vpr and Vpr-fusion proteins can be copackaged with Gag precursor (Pr55Gag) into virions or membrane-enveloped virus-like particles (VLP).
Elisabeth Errazuriz   +9 more
core   +13 more sources

New Multiscale Characterization Methodology for Effective Determination of Isolation–Structure–Function Relationship of Extracellular Vesicles

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Extracellular vesicles (EVs) have been lauded as next-generation medicines, but very few EV-based therapeutics have progressed to clinical use. Limited clinical translation is largely due to technical barriers that hamper our ability to mass produce EVs,
Thanh Huyen Phan   +19 more
doaj   +1 more source

Ready-made chromatography columns for extracellular vesicle isolation from plasma [PDF]

open access: yesJournal of Extracellular Vesicles, 2015
Proteomic studies of circulating vesicles are hampered by difficulties in purifying vesicles from plasma and serum. Isolations are contaminated with high-abundance blood proteins that may mask genuine vesicular-associated proteins and/or simply provide ...
Joanne Louise Welton   +4 more
doaj   +1 more source

A Simple and Effective Method to Concentrate Hepatitis C Virus: Aqueous Two-Phase System Allows Highly Efficient Enrichment of Enveloped Viruses

open access: yesViruses, 2022
To investigate the proliferation cycle of a virus, virus-host interaction, and pathogenesis of a virus, virion particles must be concentrated from the media of virus cell culture or the sera of virus-infected patients.
Heesun Kim   +4 more
doaj   +1 more source

Three method-combination protocol for improving purity of extracellular vesicles [PDF]

open access: yes, 2020
Extracellular vesicles (EVs) are nanosized structures able to carry proteins, lipids and genetic material from one cell to another with critical implications in intercellular communication mechanisms.
Giamas, Georgios   +3 more
core   +1 more source

Comparison of Different Isolation Methods for Plasma-Derived Extracellular Vesicles in Patients with Hyperlipidemia

open access: yesLife, 2022
Extracellular vesicles are commonly found in human body fluids and can reflect current physiological conditions of human body and act as biomarkers of disease.
Ke Zhen   +8 more
doaj   +1 more source

Enrichment of calcifying extracellular vesicles using density-based ultracentrifugation protocol [PDF]

open access: yesJournal of Extracellular Vesicles, 2014
Calcifying extracellular vesicles (EVs) released from cells within atherosclerotic plaques have received increased attention for their role in mediating vascular calcification, a major predictor of cardiovascular morbidity and mortality.
Joshua D. Hutcheson   +6 more
doaj   +1 more source

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