Results 11 to 20 of about 148,661 (188)

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion chromatography

Journal of Extracellular Vesicles, 2021
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in their composition at a cellular and tissue level.
Juan A. Martínez‐Greene, Karina Hernández‐Ortega, Ricardo Quiroz‐Baez, Osbaldo Resendis‐Antonio, Israel Pichardo‐Casas, David A. Sinclair, Bogdan Budnik, Alfredo Hidalgo‐Miranda, Eileen Uribe‐Querol, María del Pilar Ramos‐Godínez, Eduardo Martínez‐Martínez   +10 more
doaj   +1 more source

1Progress, applications, challenges and prospects of protein purification technology

Frontiers in Bioengineering and Biotechnology, 2022
Protein is one of the most important biological macromolecules in life, which plays a vital role in cell growth, development, movement, heredity, reproduction and other life activities.
Miao Du, Zhuru Hou, Ling Liu, Ling Liu, Yan Xuan, Xiaocong Chen, Lei Fan, Zhuoxi Li, Benjin Xu, Benjin Xu   +9 more
doaj   +1 more source

Three method-combination protocol for improving purity of extracellular vesicles [PDF]

, 2020
Extracellular vesicles (EVs) are nanosized structures able to carry proteins, lipids and genetic material from one cell to another with critical implications in intercellular communication mechanisms.
Giamas, Georgios, Kumaran, Anish, Simon, Thomas, Veselu, Diana-Florentina   +3 more
core   +1 more source

Accuracy of 23 Equations for Estimating LDL Cholesterol in a Clinical Laboratory Database of 5,051,467 Patients

Global Heart, 2023
Background: Alternatives to the Friedewald low-density lipoprotein cholesterol (LDL-C) equation have been proposed. Objective: To compare the accuracy of available LDL-C equations with ultracentrifugation measurement.
Christeen Samuel, Jihwan Park, Aparna Sajja, Erin D. Michos, Roger S. Blumenthal, Steven R. Jones, Seth S. Martin   +6 more
doaj   +1 more source

IgA-enhancing effects of membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC15893 [PDF]

, 2018
Immunoglobulin (Ig) A in the mucus of the intestinal tract plays an important role in preventing the invasion of pathogenic microorganisms and regulating the composition of the gut microbiota.
Katakura Yoshio, Kunisawa Jun, Miyoshi Yuki, Nakayama Tomoya, Yamasaki-yashiki Shino, 中山 友哉, 山崎（屋敷） 思乃, 片倉 啓雄   +7 more
core   +2 more sources

Comparative Analysis of Natural and Cytochalasin B-Induced Membrane Vesicles from Tumor Cells and Mesenchymal Stem Cells

Current Issues in Molecular Biology, 2022
To date, there are numerous protocols for the isolation of extracellular vesicles (EVs). Depending on the isolation method, it is possible to obtain vesicles with different characteristics, enriched with specific groups of proteins, DNA and RNA, which ...
Zarema Gilazieva, Daria Chulpanova, Aleksei Ponomarev, Ivan Filin, Ekaterina Garanina, Albert Rizvanov, Valeriya Solovyeva   +6 more
doaj   +1 more source

Protein concentration of synovial fluid in chronic rheumatoid arthritis. Estimation of protein in the synovial fluid of chronic rheumatoid arthritis by gel filtration and paper electrophoresis [PDF]

, 1968
For the purpose to reveal the characteris6cs of the synovial fluid of the chronic rheumatoid arthritis the proteins of the synovial fluid and blood serum have been analysed by employing the methods of electrophoresis, gel filtration on Sephadex G-200 ...
Seno, Takashi
core   +1 more source

Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography

Journal of Extracellular Vesicles, 2017
Studies have suggested that nanoscale extracellular vesicles (EV) in human and bovine milk carry immune modulatory properties which could provide beneficial health effects to infants.
Kristine Blans, Maria S. Hansen, Laila V. Sørensen, Michael L. Hvam, Kenneth A. Howard, Arne Möller, Lars Wiking, Lotte B. Larsen, Jan T. Rasmussen   +8 more
doaj   +1 more source

Proteomic and Biochemical Analysis of Extracellular Vesicles Isolated from Blood Serum of Patients with Melanoma

Separations, 2022
Background: Malignant melanoma is the most serious type of skin cancer with the highest mortality rate. Extracellular vesicles (EVs) have potential as new tumor markers that could be used as diagnostic and prognostic markers for early detection of ...
Kristiina Kurg, Anu Planken, Reet Kurg
doaj   +1 more source

Gated Ethidium- and Bleomycin-Loading in Phage T4 That Is Subsequently Purified Leak-Free

Biophysica, 2022
Chemotherapy-inhibiting tumor cell evolution to drug-resistance is potentially suppressed by using a drug delivery vehicle (DDV) that has gating. Gating would be used to increase tumor-selectivity of delivery of DDV packaged drug.
Philip Serwer, Elena T. Wright
doaj   +1 more source