Results 21 to 30 of about 100,244 (261)

Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography

Journal of Extracellular Vesicles, 2018
As a complex biological fluid, human synovial fluid (SF) presents challenges for extracellular vesicle (EV) enrichment using standard methods. In this study of human SF, a size exclusion chromatography (SEC)-based method of EV enrichment is shown to ...
Andrew D. Foers, Simon Chatfield, Laura F. Dagley, Benjamin J. Scicluna, Andrew I. Webb, Lesley Cheng, Andrew F. Hill, Ian P. Wicks, Ken C. Pang   +8 more
doaj   +1 more source

Production- and Purification-Relevant Properties of Human and Murine Cytomegalovirus

Viruses, 2021
There is a large unmet need for a prophylactic vaccine against human cytomegalovirus (HCMV) to combat the ubiquitous infection that is ongoing with this pathogen.
Sanda Ravlić, Marija Brgles, Lea Hiršl, Stipan Jonjić, Beata Halassy   +4 more
doaj   +1 more source

A Comparison of Blood Plasma Small Extracellular Vesicle Enrichment Strategies for Proteomic Analysis

Proteomes, 2022
Proteomic analysis of small extracellular vesicles (sEVs) poses a significant challenge. A ‘gold-standard’ method for plasma sEV enrichment for downstream proteomic analysis is yet to be established.
Natalie P. Turner, Pevindu Abeysinghe, Keith A. Kwan Cheung, Kanchan Vaswani, Jayden Logan, Pawel Sadowski, Murray D. Mitchell   +6 more
doaj   +1 more source

Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography

Journal of Extracellular Vesicles, 2017
Studies have suggested that nanoscale extracellular vesicles (EV) in human and bovine milk carry immune modulatory properties which could provide beneficial health effects to infants.
Kristine Blans, Maria S. Hansen, Laila V. Sørensen, Michael L. Hvam, Kenneth A. Howard, Arne Möller, Lars Wiking, Lotte B. Larsen, Jan T. Rasmussen   +8 more
doaj   +1 more source

A size-exclusion-based approach for purifying extracellular vesicles from human plasma

Cell Reports: Methods, 2021
Summary: Extracellular vesicles (EVs) are released into blood from multiple organs and carry molecular cargo that facilitates inter-organ communication and an integrated response to physiological and pathological stimuli.
Patrick M. Vanderboom, Surendra Dasari, Gregory N. Ruegsegger, Mark W. Pataky, Fabrice Lucien, Carrie Jo Heppelmann, Ian R. Lanza, K. Sreekumaran Nair   +7 more
doaj   +1 more source

Impact of PEDV infection on the biological characteristics of porcine intestinal exosomes

Frontiers in Microbiology
Porcine epidemic diarrhea (PED) is a highly contagious intestinal infection primarily affecting pigs. It is caused by the porcine epidemic diarrhea virus (PEDV).
Junjie Wu, Langju Su, Guangmiao Ma, Yichen Wang, Yuhang Luo, Saeed EI-Ashram, Reem Atalla Alajmi, Zhili Li   +7 more
doaj   +1 more source

Serum triglyceride and cholesterol concentrations and lipoprotein profiles in dogs with naturally occurring pancreatitis and healthy control dogs

Journal of Veterinary Internal Medicine, 2020
Background Previous studies have reported an association between hyperlipidemia and pancreatitis in dogs, but details of this association remain poorly defined.
Panagiotis G. Xenoulis, Paul J. Cammarata, Rosemary L. Walzem, Jan S. Suchodolski, Jörg M. Steiner   +4 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Raman spectroscopy as a quick tool to assess purity of extracellular vesicle preparations and predict their functionality

Journal of Extracellular Vesicles, 2019
Extracellular vesicles (EVs) from a variety of stem cell sources are believed to harbour regenerative capacity, which may be exploited for therapeutic purposes.
Alice Gualerzi, Sander Alexander Antonius Kooijmans, Stefania Niada, Silvia Picciolini, Anna Teresa Brini, Giovanni Camussi, Marzia Bedoni   +6 more
doaj   +1 more source

Characterization of Plasma-Derived Extracellular Vesicles Isolated by Different Methods: A Comparison Study

Bioengineering, 2019
Extracellular vesicles (EV) are small membrane structures released by cells that act as potent mediators of intercellular communication. The study of EV biology is important, not only to strengthen our knowledge of their physiological roles, but also to ...
Esther Serrano-Pertierra, Myriam Oliveira-Rodríguez, Montserrat Rivas, Pedro Oliva, Javier Villafani, Ana Navarro, M. Carmen Blanco-López, Eva Cernuda-Morollón   +7 more
doaj   +1 more source