Results 31 to 40 of about 151,242 (304)

Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography

Journal of Extracellular Vesicles, 2018
As a complex biological fluid, human synovial fluid (SF) presents challenges for extracellular vesicle (EV) enrichment using standard methods. In this study of human SF, a size exclusion chromatography (SEC)-based method of EV enrichment is shown to ...
Andrew D. Foers, Simon Chatfield, Laura F. Dagley, Benjamin J. Scicluna, Andrew I. Webb, Lesley Cheng, Andrew F. Hill, Ian P. Wicks, Ken C. Pang   +8 more
doaj   +1 more source

A Comparison of Blood Plasma Small Extracellular Vesicle Enrichment Strategies for Proteomic Analysis

Proteomes, 2022
Proteomic analysis of small extracellular vesicles (sEVs) poses a significant challenge. A ‘gold-standard’ method for plasma sEV enrichment for downstream proteomic analysis is yet to be established.
Natalie P. Turner, Pevindu Abeysinghe, Keith A. Kwan Cheung, Kanchan Vaswani, Jayden Logan, Pawel Sadowski, Murray D. Mitchell   +6 more
doaj   +1 more source

Molar mass and solution conformation of branched alpha(1 - 4), alpha(1 - 6) Glucans. Part I: Glycogens in water [PDF]

, 2008
Solution molar masses and conformations of glycogens from different sources (rabbit, oyster, mussel and bovine) were analysed using sedimentation velocity in the analytical ultracentrifuge, size-exclusion chromatography coupled to multi-angle laser light
Ang, S., Harding, S. E., Hill, S. E., Lewis, S., Morris, Gordon, Nobbmann, U., Schafer, B.   +6 more
core   +1 more source

Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma [PDF]

, 2016
BACKGROUND: Growing evidence is showing that metastatic cell populations are able to transfer their characteristics to less malignant cells. Exosomes (EXOs) are membrane vesicles of endocytic origin able to convey their cargo of mRNAs, microRNAs (miRs),
Bellenghi, Maria, Carè, Alessandra, Coscia, Carolina, Errico, Maria Cristina, Felicetti, Federica, Feo, Alessandra De, Pagani, Elena, Pasquini, Luca, Pedini, Francesca, Puglisi, Rossella   +9 more
core   +5 more sources

Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography

Journal of Extracellular Vesicles, 2017
Studies have suggested that nanoscale extracellular vesicles (EV) in human and bovine milk carry immune modulatory properties which could provide beneficial health effects to infants.
Kristine Blans, Maria S. Hansen, Laila V. Sørensen, Michael L. Hvam, Kenneth A. Howard, Arne Möller, Lars Wiking, Lotte B. Larsen, Jan T. Rasmussen   +8 more
doaj   +1 more source

The Solution Structures of Two Human IgG1 Antibodies Show Conformational Stability and Accommodate Their C1q and FcγR Ligands. [PDF]

, 2015
The human IgG1 antibody subclass shows distinct properties compared with the IgG2, IgG3, and IgG4 subclasses and is the most exploited subclass in therapeutic antibodies. It is the most abundant subclass, has a half-life as long as that of IgG2 and IgG4,
Abe, Andrusier, Ashish, Ashton, Boehm, Brekke, Bruhns, Brüggemann, Calmettes, Collins, Dall'Acqua, Dangl, Davies, Deisenhofer, Diebolder, Dorai, Feinstein, Furtado, Gaboriaud, Gar Kay Hui, Garcia de la Torre, García De La Torre, Glatter, Gregory, Guddat, Guex, Hamilton, Harris, Harris, Heenan, Hezareh, Hughes-Jones, Idusogie, Isenman, Iwura, Jayesh Gor, Jefferis, Kilár, Klein, Laue, Lilyestrom, Lucy E. Rayner, Lund, Luo, Manning, Marquart, Michaelsen, Narayanan, Paul A. Dalby, Perkins, Perkins, Perkins, Perkins, Phillips, Pilz, Radaev, Radaev, Rayner, Rayner, Remmele, Richard K. Heenan, Richardson, Roux, Saphire, Saphire, Sarma, Schneider, Schneidman-Duhovny, Schuck, Schuck, Semenyuk, Sondermann, Stephen J. Perkins, Strohl, Sun, Tamm, Tao, Thommesen, Wang, Yang   +79 more
core   +1 more source

A size-exclusion-based approach for purifying extracellular vesicles from human plasma

Cell Reports: Methods, 2021
Summary: Extracellular vesicles (EVs) are released into blood from multiple organs and carry molecular cargo that facilitates inter-organ communication and an integrated response to physiological and pathological stimuli.
Patrick M. Vanderboom, Surendra Dasari, Gregory N. Ruegsegger, Mark W. Pataky, Fabrice Lucien, Carrie Jo Heppelmann, Ian R. Lanza, K. Sreekumaran Nair   +7 more
doaj   +1 more source

Biophysical evidence for intrinsic disorder in the C-terminal tails of the epidermal growth factor receptor (EGFR) and HER3 receptor tyrosine kinases [PDF]

, 2016
The epidermal growth factor receptor (EGFR)/ErbB family of receptor tyrosine kinases includes oncogenes important in the progression of breast and other cancers, and they are targets for many drug development strategies.
Bose, Ron, Keppel, Theodore R, Monsey, John, Murray, Elisa M, Sarpong, Kwabena, Zhu, Jian   +5 more
core   +2 more sources

High-Level Expression of Various Apolipoprotein (a) Isoforms by "Transferrinfection". The Role of Kringle IV Sequences in the Extracellular Association with Low-Density Lipoprotein [PDF]

, 1994
Characterization of the assembly of lipoprotein(a) [Lp(a)] is of fundamental importance to understanding the biosynthesis and metabolism of this atherogenic lipoprotein. Since no established cell lines exist that express Lp(a) or apolipoprotein(a) [apo(a)
Azrolan N., Boerwinkle E., Brunner C., Chiesa G., Chung B. Y., Cotten M., Deutscher S. L., Deutscher S. L., Dieplinger H., Edelberg J. M., Green S., Griffiths G., Guevara J., Jr., Huby T., Ichinose A., Kamboh M. I., Koschinsky M. L., Koschinsky M. L., Koschinsky M. L., Kostner G. M., Kraft H- G., Kraft H. G., Krempler F., Krempler F., Lackner C., Linton M. F., Malgaretti N., McGregor G. R., McLean J. W., Menzel H. J., Phillips M. L., Rader D. J., Sambrook J., Sandholzer C., Scanu A. M., Selden R. F., Sommer A., Stanley P., Trieu V. N., Utermann G., Utermann G., Utermann G., Wagner E., White A. L., Zatloukal K., Zechner R„   +45 more
core   +1 more source

Impact of PEDV infection on the biological characteristics of porcine intestinal exosomes

Frontiers in Microbiology
Porcine epidemic diarrhea (PED) is a highly contagious intestinal infection primarily affecting pigs. It is caused by the porcine epidemic diarrhea virus (PEDV).
Junjie Wu, Langju Su, Guangmiao Ma, Yichen Wang, Yuhang Luo, Saeed EI-Ashram, Reem Atalla Alajmi, Zhili Li   +7 more
doaj   +1 more source