Results 11 to 20 of about 100,244 (261)

Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles?

Biomolecules, 2023
Mineralization-competent cells, including hypertrophic chondrocytes, mature osteoblasts, and osteogenic-differentiated smooth muscle cells secrete media extracellular vesicles (media vesicles) and extracellular vesicles bound to the extracellular matrix (
Saida Mebarek, Rene Buchet, Slawomir Pikula, Agnieszka Strzelecka-Kiliszek, Leyre Brizuela, Giada Corti, Federica Collacchi, Genevieve Anghieri, Andrea Magrini, Pietro Ciancaglini, Jose Luis Millan, Owen Davies, Massimo Bottini   +12 more
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Proteomic and Biochemical Analysis of Extracellular Vesicles Isolated from Blood Serum of Patients with Melanoma

Separations, 2022
Background: Malignant melanoma is the most serious type of skin cancer with the highest mortality rate. Extracellular vesicles (EVs) have potential as new tumor markers that could be used as diagnostic and prognostic markers for early detection of ...
Kristiina Kurg, Anu Planken, Reet Kurg
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Lipoprotein separation in a novel iodixanol density gradient, for composition, density, and phenotype analysis

Journal of Lipid Research, 2008
Separation of lipoproteins by traditional sequential salt density floatation is a prolonged process (∼72 h) with variable recovery, whereas iodixanol-based, self-generating density gradients provide a rapid (∼4 h) alternative.
Michael S. Yee, Darrell V. Pavitt, Tira Tan, Soundararajan Venkatesan, Ian F. Godsland, William Richmond, Desmond G. Johnston   +6 more
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Gated Ethidium- and Bleomycin-Loading in Phage T4 That Is Subsequently Purified Leak-Free

Biophysica, 2022
Chemotherapy-inhibiting tumor cell evolution to drug-resistance is potentially suppressed by using a drug delivery vehicle (DDV) that has gating. Gating would be used to increase tumor-selectivity of delivery of DDV packaged drug.
Philip Serwer, Elena T. Wright
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Comparative Analysis of Natural and Cytochalasin B-Induced Membrane Vesicles from Tumor Cells and Mesenchymal Stem Cells

Current Issues in Molecular Biology, 2022
To date, there are numerous protocols for the isolation of extracellular vesicles (EVs). Depending on the isolation method, it is possible to obtain vesicles with different characteristics, enriched with specific groups of proteins, DNA and RNA, which ...
Zarema Gilazieva, Daria Chulpanova, Aleksei Ponomarev, Ivan Filin, Ekaterina Garanina, Albert Rizvanov, Valeriya Solovyeva   +6 more
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An Integrated Approach for the Efficient Extraction and Solubilization of Rice Microsomal Membrane Proteins for High-Throughput Proteomics

Frontiers in Plant Science, 2021
The preparation of microsomal membrane proteins (MPs) is critically important to microsomal proteomics. To date most research studies have utilized an ultracentrifugation-based approach for the isolation and solubilization of plant MPs.
Truong Van Nguyen, Ravi Gupta, Dicky Annas, Jinmi Yoon, Yu-Jin Kim, Gi Hyun Lee, Jeong Woo Jang, Kang Hyun Park, Randeep Rakwal, Randeep Rakwal, Ki-Hong Jung, Cheol Woo Min, Sun Tae Kim   +12 more
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Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion chromatography

Journal of Extracellular Vesicles, 2021
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in their composition at a cellular and tissue level.
Juan A. Martínez‐Greene, Karina Hernández‐Ortega, Ricardo Quiroz‐Baez, Osbaldo Resendis‐Antonio, Israel Pichardo‐Casas, David A. Sinclair, Bogdan Budnik, Alfredo Hidalgo‐Miranda, Eileen Uribe‐Querol, María del Pilar Ramos‐Godínez, Eduardo Martínez‐Martínez   +10 more
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Optimization of Methods for Determination of the Encapsulation Efficiency of Doxorubicin in the Nanoparticles Based on Poly(lactic-co-glycolic acid) (PLGA)

Разработка и регистрация лекарственных средств, 2020
Introduction. Drug encapsulation efficiency (EE) is the important parameter of the nanoparticle-based drug formulations. Generally, the methods for evaluation of the EE are based on separation of the free and NP-bound fractions of the drug; however ...
T. S. Kovshova, N. S. Osipova, A. S. Fortuna, Yu. V. Ermolenko, O. O. Maksimenko, V. Yu. Balabanyan, S. E. Gelperina   +6 more
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1Progress, applications, challenges and prospects of protein purification technology

Frontiers in Bioengineering and Biotechnology, 2022
Protein is one of the most important biological macromolecules in life, which plays a vital role in cell growth, development, movement, heredity, reproduction and other life activities.
Miao Du, Zhuru Hou, Ling Liu, Ling Liu, Yan Xuan, Xiaocong Chen, Lei Fan, Zhuoxi Li, Benjin Xu, Benjin Xu   +9 more
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The effect of cerebrospinal fluid-derived exosomes on neural differentiation of adipose mesenchymal stem cells in alginate hydrogel scaffold

یافته‌های نوین در علوم زیستی, 2022
Nowadays, researchers have made extensive efforts to find new treatments for nerve damage. Meanwhile, the role of exosomes in cell-cell communication is considered to be a new mechanism. Exosomes can act as suitable differentiating agents.
Mojtaba Cheravi, Javad Baharara, Parichehreh Yaghmaei, Nasim Hayati Roudbari   +3 more
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