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Biophysical studies by ultracentrifugation
Current Opinion in Structural Biology, 2001Advances in data analysis are broadening the applicability of ultracentrifugation to the characterization of macromolecular behavior in complex solution. The direct fitting of sedimentation velocity data to the Lamm equation is emerging as a very powerful means to characterize size distributions, improve the precision of data analysis and increase ...
Tom Laue
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2023
This chapter considers ultracentrifugation, which provides methods for the characterisation of the sizes and shapes of macromolecules and macromolecular assemblies. Ultracentrifugation is used in three ways: velocity sedimentation, equilibrium sedimentation, and density-gradient sedimentation.
Peter Atkins +3 more
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This chapter considers ultracentrifugation, which provides methods for the characterisation of the sizes and shapes of macromolecules and macromolecular assemblies. Ultracentrifugation is used in three ways: velocity sedimentation, equilibrium sedimentation, and density-gradient sedimentation.
Peter Atkins +3 more
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Lipid Exchange by Ultracentrifugation
2016Lipids play an important role in maintaining P-type ATPase structure and function, and often they are crucial for ATPase activity. When the P-type ATPases are in the membrane, they are surrounded by a mix of different lipid species with varying aliphatic chain lengths and saturation, and the complex interplay between the lipids and the P-type ATPases ...
Drachmann, Nikolaj Düring +1 more
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Analytical Ultracentrifugation
Current Protocols in Protein Science, 1996AbstractAnalytical ultracentrifugation is one of the most powerful, though as yet underexploited, techniques available to molecular biology and biochemistry. This overview describes applications for analytical ultracentrifugation along with important considerations relating to experimental design.
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Calibrating analytical ultracentrifuges
European Biophysics Journal, 2021Analytical ultracentrifugation (AUC) is based on the concept of recording and analyzing macroscopic macromolecular redistribution that results from a centrifugal force acting on the mass of suspended macromolecules in solution. Since AUC rests on first principles, it can provide an absolute measurement of macromolecular mass, sedimentation and ...
Huaying Zhao +3 more
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Required ultracentrifugal time for condensing solution by ultracentrifuge
Bioprocess Engineering, 1990Required ultracentrifugal time for condensing a solution by ultracentrifuge with an angle rotor or a swing rotor was estimated by considering the calculated distribution of concentration in an ultracentrifugal tube. From the calculated distribution of the concentration a free boundary between solvent and solution was found in the ultracentrifugal tube ...
N. Shiragami, T. Kajiuchi, K. Ohtomo
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Coping with convection in the ultracentrifuge
Analytical Biochemistry, 1978Abstract If cell orientation and temperature control are good, convective flow in the analytical ultracentrifuge cell occurs due to vibrations transmitted from a faulty drive to the rotor itself. Minor vibrations are usually “ironed out” at high speeds but, at low speeds, (
D B, Millar, J P, Christopher
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