Results 21 to 30 of about 166,094 (382)

An Integrated Approach for the Efficient Extraction and Solubilization of Rice Microsomal Membrane Proteins for High-Throughput Proteomics

Frontiers in Plant Science, 2021
The preparation of microsomal membrane proteins (MPs) is critically important to microsomal proteomics. To date most research studies have utilized an ultracentrifugation-based approach for the isolation and solubilization of plant MPs.
Truong Van Nguyen, Ravi Gupta, Dicky Annas, Jinmi Yoon, Yu-Jin Kim, Gi Hyun Lee, Jeong Woo Jang, Kang Hyun Park, Randeep Rakwal, Randeep Rakwal, Ki-Hong Jung, Cheol Woo Min, Sun Tae Kim   +12 more
doaj   +1 more source

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion chromatography

Journal of Extracellular Vesicles, 2021
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in their composition at a cellular and tissue level.
Juan A. Martínez‐Greene, Karina Hernández‐Ortega, Ricardo Quiroz‐Baez, Osbaldo Resendis‐Antonio, Israel Pichardo‐Casas, David A. Sinclair, Bogdan Budnik, Alfredo Hidalgo‐Miranda, Eileen Uribe‐Querol, María del Pilar Ramos‐Godínez, Eduardo Martínez‐Martínez   +10 more
doaj   +1 more source

1Progress, applications, challenges and prospects of protein purification technology

Frontiers in Bioengineering and Biotechnology, 2022
Protein is one of the most important biological macromolecules in life, which plays a vital role in cell growth, development, movement, heredity, reproduction and other life activities.
Miao Du, Zhuru Hou, Ling Liu, Ling Liu, Yan Xuan, Xiaocong Chen, Lei Fan, Zhuoxi Li, Benjin Xu, Benjin Xu   +9 more
doaj   +1 more source

Accuracy of 23 Equations for Estimating LDL Cholesterol in a Clinical Laboratory Database of 5,051,467 Patients

Global Heart, 2023
Background: Alternatives to the Friedewald low-density lipoprotein cholesterol (LDL-C) equation have been proposed. Objective: To compare the accuracy of available LDL-C equations with ultracentrifugation measurement.
Christeen Samuel, Jihwan Park, Aparna Sajja, Erin D. Michos, Roger S. Blumenthal, Steven R. Jones, Seth S. Martin   +6 more
doaj   +1 more source

IgA-enhancing effects of membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC15893 [PDF]

, 2018
Immunoglobulin (Ig) A in the mucus of the intestinal tract plays an important role in preventing the invasion of pathogenic microorganisms and regulating the composition of the gut microbiota.
Katakura Yoshio, Kunisawa Jun, Miyoshi Yuki, Nakayama Tomoya, Yamasaki-yashiki Shino, 中山 友哉, 山崎（屋敷） 思乃, 片倉 啓雄   +7 more
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Comparative Analysis of Natural and Cytochalasin B-Induced Membrane Vesicles from Tumor Cells and Mesenchymal Stem Cells

Current Issues in Molecular Biology, 2022
To date, there are numerous protocols for the isolation of extracellular vesicles (EVs). Depending on the isolation method, it is possible to obtain vesicles with different characteristics, enriched with specific groups of proteins, DNA and RNA, which ...
Zarema Gilazieva, Daria Chulpanova, Aleksei Ponomarev, Ivan Filin, Ekaterina Garanina, Albert Rizvanov, Valeriya Solovyeva   +6 more
doaj   +1 more source

Protein concentration of synovial fluid in chronic rheumatoid arthritis. Estimation of protein in the synovial fluid of chronic rheumatoid arthritis by gel filtration and paper electrophoresis [PDF]

, 1968
For the purpose to reveal the characteris6cs of the synovial fluid of the chronic rheumatoid arthritis the proteins of the synovial fluid and blood serum have been analysed by employing the methods of electrophoresis, gel filtration on Sephadex G-200 ...
Seno, Takashi
core   +1 more source

Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography

Journal of Extracellular Vesicles, 2017
Studies have suggested that nanoscale extracellular vesicles (EV) in human and bovine milk carry immune modulatory properties which could provide beneficial health effects to infants.
Kristine Blans, Maria S. Hansen, Laila V. Sørensen, Michael L. Hvam, Kenneth A. Howard, Arne Möller, Lars Wiking, Lotte B. Larsen, Jan T. Rasmussen   +8 more
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Proteomic and Biochemical Analysis of Extracellular Vesicles Isolated from Blood Serum of Patients with Melanoma

Separations, 2022
Background: Malignant melanoma is the most serious type of skin cancer with the highest mortality rate. Extracellular vesicles (EVs) have potential as new tumor markers that could be used as diagnostic and prognostic markers for early detection of ...
Kristiina Kurg, Anu Planken, Reet Kurg
doaj   +1 more source

Cholesterol nucleation time in gallbladder bile of patients with solitary or multiple cholesterol gallstones [PDF]

, 1992
Patients with multiple cholesterol gallbladder stones have been found to be at a higher risk for the recurrence of gallstones after successful nonsurgical treatment than those with a solitary stone.
Abell, Admirand, Carey, Carey, Christoph von Ritter, Dieter Jüngst, Ekkehard Pratschke, Fiske, Gollish, Groen, Gustav Paumgartner, Holan, Lanzini, O'Donnel, Pattinson, Pomeranz, Sackmann, Schriever, Sedaghat, Shiffman, Smallwood, Spengler, Talalay, Tera, Thomas Lang, Van Erpecum, Villanova, Whiting   +27 more
core   +1 more source