Results 31 to 40 of about 166,094 (382)

Gated Ethidium- and Bleomycin-Loading in Phage T4 That Is Subsequently Purified Leak-Free

Biophysica, 2022
Chemotherapy-inhibiting tumor cell evolution to drug-resistance is potentially suppressed by using a drug delivery vehicle (DDV) that has gating. Gating would be used to increase tumor-selectivity of delivery of DDV packaged drug.
Philip Serwer, Elena T. Wright
doaj   +1 more source

Complexity of the microRNA transcriptome of cow milk and milk-derived extracellular vesicles isolated via differential ultracentrifugation.

Journal of Dairy Science, 2020
MicroRNA (miRNA) are small gene-regulatory noncoding RNA that are highly enriched in cow milk. They are encapsulated in different extracellular vesicle (EV) subsets that protect them from the extracellular milieu and the harsh conditions of the ...
A. Benmoussa, Jonathan Laugier, C. Beauparlant, Marine Lambert, Arnaud Droit, P. Provost   +5 more
semanticscholar   +1 more source

Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography

Journal of Extracellular Vesicles, 2018
As a complex biological fluid, human synovial fluid (SF) presents challenges for extracellular vesicle (EV) enrichment using standard methods. In this study of human SF, a size exclusion chromatography (SEC)-based method of EV enrichment is shown to ...
Andrew D. Foers, Simon Chatfield, Laura F. Dagley, Benjamin J. Scicluna, Andrew I. Webb, Lesley Cheng, Andrew F. Hill, Ian P. Wicks, Ken C. Pang   +8 more
doaj   +1 more source

Do Media Extracellular Vesicles and Extracellular Vesicles Bound to the Extracellular Matrix Represent Distinct Types of Vesicles?

Biomolecules, 2023
Mineralization-competent cells, including hypertrophic chondrocytes, mature osteoblasts, and osteogenic-differentiated smooth muscle cells secrete media extracellular vesicles (media vesicles) and extracellular vesicles bound to the extracellular matrix (
Saida Mebarek, Rene Buchet, Slawomir Pikula, Agnieszka Strzelecka-Kiliszek, Leyre Brizuela, Giada Corti, Federica Collacchi, Genevieve Anghieri, Andrea Magrini, Pietro Ciancaglini, Jose Luis Millan, Owen Davies, Massimo Bottini   +12 more
doaj   +1 more source

Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK–TssFG subcomplex [PDF]

, 2014
Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However, the mechanism of the T6SS is incompletely understood. In the present study, we investigated a conserved component of
Alan R. Prescott, Aschtgen, Aschtgen, Aschtgen, Aschtgen, Ballister, Basler, Basler, Bonemann, Bonemann, Boyer, Burtnick, Casabona, Casabona, Cascales, Coulthurst, Dong, English, Felisberto-Rodrigues, Francesca R. Cianfanelli, Fritsch, Gerlach, Grant English, Holland, Hood, Jack, Kaniga, Kapitein, Kapitein, Laue, Leiman, Leiman, Lossi, Ma, Ma, Ma, MacIntyre, Mougous, Murdoch, Olwyn Byron, Pell, Potvin, Pukatzki, Rao, Rickman, Russell, Russell, Sambrook, Sarah J. Coulthurst, Schell, Schuck, Schwarz, Shneider, Silverman, Srikannathasan, Suarez, Vistica, Whitney, Zheng, Zheng, Zoued   +60 more
core   +2 more sources

A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents

PLoS ONE, 2017
Exosomes play a role in cell-to-cell signaling and serve as possible biomarkers. Isolating exosomes with reliable quality and substantial concentration is a major challenge.
Inas Helwa, Jingwen Cai, M. Drewry, Arthur D. Zimmerman, M. Dinkins, M. L. Khaled, Mutsa P. Seremwe, W. Dismuke, E. Bieberich, W. Stamer, M. Hamrick, Yutao Liu   +11 more
semanticscholar   +1 more source

Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles

Biological Procedures Online, 2020
Background Circulating small extracellular vesicles (sEVs) and its associated proteins are of great interest in the early detection of many diseases. However, there is no gold standard for plasma sEVs isolation, especially for proteomic profiling which ...
Rui Wei, Libo Zhao, Guanyi Kong, Xiang Liu, Shengtao Zhu, Shutian Zhang, L. Min   +6 more
semanticscholar   +1 more source

The effect of cerebrospinal fluid-derived exosomes on neural differentiation of adipose mesenchymal stem cells in alginate hydrogel scaffold

یافته‌های نوین در علوم زیستی, 2022
Nowadays, researchers have made extensive efforts to find new treatments for nerve damage. Meanwhile, the role of exosomes in cell-cell communication is considered to be a new mechanism. Exosomes can act as suitable differentiating agents.
Mojtaba Cheravi, Javad Baharara, Parichehreh Yaghmaei, Nasim Hayati Roudbari   +3 more
doaj  

Analytical ultracentrifugation of colloids

Nanoscale, 2010
In this contribution the use of Analytical Ultracentrifugation (AUC) for the modern analysis of colloids is reviewed. Since AUC is a fractionation technique, distributions of the sedimentation coefficient, particle size and shape, molar mass and density can be obtained for particle sizes spanning the entire colloidal range.
Planken, Karel L., Cölfen, Helmut
openaire   +4 more sources

Synthesis of empty bacterial microcompartments, directed organelle protein incorporation, and evidence of filament-associated organelle movement [PDF]

, 2010
Compartmentalization is an important process, since it allows the segregation of metabolic activities and, in the era of synthetic biology, represents an important tool by which defined microenvironments can be created for specific metabolic functions ...
Bhella, D., Frank, S., Liang, M., Mulvihill, D.P., Parsons, J.B., Prentice, M.B., Warren, M.J.   +6 more
core   +2 more sources