Results 41 to 50 of about 166,094 (382)

Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential

Journal of Extracellular Vesicles, 2018
Interest in small extracellular vesicles (sEVs) as functional carriers of proteins and nucleic acids is growing continuously. There are large numbers of sEVs in the blood, but lack of standardised methods for sEV isolation greatly limits our ability to ...
K. Takov, D. Yellon, S. Davidson
semanticscholar   +1 more source

FolX from Pseudomonas aeruginosa is octameric in both crystal and solution [PDF]

, 2012
FolX encodes an epimerase that forms one step of the tetrahydrofolate biosynthetic pathway, which is of interest as it is an established target for important drugs.
Achari, Andrew J. Roe, Barton, Bond, Davis, Emsley, Evans, Fleischmann, Franke, Gabrielsen, Haussmann, Katherine S.H. Beckham, Kozin, Krissinel, Laue, Leslie, Long, Mads Gabrielsen, Olwyn Byron, Ploom, Pribat, Rai, Richard J. Cogdell, Schuck, Solovyova, Spitzy, Svergun, Volkov, Wang   +28 more
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Serum triglyceride and cholesterol concentrations and lipoprotein profiles in dogs with naturally occurring pancreatitis and healthy control dogs

Journal of Veterinary Internal Medicine, 2020
Background Previous studies have reported an association between hyperlipidemia and pancreatitis in dogs, but details of this association remain poorly defined.
Panagiotis G. Xenoulis, Paul J. Cammarata, Rosemary L. Walzem, Jan S. Suchodolski, Jörg M. Steiner   +4 more
doaj   +1 more source

Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics

Nature Communications, 2019
The study of protein localisation has greatly benefited from high-throughput methods utilising cellular fractionation and proteomic profiling. Hyperplexed Localisation of Organelle Proteins by Isotope Tagging (hyperLOPIT) is a well-established method in ...
Aikaterini Geladaki, Nina Kočevar Britovšek, L. Breckels, Tom S. Smith, Owen L. Vennard, C. Mulvey, Oliver M. Crook, L. Gatto, K. Lilley   +8 more
semanticscholar   +1 more source

Polarized cell motility induces hydrogen peroxide to inhibit cofilin via cysteine oxidation [PDF]

, 2015
Mesenchymal cell motility is driven by polarized actin polymerization [1]. Signals at the leading edge recruit actin polymerization machinery to promote membrane protrusion, while matrix adhesion generates tractive force to propel forward movement.
Anderson, Kurt I., Cameron, Jenifer M., Chim, Ya Hua, Eaton, Philip, Gabrielsen, Mads, McGhee, Ewan J., Munro, June, Olson, Michael F., Sumpton, David, Yin, Huabing   +9 more
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An improvised one-step sucrose cushion ultracentrifugation method for exosome isolation from culture supernatants of mesenchymal stem cells

Stem cell research & therapeutics, 2018
Exosomes are nanovesicles (30–120 nm) of endosomal origin. These exosomes contain various functional proteins and RNAs that could be used for therapeutic purposes.
Suchi Gupta, Sonali Rawat, V. Arora, S. K. Kottarath, A. Dinda, P. Vaishnav, B. Nayak, S. Mohanty   +7 more
semanticscholar   +1 more source

Ultracentrifugation for ultrafine nanodiamond fractionation

, 2017
In this paper we propose a method for ultrafine fractionation of nanodiamonds using the differential centrifugation in the fields up to 215000g. The developed protocols yield 4-6 nm fraction giving main contribution to the light scattering intensity. The
Besedina, N. A., Eidelman, E. D., Kirilenko, D. A., Koniakhin, S. V., Shvidchenko, A. V.   +4 more
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A simple method to assess the oxidative susceptibility of low density lipoproteins [PDF]

, 2001
BACKGROUND: Oxidative modification of low density lipoproteins (LDL) is recognized as one of the major processes involved in atherogenesis. The in vitro standardized measurement of LDL oxidative susceptibility could thus be of clinical significance.
A Lavy, B Guerci, B Wallin, C Coudray, CJ Schwartz, D Steinberg, D Steinbergt, H Esterbauer, H Esterbauer, H Ohkawa, JA Buege, JM Brun, M El-Saadani, M Taus, MA Moss, MAQ Arshad, MM Bradford, N Rifai, PN Demacker, TJ Lyons   +19 more
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The histone H3K4 demethylase JARID1A directly interacts with haematopoietic transcription factor GATA1 in erythroid cells through its second PHD domain [PDF]

, 2020
Chromatin remodelling and transcription factors play important roles in lineage commitment and development through control of gene expression. Activation of selected lineage-specific genes and repression of alternative lineage-affiliated genes results in
Biasutto, Antonio J, Gilbert, Robert C G, Karia, Dimple, Mancini, Erika J, Porcher, Catherine   +4 more
core   +1 more source

Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma

Frontiers in Physiology, 2018
Background: Extracellular vesicles (EVs) (isolated from blood plasma) are currently being extensively researched, both as biomarkers and for their therapeutic possibilities.
Zsófia Onódi, Csilla Pelyhe, Csilla Terézia Nagy, G. Brenner, L. Almási, Á. Kittel, Mateja Manček-Keber, P. Ferdinandy, E. Buzás, Z. Giricz   +9 more
semanticscholar   +1 more source