Results 181 to 190 of about 12,810 (206)
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Trypsin purification by affinity binding to small unilamellar liposomes
Biotechnology and Bioengineering, 1990AbstractA novel protein purification process using affinity‐ligand‐modified liposomes and membrane ultrafiltration is described. The feasibility of the process was tested using trypsin as the model protein and p‐aminobenzamidine (PAB) as the affinity ligand for trypsin.
J D, Powers +2 more
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Covalent Immobilization of Unilamellar Liposomes in Gel Beads for Chromatography
Analytical Biochemistry, 1999For immobilized (proteo)liposome chromatography, unilamellar liposomes were covalently bound within gel beads that had been activated by CNBr, N-hydroxysuccinimide, tresyl, or chloroformate. Liposomes composed of phosphatidylcholine (PC) and 2 mol% of amino-containing lipid (phosphatidylethanolamine-caproylamine) were immobilized in the activated gels ...
Q, Yang +4 more
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Radiation-Induced Changes in Permeability in Unilamellar Phospholipid Liposomes
Radiation Research, 2002Gamma-radiation-induced oxidative damage in unilamellar dipalmitoylphosphatidylcholine liposomes was investigated using a fluorescence technique. Liposomal changes in permeability induced by gamma radiation were monitored by measuring the leakage of pre-encapsulated 6-carboxyfluorescein, and alterations in lipid bilayer fluidity were determined by 1,6 ...
Dipti, Marathe, K P, Mishra
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Efficient clodronate entrapment within multilamellar and unilamellar liposomes
Journal of Pharmacological and Toxicological Methods, 1992Clodronate (dichloromethylene bisphosphonate) encapsulated within liposomes and administered intravenously eliminates resident macrophages within the liver and spleen. Macrophage depletion in the rat requires 20 mg of the encapsulated drug, and so far this has only been achieved using large multilamellar vesicles (MLV).
W G, Love, J P, Camilleri, B D, Williams
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Solution effects on the thermotropic phase transition of unilamellar liposomes
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1991We have investigated the effect of two monosaccharides, glucose and fructose, and two disaccharides, sucrose and trehalose, on the thermotropic phase transition of unilamellar extruded vesicles of DPPC. All the sugars investigated raise the main transition temperature (Tm) of some fraction of the lipid, but there are differences between the effect of ...
L M, Crowe, J H, Crowe
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Noncompetitive Immunoassays Using Bifunmctional Unilamellar Vesicles or Liposomes
Biotechnology Progress, 1995AbstractSmall unilamellar vesicles (SUVs) functionalized with an enzyme label and with specific ligands for biological molecules are useful as signal enhancement vehicles in the development of enzyme‐linked immunoadsorbent assays and other biosensor applications.
A K, Singh +2 more
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Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes
2003The carbohydrate residues of glycosphingolipids were implicated in many biologic processes such as cell-to-cell interactions; and as receptors for some viruses, bacterial and plant toxins, hormones, and so forth, and invariably for all the lectins (1). However, their receptor functions remained poorly defined for a long time as they form micelles even ...
Satish, PR, Surolia, Avadhesha
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Polyelectrolyte-Coated Unilamellar Nanometer-Sized Magnetic Liposomes
Langmuir, 2009Superparamagnetic nanoparticles were encapsulated in liposomes prior to the stepwise adsorption of polyelectrolytes of opposite charges commonly known as the layer-by-layer (LbL) technique. Magnetic fields allow a fast separation of coated liposomes from unbound polyelectrolytes. The coated particles were characterized by dynamic light scattering (DLS),
Pereira da Silva Gomes, Joana Filipa +5 more
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Cholesterol stimulation of penetration of unilamellar liposomes by hydrophobic compounds
The Journal of Membrane Biology, 1977The incorporation of cholesterol into unilamellar liposomes greatly increased the transmembranous movement of hydrophobic ionophores such as nigericin. In reconstituted liposomes containing rhodopsin as the only protein, the presence of cholesterol lowers by 10-fold or more the amount of negericin required to eliminate the light-driven proton gradient.
E F, LaBelle, E, Racker
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Encapsulation of doxorubicin in thermosensitive small unilamellar vesicle liposomes
European Journal of Cancer and Clinical Oncology, 1991The optimisation of the formulation of thermosensitive, doxorubicin-containing small unilamellar liposomes is described. The liposomes were first strictly defined in terms of size distribution and size stability and a quality level was defined. The suspension contained more than 95% vesicles with a maximal diameter of 50 nm and kept this level for a ...
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