Results 181 to 190 of about 14,273 (226)
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Making Unilamellar Liposomes Using Focused Ultrasound
Langmuir, 2011Several techniques are available for making large unilamellar vesicles (LUV) with an average diameter of approximately 100 nm, widely employed as model biomembranes as well as vehicles for drug delivery. Here we describe the use of adaptive focused ultrasound (AFU) for the preparation of LUV from multilamellar vesicles (MLV) and studied the effects of ...
Tejera-Garcia, Roberto +5 more
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The effects of charge and size on the interaction of unilamellar liposomes with macrophages
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1984The effect of the positive surface charge of unilamellar liposomes on the kinetics of their interaction with rat peritoneal macrophages was investigated using three sizes of liposomes: small unilamellar vesicles (approx. 25 nm diameter), prepared by sonication, and large unilamellar vesicles (100 nm and 160 nm diameter), prepared by the Lipoprep ...
R A, Schwendener +2 more
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Trypsin purification by affinity binding to small unilamellar liposomes
Biotechnology and Bioengineering, 1990AbstractA novel protein purification process using affinity‐ligand‐modified liposomes and membrane ultrafiltration is described. The feasibility of the process was tested using trypsin as the model protein and p‐aminobenzamidine (PAB) as the affinity ligand for trypsin.
J D, Powers +2 more
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Predicting hydrophilic drug encapsulation inside unilamellar liposomes
International Journal of Pharmaceutics, 2012A mathematical model has been developed to predict the encapsulation efficiency of hydrophilic drugs in unilamellar liposomes, and will be useful in formulation development to rapidly achieve optimized formulations. This model can also be used to compare drug encapsulation efficiencies of liposomes prepared via different methods, and will assist in the
Xiaoming, Xu +2 more
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Covalent Immobilization of Unilamellar Liposomes in Gel Beads for Chromatography
Analytical Biochemistry, 1999For immobilized (proteo)liposome chromatography, unilamellar liposomes were covalently bound within gel beads that had been activated by CNBr, N-hydroxysuccinimide, tresyl, or chloroformate. Liposomes composed of phosphatidylcholine (PC) and 2 mol% of amino-containing lipid (phosphatidylethanolamine-caproylamine) were immobilized in the activated gels ...
Q, Yang +4 more
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Radiation-Induced Changes in Permeability in Unilamellar Phospholipid Liposomes
Radiation Research, 2002Gamma-radiation-induced oxidative damage in unilamellar dipalmitoylphosphatidylcholine liposomes was investigated using a fluorescence technique. Liposomal changes in permeability induced by gamma radiation were monitored by measuring the leakage of pre-encapsulated 6-carboxyfluorescein, and alterations in lipid bilayer fluidity were determined by 1,6 ...
Dipti, Marathe, K P, Mishra
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Efficient clodronate entrapment within multilamellar and unilamellar liposomes
Journal of Pharmacological and Toxicological Methods, 1992Clodronate (dichloromethylene bisphosphonate) encapsulated within liposomes and administered intravenously eliminates resident macrophages within the liver and spleen. Macrophage depletion in the rat requires 20 mg of the encapsulated drug, and so far this has only been achieved using large multilamellar vesicles (MLV).
W G, Love, J P, Camilleri, B D, Williams
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Solution effects on the thermotropic phase transition of unilamellar liposomes
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1991We have investigated the effect of two monosaccharides, glucose and fructose, and two disaccharides, sucrose and trehalose, on the thermotropic phase transition of unilamellar extruded vesicles of DPPC. All the sugars investigated raise the main transition temperature (Tm) of some fraction of the lipid, but there are differences between the effect of ...
L M, Crowe, J H, Crowe
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Noncompetitive Immunoassays Using Bifunmctional Unilamellar Vesicles or Liposomes
Biotechnology Progress, 1995AbstractSmall unilamellar vesicles (SUVs) functionalized with an enzyme label and with specific ligands for biological molecules are useful as signal enhancement vehicles in the development of enzyme‐linked immunoadsorbent assays and other biosensor applications.
A K, Singh +2 more
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Preparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar Liposomes
2003The carbohydrate residues of glycosphingolipids were implicated in many biologic processes such as cell-to-cell interactions; and as receptors for some viruses, bacterial and plant toxins, hormones, and so forth, and invariably for all the lectins (1). However, their receptor functions remained poorly defined for a long time as they form micelles even ...
Satish, PR, Surolia, Avadhesha
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