Results 191 to 200 of about 12,490 (237)
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Noncompetitive Immunoassays Using Bifunmctional Unilamellar Vesicles or Liposomes
Biotechnology Progress, 1995AbstractSmall unilamellar vesicles (SUVs) functionalized with an enzyme label and with specific ligands for biological molecules are useful as signal enhancement vehicles in the development of enzyme‐linked immunoadsorbent assays and other biosensor applications.
A K, Singh +2 more
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Biochimica et Biophysica Acta (BBA) - Biomembranes, 1986
We investigated the intrahepatic distribution of small unilamellar liposomes injected intravenously into rats at a dose of 0.10 mmol of lipid per kg body weight. Sonicated liposomes consisting of cholesterol/sphingomyelin (1:1), (A); cholesterol/egg phosphatidylcholine (1:1), (B); cholesterol/sphingomyelin/phosphatidylserine (5:4:1), (C) or cholesterol/
SPANJER, HH +4 more
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We investigated the intrahepatic distribution of small unilamellar liposomes injected intravenously into rats at a dose of 0.10 mmol of lipid per kg body weight. Sonicated liposomes consisting of cholesterol/sphingomyelin (1:1), (A); cholesterol/egg phosphatidylcholine (1:1), (B); cholesterol/sphingomyelin/phosphatidylserine (5:4:1), (C) or cholesterol/
SPANJER, HH +4 more
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Trypsin purification by affinity binding to small unilamellar liposomes
Biotechnology and Bioengineering, 1990AbstractA novel protein purification process using affinity‐ligand‐modified liposomes and membrane ultrafiltration is described. The feasibility of the process was tested using trypsin as the model protein and p‐aminobenzamidine (PAB) as the affinity ligand for trypsin.
J D, Powers +2 more
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Radiation-Induced Changes in Permeability in Unilamellar Phospholipid Liposomes
Radiation Research, 2002Gamma-radiation-induced oxidative damage in unilamellar dipalmitoylphosphatidylcholine liposomes was investigated using a fluorescence technique. Liposomal changes in permeability induced by gamma radiation were monitored by measuring the leakage of pre-encapsulated 6-carboxyfluorescein, and alterations in lipid bilayer fluidity were determined by 1,6 ...
Dipti, Marathe, K P, Mishra
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Reduction of doxyl stearates by ascorbate in unilamellar liposomes
Archives of Biochemistry and Biophysics, 1990The effects of the position in the membrane of the doxyl group on the rate of reduction by external ascorbate were studied in large unilamellar liposomes. The key factor increasing the rate of reduction was the degree of partitioning of the nitroxide into the aqueous phase; the doxyl group's proximity to the surface of the membrane was not a major ...
M, Sentjurc, G, Bacic, H M, Swartz
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Preparation of Small Unilamellar Vesicle Liposomes Using Detergent Dialysis Method
2023Small unilamellar liposomes are commonly used as model biomembranes and carriers for drug and gene delivery. Although methods which employ mechanical forces or organic solvents can be used to reduce the liposome size and the number of lipid bilayers, they are not suitable options when the purpose is to incorporate biologically active proteins into ...
Qingyue, Zhong, Hongwei, Zhang
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Solubilization of Large Unilamellar Liposomes by Alkyl Glycosides
Journal of Colloid and Interface Science, 1997The study of biological membranes involves the dissociation, characterization and re-assembly of membrane components. The most important aspect of this approach is the use of an appropriate surfactant as solubilizing agent. In this work the solubilization of large unilamellar liposomes by alkyl glycosides has been studied.
, Ribosa +3 more
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Solution effects on the thermotropic phase transition of unilamellar liposomes
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1991We have investigated the effect of two monosaccharides, glucose and fructose, and two disaccharides, sucrose and trehalose, on the thermotropic phase transition of unilamellar extruded vesicles of DPPC. All the sugars investigated raise the main transition temperature (Tm) of some fraction of the lipid, but there are differences between the effect of ...
L M, Crowe, J H, Crowe
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Unilamellar liposomes covalently coupled on silica gel for liquid chromatography
Analytical and Bioanalytical Chemistry, 2003Silica gel was used as a support for the covalent coupling of liposomes, which could overcome drawbacks of soft gel beads in column efficiency and separation speed. The influences of the concentration of added dimethylaminopyridine and reaction time on the chloroformate activation reaction of silica gel were investigated.
Xiqin, Mao +4 more
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Factors Affecting Responsivity of Unilamellar Liposomes to 20 kHz Ultrasound
Langmuir, 2004Ultrasound is commonly used in the preparation of unilamellar liposome dispersions and is often considered for cell membrane disruption for drug delivery or DNA transfection applications. To better understand the physical and chemical properties of lipid membranes that render them susceptible to ultrasonic permeabilization, the roles of temperature ...
Hung-Yin, Lin, James L, Thomas
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