Results 21 to 30 of about 1,161,651 (341)

Genetic Code Engineering by Natural and Unnatural Base Pair Systems for the Site-Specific Incorporation of Non-Standard Amino Acids Into Proteins

Frontiers in Molecular Biosciences, 2022
Amino acid sequences of proteins are encoded in nucleic acids composed of four letters, A, G, C, and T(U). However, this four-letter alphabet coding system limits further functionalities of proteins by the twenty letters of amino acids.
Michiko Kimoto, Ichiro Hirao
doaj   +1 more source

Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids

Nature Communications, 2023
The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant ...
Yunjun Pan, Guobang Li, Ruxin Liu, Jiawei Guo, Yunjie Liu, Mingyu Liu, Xingwang Zhang, Luping Chi, Kangwei Xu, Ruibo Wu, Yuzhong Zhang, Yuezhong Li, Xiang Gao, Shengying Li   +13 more
doaj   +1 more source

Genetically incorporated crosslinkers reveal NleE attenuates host autophagy dependent on PSMD10

eLife, 2021
Autophagy acts as a pivotal innate immune response against infection. Some virulence effectors subvert the host autophagic machinery to escape the surveillance of autophagy.
Jingxiang Li, Shupan Guo, Fangni Chai, Qi Sun, Pan Li, Li Gao, Lunzhi Dai, Xiaoxiao Ouyang, Zhihui Zhou, Li Zhou, Wei Cheng, Shiqian Qi, Kefeng Lu, Haiyan Ren   +13 more
doaj   +1 more source

Discovery of Staircase Chirality through the Design of Unnatural Amino Acid Derivatives. [PDF]

Research (Wash D C)
Chirality has garnered significant attention in the scientific community since its discovery by Louis Pasteur over a century ago. It has been showing a profound impact on chemical, biomedical, and materials sciences. Significant progress has been made in
Rahman AU, Wang Y, Xu T, Reddy KD, Jin S, Yan JX, Yuan Q, Unruh D, Liang R, Li G.   +9 more
europepmc   +2 more sources

Substrates incorporating unnatural amino acids are separately detectable for chymotrypsin and subtilisin with similar specificity

Results in Chemistry, 2023
Protease specificity depends on the three-dimensional structure and sequence of the protease, and each protease plays a different role. Chymotrypsin and subtilisin have similar specificity for recognizing aromatic and hydrophobic amino acid residues ...
Yuuki Yamawaki, Tamaki Kato
doaj   +1 more source

Genetic incorporation of unnatural amino acids into proteins in Mycobacterium tuberculosis. [PDF]

PLoS ONE, 2010
New tools are needed to study the intracellular pathogen Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), to facilitate new drug discovery and vaccine development.
Feng Wang, Scott Robbins, Jiantao Guo, Weijun Shen, Peter G Schultz   +4 more
doaj   +1 more source

Bicyclic Pyrrolidine-Isoxazoline γ Amino Acid: A Constrained Scaffold for Stabilizing α-Turn Conformation in Isolated Peptides

Frontiers in Chemistry, 2019
Unnatural amino acids have tremendously expanded the folding possibilities of peptides and peptide mimics. While α,α-disubstituted and β-amino acids are widely studied, γ-derivatives have been less exploited.
Francesco Oliva, Raffaella Bucci, Lucia Tamborini, Stefano Pieraccini, Andrea Pinto, Sara Pellegrino   +5 more
doaj   +1 more source

Site-Specific Antibody Conjugation with Payloads beyond Cytotoxins

Molecules, 2023
As antibody–drug conjugates have become a very important modality for cancer therapy, many site-specific conjugation approaches have been developed for generating homogenous molecules.
Qun Zhou
doaj   +1 more source

Activation of Prp28 ATPase by phosphorylated Npl3 at a critical step of spliceosome remodeling

Nature Communications, 2021
Yeast helicase Prp28 promotes the first step of spliceosome remodeling. By placing a photoactivatable unnatural amino acid in Prp28, the authors capture Prp28 in action revealing its dynamic interactions and cofactor Npl3.
Fu-Lung Yeh, Shang-Lin Chang, Golam Rizvee Ahmed, Hsin-I Liu, Luh Tung, Chung-Shu Yeh, Leah Stands Lanier, Corina Maeder, Che-Min Lin, Shu-Chun Tsai, Wan-Yi Hsiao, Wei-Hau Chang, Tien-Hsien Chang   +12 more
doaj   +1 more source

Optimizing non-natural protein function with directed evolution [PDF]

, 2011
Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit ...
Arnold, Frances H., Brustad, Eric M.
core   +1 more source