Results 251 to 260 of about 33,743 (304)
Some of the next articles are maybe not open access.
Genetic incorporation of recycled unnatural amino acids
Amino Acids, 2015The genetic incorporation of unnatural amino acids (UAAs) into proteins has been a useful tool for protein engineering. However, most UAAs are expensive, and the method requires a high concentration of UAAs, which has been a drawback of the technology, especially for large-scale applications.
Wooseok, Ko +3 more
openaire +2 more sources
Site-specific mutagenesis with unnatural amino acids
Trends in Biochemical Sciences, 1989The incorporation of unnatural amino acids into proteins by site-specific mutagenesis provides a valuable new methodology for the generation of novel proteins that possess unique structural and functional features.
S J, Anthony-Cahill +4 more
openaire +2 more sources
Microwave-assisted synthesis of unnatural amino acids
Bioorganic & Medicinal Chemistry Letters, 2008Microwave irradiation has been proven to be a useful tool in the rapid assembly of racemic unnatural amino acids in only two steps. Additional benefits of this methodology are the commercial availability of the inexpensive starting materials and the high yields and high purities of the final amino acid products.
Douglas D, Young +2 more
openaire +2 more sources
An unnatural amino acid that mimics phosphotyrosine
Chemical Communications, 2010By replacing the phosphate group in pTyr with an isoxazole carboxylic acid, a novel unnatural amino acid has been successfully synthesized. Subsequently, its incorporation into a known PPI (protein-protein interaction) inhibitor of STAT3 protein (ISS 610) generated I2 which showed reasonable anti-STAT3 activity in both fluorescence polarization and ...
Ge, J., Wu, H., Yao, S.Q.
openaire +2 more sources
Probing Protein Stability with Unnatural Amino Acids
Science, 1992Unnatural amino acid mutagenesis, in combination with molecular modeling and simulation techniques, was used to probe the effect of side chain structure on protein stability. Specific replacements at position 133 in T4 lysozyme included (i) leucine (wt), norvaline, ethylglycine, and alanine to measure the cost of stepwise removal of methyl
D, Mendel +5 more
openaire +2 more sources
Unnatural amino acid mutagenesis-based enzyme engineering
Trends in Biotechnology, 2015Traditional enzyme engineering relies on substituting one amino acid by one of the other 19 natural amino acids to change the functional properties of an enzyme. However, incorporation of unnatural amino acids (UAAs) has been harnessed to engineer efficient enzymes for biocatalysis.
Yuvaraj, Ravikumar +4 more
openaire +2 more sources
Unnatural amino acids: production and biotechnological potential
World Journal of Microbiology and Biotechnology, 2019Unnatural amino acids (UAAs) are valuable building blocks in the manufacture of a wide range of pharmaceuticals. UAAs exhibit biological activity as free acids and they can be incorporated into linear or cyclic peptides with biological activity.
Tanja Narancic +2 more
openaire +2 more sources
Unnatural Amino Acids in Novel Antibody Conjugates
Future Medicinal Chemistry, 2014Antibody-drug conjugates are an important and emerging drug class for the treatment of cancer. Recent evidence strongly suggests that site-specific drug conjugation results in a homogenous population of molecules with more favorable activity and pharmacokinetic properties than randomly conjugated antibodies.
Trevor J, Hallam, Vaughn V, Smider
openaire +2 more sources
ChemInform Abstract: Unnatural Amino Acids
ChemInform, 2008AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
openaire +1 more source
Regulation of transcription by unnatural amino acids
Nature Biotechnology, 2011Small-molecule regulation of gene expression is intrinsic to cellular function and indispensable to the construction of new biological sensing, control and expression systems. However, there are currently only a handful of strategies for engineering such regulatory components and fewer still that can give rise to an arbitrarily large set of inducible ...
Chang C, Liu +3 more
openaire +2 more sources