Results 101 to 110 of about 22,001 (241)

Host‐feeding preferences of Culex pipiens and its potential significance for flavivirus transmission in the Camargue, France

Medical and Veterinary Entomology, EarlyView.
We identified Culex pipiens bloodmeals at the species level in natural, agricultural and peri‐urban habitats and calculated host preferences based on forage ratios. This analysis showed Passeriformes, and in particular Columba livia, Passer montanus and Turdus sp., to be the preferred Cx. pipiens hosts.
Víctor Rodríguez‐Valencia, Marie‐Marie Olive, Gilbert Le Goff, Marine Faisse, Marie Bourel, Grégory L'Ambert, Benjamin Vollot, María José Tolsá‐García, Christophe Paupy, David Roiz   +9 more
wiley   +1 more source

Engineering an optimized hypercompact CRISPR/Cas12j‐8 system for efficient genome editing in plants

Plant Biotechnology Journal, Volume 23, Issue 4, Page 1153-1164, April 2025.
Summary The Cas12j‐8 nuclease, derived from the type V CRISPR system, is approximately half the size of Cas9 and recognizes a 5′‐TTN‐3′ protospacer adjacent motif sequence, thus potentially having broad application in genome editing for crop improvement. However, its editing efficiency remains low in plants. In this study, we rationally engineered both
Shasha Bai, Xingyu Cao, Lizhe Hu, Danling Hu, Dongming Li, Yongwei Sun   +5 more
wiley   +1 more source

Analysis of the impact of a uracil DNA glycosylase attenuated in AP-DNA binding in maintenance of the genomic integrity in Escherichia coli [PDF]

, 2010
Uracil DNA glycosylase (Ung) initiates the uracil excision repair pathway. We have earlier characterized the Y66W and Y66H mutants of Ung and shown that they are compromised by ∼7- and ∼170-fold, respectively in their uracil excision activities.
Acharya, Acharya, Aravind, Barrett, Blaisdell, Blattner, Caradonna, Cone, Datsenko, Demple, Dianov, Dianov, Domena, Emsley, Fels, Friedberg, Graves, Guillet, Handa, Handa, Handa, Imlay, Kavli, Krokan, Krusong, Lancaster, Lin, Lindahl, Lindahl, Lindahl, Lindahl, Loeb, Lu, Miller, Otterlei, Parikh, Payne, Pope, Putnam, Ravishankar, Reid, Roy, Sambrook, Sanjay Kumar Bharti, Snowden, Sohail, Spek, Srinath, Talpaert-Borle, Umesh Varshney, Van Der Spoel, Venkatesh, Vieira, Warner, Waters, Williams, Yu, Yu   +57 more
core   +2 more sources

A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping [PDF]

, 2018
Efficient γ-herpesvirus lytic phase replication requires a virally encoded UNG-type uracil-DNA glycosylase as a structural element of the viral replisome.
Bagneris, Claire, Barrett, Tracey, Cole, Ambrose, Earl, Chris, Savva, Renos, Zeman, K.   +5 more
core   +2 more sources

Onset of deaminase APOBEC3B induction in response to DNA double-strand breaks

Biochemistry and Biophysics Reports, 2018
Deamination of 5-methyl cytosine is a major cause of cancer-driver mutations in inflammation-associated cancers. The deaminase APOBEC3B is expressed in these cancers and causes mutations under replication stress; however, the mechanisms by which APOBEC3B
Atsuhiro Shimizu, Haruka Fujimori, Yusuke Minakawa, Yusuke Matsuno, Mai Hyodo, Yasufumi Murakami, Ken-ichi Yoshioka   +6 more
doaj  

Harnessing novel cytidine deaminases from the animal kingdom for robust multiplexed base editing in rice

Plant Biotechnology Journal, Volume 23, Issue 5, Page 1702-1712, May 2025.
Summary CRISPR‐Cas‐based cytosine base editors (CBEs) are prominent tools that perform site‐specific and precise C‐to‐T conversions catalysed by cytidine deaminases. However, their use is often constrained by stringent editing preferences for genomic contexts, off‐target effects and restricted editing windows.
Danyel Fernandes Contiliani, Simon Sretenovic, Micah Dailey, Man Zhou, Yanhao Cheng, Silvana Creste, Shunyuan Xiao, Yiping Qi   +7 more
wiley   +1 more source

Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase. [PDF]

, 1997
3-methyladenine (3MeA) DNA glycosylases remove 3MeAs from alkylated DNA to initiate the base excision repair pathway. Here we report the generation of mice deficient in the 3MeA DNA glycosylase encoded by the Aag (Mpg) gene.
Allan, J.M. (James), Broekhof, J.L.M. (Jose'), Donker, I. (Ingrid), Engelward, B.P. (Bevin), Gold, B. (Bert), Hoeijmakers, J.H.J. (Jan), Samson, L.D. (Leona), Weeda, G. (Geert), Wit, J. (Jan) de, Wyatt, M.D.   +9 more
core   +3 more sources

Base excision by thymine DNA glycosylase mediates DNA-directed cytotoxicity of 5-fluorouracil.

PLoS Biology, 2009
5-Fluorouracil (5-FU), a chemotherapeutic drug commonly used in cancer treatment, imbalances nucleotide pools, thereby favoring misincorporation of uracil and 5-FU into genomic DNA.
Christophe Kunz, Frauke Focke, Yusuke Saito, David Schuermann, Teresa Lettieri, Jim Selfridge, Primo Schär   +6 more
doaj   +1 more source

Roles of uracil-DNA glycosylase and dUTPase in virus replication

Journal of General Virology, 2002
Herpesviruses and poxviruses are known to encode the DNA repair enzyme uracil-DNA glycosylase (UNG), an enzyme involved in the base excision repair pathway that specifically removes the RNA base uracil from DNA, while at least one retrovirus (human immunodeficiency virus type 1) packages cellular UNG into virus particles.
Renxiang Chen, Louis M. Mansky, Huating Wang   +2 more
openaire   +3 more sources

DNA glycosylases: in DNA repair and beyond [PDF]

, 2018
The base excision repair machinery protects DNA in cells from the damaging effects of oxidation, alkylation, and deamination; it is specialized to fix single-base damage in the form of small chemical modifications. Base modifications can be mutagenic and/
Jacobs, Angelika, Schär, Primo
core