Results 221 to 230 of about 22,001 (241)
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Mutation of the uracil DNA glycosylase gene detected in glioblastoma
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1998Despite extensive characterization of genetic changes in gliomas, the underlying etiology of these tumors remains largely unknown. Spontaneous DNA damage due to hydrolysis, methylation, and oxidation is a frequent event in the brain. Failure of DNA repair following this damage may contribute to tumorigenesis of gliomas. Uracil DNA glycosylase (UDG), an
Alexander O. Vortmeyer +7 more
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Inhibition of Uracil DNA Glycosylase by an Oxacarbenium Ion Mimic
Biochemistry, 2002We have investigated the inhibition of the DNA repair enzyme uracil DNA glycosylase (UDG) by an 11-mer oligonucleotide (AIA) containing a cationic 1-aza-deoxyribose (I) residue designed to be a stable mimic of the high-energy oxacarbenium ion reaction intermediate [Werner, R. M., and Stivers, J. T. (2000) Biochemistry 39, 14054-14064].
Yoshitaka Ichikawa +2 more
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Multiple uracil-DNA glycosylase activities in Deinococcus radiodurans
DNA Repair, 2004The extremely radiation resistant bacterium, Deinococcus radiodurans, contains a spectrum of genes that encode for multiple activities that repair DNA damage. We have cloned and expressed the product of three predicted uracil-DNA glycosylases to determine their biochemical function.
Michael J. Daly +4 more
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Recognition of an Unnatural Difluorophenyl Nucleotide by Uracil DNA Glycosylase
Biochemistry, 2004The DNA repair enzyme uracil DNA glycosylase (UDG) utilizes base flipping to recognize and remove unwanted uracil bases from the genome but does not react with its structural congener, thymine, which differs by a single methyl group. Two factors that determine whether an enzyme flips a base from the duplex are its shape and hydrogen bonding properties.
James T. Stivers +8 more
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The cloning and overproduction of Escherichia coli uracil-DNA glycosylase
Gene, 1984Escherichia coli cells containing elevated levels of the DNA repair enzyme uracil-DNA glycosylase (the ung gene product) have been constructed by in vitro recombination methods. First, lambdanadB transducing phages were isolated from two E. coli DNA libraries by selection of nicotinate-independent lysogens.
Judith A. Chambers, Bruce K. Duncan
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Uracil-DNA glycosylase defective mutants of Ustilago maydis
Current Genetics, 1983Uracil-DNA glycosylase activity can be measured in cell-free extracts of Ustilago maydis and in its general properties the enzyme resembles the same glycosylase from other organisms. A rapid assay was used to screen nearly 1,000 clones from cells treated with N-methyl-N'-nitro-N-nitrosoguanidine, and five isolates had
Yoko Yamamoto, Robin Holliday
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Expression and occurrence of uracil-DNA glycosylase in higher plants
Physiologia Plantarum, 1993Uracil‐DNA glycosylase (UDG) is the first enzyme in the base excision repair pathway for removal of uracil in DNA. DNA repair capacity is likely to be a critical factor in mutagenesis and thereby in the capacity to prevent genetic damage and unwanted variation. We have studied expression of UDG in 9 higher plant species.
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Purification and properties of the human placental uracil DNA glycosylase
Biochimica et Biophysica Acta (BBA) - General Subjects, 1987Human placental uracil DNA glycosylase was purified 3700-fold to apparent homogeneity as defined by SDS gel analysis. Its immunological characteristics were examined using three monoclonal antibodies prepared against partially purified human placental uracil DNA glycosylase.
Michael A. Sirover, P. Arenaz, Gita Seal
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Quantitative assays for uracil-DNA glycosylase of high sensitivity
Biochemistry and Cell Biology, 1988We have developed a sensitive fluorometric assay using bisulfite deaminated (C → U), covalently-closed circular PM2 DNA as the substrate. We describe a reliable way to prepare this substrate without nicking the PM2 DNA. Methods, which depend on toluenization of the cells, are described for reproducibly and quantitatively assaying uracil-DNA ...
J Chlebek, A R Morgan
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Uracil-DNA glycosylase activity from Dictyostelium discoideum
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1986We have isolated and partially characterized a uracil-DNA glycosylase activity from the cellular slime mold, Dictyostelium discoideum. This glycosylase has a broad pH optimum (6.5-8.5) and is fully active in 10 mM EDTA or in 5 mM Mg2+. Its molecular weight by gel filtration is about 55 000.
James M. Nonnemaker +2 more
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