Results 231 to 240 of about 22,001 (241)
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Modified substrates as probes for studying uracil-DNA glycosylase
Gene, 1995In order to study the mechanism of action of uracil-DNA glycosylase (UDG) from human placenta, single-stranded (ss) and double-stranded (ds) oligodeoxyribonucleotides (oligos), containing deoxyuridine (dU) and a wide variety of their analogs were used. It was shown that UDG has a twofold preference for ss oligos over ds oligos and a twofold preference ...
Elena A. Kubareva +9 more
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Uracil DNA Glycosylase: Insights from a Master Catalyst
Archives of Biochemistry and Biophysics, 2001The recognition and removal of damaged bases in the genome is the province of a highly specialized assemblage of enzymes known as DNA glycosylases. In recent years, structural and mechanistic studies have rapidly moved forward such that in some cases, the high-resolution structures of all stable complexes along the reaction pathway are available.
Alexander C. Drohat, James T. Stivers
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1999
Uracil in DNA results from either misincorporation of dUMP residues during replication, or from deamination of cytosine residues. The latter process results in premutagenic U:G mispairs that, unless uracil is removed, will cause GC→AT transitions in the subsequent round of replication.
Frank Skorpen +9 more
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Uracil in DNA results from either misincorporation of dUMP residues during replication, or from deamination of cytosine residues. The latter process results in premutagenic U:G mispairs that, unless uracil is removed, will cause GC→AT transitions in the subsequent round of replication.
Frank Skorpen +9 more
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Analysis of uracil DNA glycosylase in human colorectal cancer
International Journal of Oncology, 2001Uracil DNA glycosylase (UDG) is responsible for the removal of uracil present in DNA after cytosine deamination or misincorporation during replication. Colorectal cancer is widely treated with 5-FU, which leads to thymidylate synthase inhibition; this accounts for increased dUTP intracellular pools and subsequent uracil incorporation into DNA.
Ron A. Keenan +5 more
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[36] Uracil-DNA glycosylase from Escherichia coli
1980Publisher Summary This chapter discusses the assay methods and procedures for the isolation of uracil-DNA glycosylase from Escherichia coli . Uracil-DNA glycosylase has been isolated from several different E. coli K12 strains in laboratory. DNA glycosylases catalyze the hydrolysis of base-sugar bonds in DNA. Several different DNA glycosylases have
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Uracil-DNA glycosylase activity in human acute leukemia
Leukemia Research, 1987The expression of the DNA excision repair enzyme uracil-DNA glycosylase was investigated in bone marrow and peripheral samples from seven patients with acute lymphoblastic leukemia (ALL), from 17 patients with acute non-lymphocytic leukemia (ANLL), and from one patient with chronic granulocytic leukemia (CGL) in blast crisis.
Juhani A. Vilpo +2 more
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Uracil-DNA glycosylase activity in chronic lymphoproliferative disorders
Leukemia Research, 1986The activity of uracil-DNA glycosylase, a repair enzyme for the excision of uracil from DNA, was studied in patients with chronic lymphoproliferative disorders and with malignant plasma cell dyscrasias. The biochemical assay was performed on mononuclear cells, isolated by density gradient centrifugation from peripheral blood, from bone marrow or from ...
Erkki Eerola +2 more
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Mechanism of action of uracil-DNA glycosylases
Mutation Research/Environmental Mutagenesis and Related Subjects, 1988J.P. Leblanc +5 more
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