Results 31 to 40 of about 24,501 (233)
Uracil-containing DNA in Drosophila: stability, stage-specific accumulation, and developmental involvement. [PDF]
PLoS Genetics, 2012 Base-excision repair and control of nucleotide pools safe-guard against permanent uracil accumulation in DNA relying on two key enzymes: uracil-DNA glycosylase and dUTPase.
Villő Muha +12 more
doaj +1 more source
Uracil-DNA glycosylases preferentially excise mispaired uracil [PDF]
Biochemical Journal, 1992 We have investigated the substrate specificity of human, viral and bacterial uracil-DNA glycosylases employing as substrate double-stranded oligonucleotides containing in the same position of the 5′-32P-labelled strand an uracil residue facing, on the complementary strand, guanine (mimicking cytosine deamination) or adenine (mimicking dUTP ...
VERRI A +3 more
openaire +3 more sources
Bacillus Calmette-Guerin Increases Base Excision Repair in Bladder Cancer Cells
Journal of Urological Surgery, 2021 Objective:Most patients with non-muscle-invasive bladder cancer (NMIBC) do not respond to intravesical Bacillus Calmette-Guerin (BCG) immunotherapy and have high risk of NMIBC recurrence and progression.
Selçuk Keskin +2 more
doaj +1 more source
FACT Assists Base Excision Repair by Boosting the Remodeling Activity of RSC. [PDF]
PLoS Genetics, 2016 FACT, in addition to its role in transcription, is likely implicated in both transcription-coupled nucleotide excision repair and DNA double strand break repair.
John Lalith Charles Richard +11 more
doaj +1 more source
Uracil-DNA glycosylase of thermophilic Thermothrix thiopara [PDF]
Journal of Bacteriology, 1985 An activity which released free uracil from dUMP-containing DNA was purified approximately 1,700-fold from extracts of Thermothrix thiopara, the first such activity to be isolated from extremely thermophilic bacteria. The enzyme appeared homogeneous, according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
O K, Kaboev +2 more
openaire +2 more sources
Chicken MBD4 Regulates Immunoglobulin Diversification by Somatic Hypermutation
Frontiers in Immunology, 2019 Immunoglobulin (Ig) diversification occurs via somatic hypermutation (SHM) and class switch recombination (CSR), and is initiated by activation-induced deaminase (AID), which converts cytosine to uracil.
Ryan Costello +2 more
doaj +1 more source
Frontiers in Immunology, 2021 Uracil arises in cellular DNA by cytosine (C) deamination and erroneous replicative incorporation of deoxyuridine monophosphate opposite adenine. The former generates C → thymine transition mutations if uracil is not removed by uracil-DNA glycosylase ...
Marina Alexeeva +13 more
doaj +1 more source
A unique uracil-DNA binding protein of the uracil DNA glycosylase superfamily [PDF]
Nucleic Acids Research, 2015 Uracil DNA glycosylases (UDGs) are an important group of DNA repair enzymes, which pioneer the base excision repair pathway by recognizing and excising uracil from DNA. Based on two short conserved sequences (motifs A and B), UDGs have been classified into six families. Here we report a novel UDG, UdgX, from Mycobacterium smegmatis and other organisms.
Sang, Pau Biak +4 more
openaire +3 more sources
Heat‐labile uracil‐DNA glycosylase: purification and characterization [PDF]
FEBS Letters, 1996 A uracil‐DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23 400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat‐labile in dilute buffers (free of stabilizers) with a half‐life of 2 min at 40°C.
Sobek, H. +3 more
openaire +2 more sources
DNA Base Excision Repair Intermediates Influence Duplex–Quadruplex Equilibrium
Molecules, 2023 Although genomic DNA is predominantly duplex under physiological conditions, particular sequence motifs can favor the formation of alternative secondary structures, including the G-quadruplex.
Mark L. Sowers +5 more
doaj +1 more source