Results 31 to 40 of about 22,001 (241)

Intrinsic Strand-Incision Activity of Human UNG: Implications for Nick Generation in Immunoglobulin Gene Diversification

Frontiers in Immunology, 2021
Uracil arises in cellular DNA by cytosine (C) deamination and erroneous replicative incorporation of deoxyuridine monophosphate opposite adenine. The former generates C → thymine transition mutations if uracil is not removed by uracil-DNA glycosylase ...
Marina Alexeeva, Marivi Nabong Moen, Marivi Nabong Moen, Xiang Ming Xu, Anette Rasmussen, Ingar Leiros, Finn Kirpekar, Arne Klungland, Lene Alsøe, Lene Alsøe, Hilde Nilsen, Hilde Nilsen, Svein Bjelland, Svein Bjelland   +13 more
doaj   +1 more source

Tertiary Structure of Uracil-DNA Glycosylase Inhibitor Protein [PDF]

Journal of Biological Chemistry, 1995
The Bacillus subtilis bacteriophage PBS2 uracil-DNA glycosylase inhibitor (Ugi) is an acidic protein of 84 amino acids that inactivates uracil-DNA glycosylase from diverse organisms (Wang, Z., and Mosbaugh, D. W. (1989) J. Biol. Chem. 264, 1163-1171). The secondary structure of Ugi has been determined by solution state multidimensional nuclear magnetic
Suganthi Balasubramanian, Samuel E. Bennett, Philip H. Bolton, Dale W. Mosbaugh, Richard D. Beger   +4 more
openaire   +5 more sources

Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil-DNA glycosylase [PDF]

, 2013
Uracil-DNA glycosylase (UDG) compromises the replication strategies of diverse viruses from unrelated lineages. Virally encoded proteins therefore exist to limit, inhibit or target UDG activity for proteolysis.
A. R. Cole, Acharya, Barrett, Berkner, Broennimann, dos Reis, Duncan, Emsley, Freitag, G. Baltulionis, Haushalter, Hennessy, Huang, Jern, K. Ryzhenkova, Kabsch, Katz, Kiljunen, Kleywegt, Koulis, Martomo, McCoy, Newman, P. Hornyak, Pearl, Perez-Lago, Putnam, Putnam, R. Savva, S. Ofer, Savva, Savva, Savva, Schorman, Sharp, Takahashi, Tye, Warner, Warren, Winn   +39 more
core   +1 more source

A rapid reaction analysis of uracil DNA glycosylase indicates an active mechanism of base flipping [PDF]

, 2007
Uracil DNA glycosylase (UNG) is the primary enzyme for the removal of uracil from the genome of many organisms. A key question is how the enzyme is able to scan large quantities of DNA in search of aberrant uracil residues.
Baldwin, Geoff S., Bellamy, Stuart R.W., Krusong, Kuakarun   +2 more
core   +4 more sources

DNA Base Excision Repair Intermediates Influence Duplex–Quadruplex Equilibrium

Molecules, 2023
Although genomic DNA is predominantly duplex under physiological conditions, particular sequence motifs can favor the formation of alternative secondary structures, including the G-quadruplex.
Mark L. Sowers, James W. Conrad, Bruce Chang-Gu, Ellie Cherryhomes, Linda C. Hackfeld, Lawrence C. Sowers   +5 more
doaj   +1 more source

Uracil-DNA glycosylase in insects

Journal of Biological Chemistry, 1989
It has been reported that Drosophila lacks a uracil-DNA glycosylase but that a direct incising activity on uracil-containing DNA appeared developmentally only in third instar larvae. In contrast we have found by two independent assays, that uracil-DNA glycosylase exists in both Drosophila eggs as well as in third instar larvae.
A R Morgan, J Chlebek
openaire   +3 more sources

Single-strand selective monofunctional uracil-DNA glycosylase (SMUG1) deficiency is linked to aggressive breast cancer and predicts response to adjuvant therapy [PDF]

, 2013
Uracil in DNA is an important cause of mutagenesis. SMUG1 is a uracil DNA glycosylase that removes uracil through base excision repair. SMUG1 also processes radiation induced oxidative base damage as well as 5-fluorouracil incorporated into DNA during
Abdel-Fatah, TMA, Agarwal, D, Albarakati, N, Ball, G, Bowell, L, Chan, S, Ellis, IO, Hawkes, C, Madhusudan, S, Moseley, P   +9 more
core   +1 more source

The interplay of mutations and electronic properties in disease-related genes [PDF]

Scientific Reports 2, 272-9 (2012), 2011
Electronic properties of DNA are believed to play a crucial role in many phenomena in living organisms, for example the location of DNA lesions by base excision repair (BER) glycosylases and the regulation of tumor-suppressor genes such as p53 by detection of oxidative damage.
arxiv   +1 more source

Correlated Mutation in the Evolution of Catalysis in Uracil DNA Glycosylase Superfamily. [PDF]

Sci Rep, 2017
Enzymes in Uracil DNA glycosylase (UDG) superfamily are essential for the removal of uracil. Family 4 UDGa is a robust uracil DNA glycosylase that only acts on double-stranded and single-stranded uracil-containing DNA.
Xia B, Liu Y, Guevara J, Li J, Jilich C, Yang Y, Wang L, Dominy BN, Cao W.   +8 more
europepmc   +3 more sources

Uracil-DNA glycosylases preferentially excise mispaired uracil [PDF]

Biochemical Journal, 1992
We have investigated the substrate specificity of human, viral and bacterial uracil-DNA glycosylases employing as substrate double-stranded oligonucleotides containing in the same position of the 5′-32P-labelled strand an uracil residue facing, on the complementary strand, guanine (mimicking cytosine deamination) or adenine (mimicking dUTP ...
VERRI A, MAZZARELLO P, SPADARI S, FOCHER F   +3 more
openaire   +4 more sources