Results 41 to 50 of about 22,001 (241)
Structure and stereochemistry of the base excision repair glycosylase MutY reveal a mechanism similar to retaining glycosidases. [PDF]
, 2015 MutY adenine glycosylases prevent DNA mutations by excising adenine from promutagenic 8-oxo-7,8-dihydroguanine (OG):A mismatches. Here, we describe structural features of the MutY active site bound to an azaribose transition state analog which indicate a
Cao, Sheng +6 more
core +2 more sources
Scientific Reports, 2017 Both a DNA lesion and an intermediate for antibody maturation, uracil is primarily processed by base excision repair (BER), either initiated by uracil-DNA glycosylase (UNG) or by single-strand selective monofunctional uracil DNA glycosylase (SMUG1).
Lene Alsøe +15 more
doaj +1 more source
Heat‐labile uracil‐DNA glycosylase: purification and characterization [PDF]
FEBS Letters, 1996 A uracil‐DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23 400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat‐labile in dilute buffers (free of stabilizers) with a half‐life of 2 min at 40°C.
Harald Sobek +3 more
openaire +3 more sources
A Method for Constructing Nucleosome Arrays with Spatially Defined Histone PTMs and DNA Damage
Angewandte Chemie, EarlyView.This work advances methodology by addressing challenges in mimicking the complex in vivo spatial relationships of histone acetylation and DNA damage. The combination of an abiotic/enzymatic hybrid catalyst system (ABEHCS) and the plug‐and‐play method has constituted a versatile platform to construct nucleosome arrays with precise histone acetylation ...
Ziyun Liu +6 more
wiley +2 more sources
BioTechniques, 2022 This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase.
Naoki Harikai +4 more
doaj +1 more source
Direct Measurement of the Substrate Preference of Uracil-DNA Glycosylase [PDF]
Journal of Biological Chemistry, 1998 Site-directed mutants of the herpes simplex virus type 1 uracil-DNA glycosylase lacking catalytic activity have been used to probe the substrate recognition of this highly conserved and ubiquitous class of DNA-repair enzyme utilizing surface plasmon resonance. The residues aspartic acid-88 and histidine-210, implicated in the catalytic mechanism of the
Panayotou, G +4 more
openaire +3 more sources
A Novel 3-Methyladenine DNA Glycosylase from Helicobacter pylori Defines a New Class within the Endonuclease III Family of Base Excision Repair Glycosylases [PDF]
, 2000 The cloning, purification, and characterization of MagIII, a 3-methyladenine DNA glycosylase from Helicobacter pylori, is presented in this paper. Sequence analysis of the genome of this pathogen failed to identify open reading frames potentially coding ...
Boiteux, Serge +5 more
core +1 more source
Plant mitochondria possess a short-patch base excision DNA repair pathway [PDF]
, 2009 Despite constant threat of oxidative damage, sequence drift in mitochondrial and chloroplast DNA usually remains very low in plant species, indicating efficient defense and repair.
Boesch, Pierre +5 more
core +2 more sources
Primer extension mutagenesis powered by selective rolling circle amplification. [PDF]
PLoS ONE, 2012 Primer extension mutagenesis is a popular tool to create libraries for in vitro evolution experiments. Here we describe a further improvement of the method described by T.A.
Tuomas Huovinen +6 more
doaj +1 more source