Results 51 to 60 of about 24,501 (233)

Suppression of Uracil-DNA Glycosylase Induces Neuronal Apoptosis [PDF]

Journal of Biological Chemistry, 2004
A chronic imbalance in DNA precursors, caused by one-carbon metabolism impairment, can result in a deficiency of DNA repair and increased DNA damage. Although indirect evidence suggests that DNA damage plays a role in neuronal apoptosis and in the pathogenesis of neurodegenerative disorders, the underlying mechanisms are poorly understood.
Inna I, Kruman, Elena, Schwartz, Yuri, Kruman, Roy G, Cutler, Xiaoxiang, Zhu, Nigel H, Greig, Mark P, Mattson   +6 more
openaire   +2 more sources

Primer extension mutagenesis powered by selective rolling circle amplification. [PDF]

PLoS ONE, 2012
Primer extension mutagenesis is a popular tool to create libraries for in vitro evolution experiments. Here we describe a further improvement of the method described by T.A.
Tuomas Huovinen, Eeva-Christine Brockmann, Sultana Akter, Susan Perez-Gamarra, Jani Ylä-Pelto, Yuan Liu, Urpo Lamminmäki   +6 more
doaj   +1 more source

Uracil-DNA Glycosylase Inhibitor Gene of Bacteriophage PBS2 Encodes a Binding Protein Specific for Uracil-DNA Glycosylase

Journal of Biological Chemistry, 1989
The uracil-DNA glycosylase inhibitor gene (ugi) of the Bacillus subtilis bacteriophage PBS2 has been subcloned to a 720-base pair DNA fragment contained in pZW2-0.7 and its nucleotide sequence determined. Using nucleotide deletion analysis, we have located the cloned ugi gene along with potential regulatory elements. A promoter-like region (-10 and -35
Z, Wang, D W, Mosbaugh
openaire   +2 more sources

Action mechanism of human SMUG1 uracil-DNA glycosylase [PDF]

Nucleic Acids Symposium Series, 2005
The DNA lesions resulting from deamination or oxidation of bases are generally repaired by the base excision repair pathway initiated by damage-specific DNA glycosylases. Single-strand selective monofunctional uracil-DNA glycosylase (SMUG1) present in vertebrates and insects excises not only uracil but also uracil derivatives bearing an oxidized group ...
Mayumi, Matsubara, Tamon, Tanaka, Hiroaki, Terato, Hiroshi, Ide   +3 more
openaire   +2 more sources

Biochemical characterization and novel inhibitor identification of Mycobacterium tuberculosis Endonuclease VIII 2 (Rv3297)

Biochemistry and Biophysics Reports, 2017
Nei2 (Rv3297) is a DNA Base Excision Repair (BER) glycosylase that is essential for survival of Mycobacterium tuberculosis in primates. We show that MtbNei2 is a bifunctional glycosylase that specifically acts on oxidized pyrimidine-containing single ...
Kiran Lata, Mohammad Afsar, Ravishankar Ramachandran   +2 more
doaj   +1 more source

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

Advanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son, Goeun Kim, Jungjoon K. Lee   +2 more
wiley   +1 more source

RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control

Biomolecules, 2021
RNA modifications are essential for proper RNA processing, quality control, and maturation steps. In the last decade, some eukaryotic DNA repair enzymes have been shown to have an ability to recognize and process modified RNA substrates and thereby ...
Lisa Lirussi, Özlem Demir, Panpan You, Antonio Sarno, Rommie E. Amaro, Hilde Nilsen   +5 more
doaj   +1 more source

Mutations at Arginine 276 transform human uracil-DNA glycosylase into a single-stranded DNA-specific uracil-DNA glycosylase [PDF]

DNA Repair, 2005
To investigate the role of Arginine 276 in the conserved leucine-loop of human uracil-DNA glycosylase (UNG), the effects of six R276 amino acid substitutions (C, E, H, L, W, and Y) on nucleotide flipping and enzyme conformational change were determined using transient and steady state, fluorescence-based, kinetic analysis.
Cheng-Yao, Chen, Dale W, Mosbaugh, Samuel E, Bennett   +2 more
openaire   +2 more sources

Calcium Channel Blockers Inhibit Pancreatic Neuroendocrine Neoplasms Progression via Cav1.2‐Epigenetic Circuit

Advanced Science, EarlyView.
Our study reveals a novel mechanism of a positive regulatory circuit between Cav1.2 and H3K27ac for pancreatic neuroendocrine neoplasms (pNENs) progression. Cav1.2 is identified as a crucial target for promoting disease progression and correlates with malignant behaviors, which are remarkably inhibited by the administration of calcium channel blockers (
Yangyinhui Yu, Qiongcong Xu, Jinzhao Xie, Mingjian Ma, Xitai Huang, Yinhao Shi, Jiawei Zhou, Enliang Zhu, Ziyi Zhao, Ning Zhang, Zhide Liu, Jingyuan Ye, Xiaoyu Yin   +12 more
wiley   +1 more source

Uracil DNA glycosylase counteracts APOBEC3G-induced hypermutation of hepatitis B viral genomes: excision repair of covalently closed circular DNA. [PDF]

PLoS Pathogens, 2013
The covalently closed circular DNA (cccDNA) of the hepatitis B virus (HBV) plays an essential role in chronic hepatitis. The cellular repair system is proposed to convert cytoplasmic nucleocapsid (NC) DNA (partially double-stranded DNA) into cccDNA in ...
Kouichi Kitamura, Zhe Wang, Sajeda Chowdhury, Miyuki Simadu, Miki Koura, Masamichi Muramatsu   +5 more
doaj   +1 more source