Results 61 to 70 of about 24,501 (233)

Epigenotoxicity: Decoding the epigenetic imprints of genotoxic agents and their implications for regulatory genetic toxicology

Environmental and Molecular Mutagenesis, EarlyView.
Abstract Regulatory genetic toxicology focuses on DNA damage and subsequent gene mutations. However, genotoxic agents can also affect epigenetic marks, and incorporation of epigenetic data into the regulatory framework may thus enhance the accuracy of risk assessment.
Roger Godschalk, Christopher Faulk, Jessica LaRocca, Jan van Benthem, Francesco Marchetti   +4 more
wiley   +1 more source

A common SNP in the UNG gene decreases ovarian cancer risk in BRCA2 mutation carriers

Molecular Oncology, 2019
Single nucleotide polymorphisms (SNPs) in DNA glycosylase genes involved in the base excision repair (BER) pathway can modify breast and ovarian cancer risk in BRCA1 and BRCA2 mutation carriers.
Juan Miguel Baquero, Carlos Benítez‐Buelga, Victoria Fernández, Miguel Urioste, Jose Luis García‐Giménez, Rosario Perona, the CIMBA Consortium, Javier Benítez, Ana Osorio   +8 more
doaj   +1 more source

Unscheduled DNA synthesis leads to elevated uracil residues at highly transcribed genomic loci in Saccharomyces cerevisiae.

PLoS Genetics, 2018
Recombination and mutagenesis are elevated by active transcription. The correlation between transcription and genome instability is largely explained by the topological and structural changes in DNA and the associated physical obstacles generated by the ...
Norah Owiti, Shanqiao Wei, Ashok S Bhagwat, Nayun Kim   +3 more
doaj   +1 more source

Noncanonical Nucleotides in the Genome Around the Maternal‐Zygotic Transition

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
In this paper, Kazzazi et al. provide a comprehensive review of the dynamics of nonconventional nucleotides in the genome during early developmental stages, hypothesizing a potential role for these nucleotides in the activation of the zygotic genome. ABSTRACT From the very moment of fertilization and throughout development, the cells of animal embryos ...
Latifa Kazzazy, Dávid Mező, Kinga K. Nagy, Viktória Perey‐Simon, Judit Tóth, Angéla Békési, Beáta Vértessy, Máté Varga   +7 more
wiley   +1 more source

Predators of the two paropsine leaf beetles Paropsisterna cloelia and Paropsis charybdis in eucalypt plantations in Marlborough, New Zealand Prädatoren der zwei Blattkäfer Paropsisterna cloelia and Paropsis charybdis in Eukalyptusplantagen in Marlborough, Neuseeland

Agricultural and Forest Entomology, EarlyView.
Coccinellidae (Coleoptera), Miridae (Hemiptera), Hemerobiidae (Neuroptera), Pentatomidae (Hemiptera), Anystidae (Acari), Erythraeidae (Acari) and spiders (Araneidae, Oxyopidae and Salticidae) fed on the invasive paropsine leaf beetles in Marlborough, New Zealand.
Carolin Weser, Manpreet K. Dhami, Elena Moltchanova, Ana Podolyan, Toni M. Withers, Stephen M. Pawson   +5 more
wiley   +1 more source

[Uracil-DNA glycosylases].

Postepy biochemii, 2009
Uracil is one of four nitrogen bases, most frequently found in normal RNA. Uracyl can be found also in DNA as a result of enzymatic or non-enzymatic deamination of cytosine as well as misincorporation of dUMP instead of dTMP during DNA replication. Uracil from DNA can be removed by DNA repair enzymes with apirymidine site as an intermediate.
Dariusz, Pytel, Artur, Słupianek, Dominika, Ksiazek, Tomasz, Skórski, Janusz, Błasiak   +4 more
openaire   +1 more source

Genome‐wide and gene‐specific DNA methylation across developmental stages in Pogonomyrmex californicus: A socially polymorphic ant

Insect Molecular Biology, EarlyView.
Comparison between developmental stages (larvae, pupae, worker) in Pogonomyrmex californicus revealed significant stage‐specific differences in Gene Body Methylated frequencies. Methylation sites were highly correlated between WGBS and ONT in P. californicus Genome‐wide methylation was low (~3%) and highly clustered within gene bodies (GBM), especially
Tania Chavarria‐Pizarro, Mohammed Errbii, Janina Rinke, Lukas Schrader, Jürgen Gadau   +4 more
wiley   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

The ‘very moment’ when UDG recognizes a flipped-out uracil base in dsDNA

Scientific Reports
Uracil-DNA glycosylase (UDG) is the first enzyme in the base-excision repair (BER) pathway, acting on uracil bases in DNA. How UDG finds its targets has not been conclusively resolved yet.
Vinnarasi Saravanan, Nessim Raouraoua, Guillaume Brysbaert, Stefano Giordano, Marc F. Lensink, Fabrizio Cleri, Ralf Blossey   +6 more
doaj   +1 more source

Cloning and Assembly of PCR Products Using Modified Primers and DNA Repair Enzymes

BioTechniques, 1997
We present a method for the creation of ligatable 3′overhangs by the incorporation of a modified base, uracil, at a specific position in the PCR primer and subsequent treatment with the DNA-modifying enzyme uracil DNA glycosylase and then either T4 ...
David E. Watson, George N. Bennett
doaj   +1 more source