A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation [PDF]
, 2016 Glycosylation greatly influences the safety and efficacy of many of the highest-selling recombinant therapeutic proteins (rTPs). In order to define optimal cell culture feeding strategies that control rTP glycosylation, it is necessary to know how ...
Jimenez del Val, I +2 more
core +1 more source
Establishment of a five-enzyme cell-free cascade for the synthesis of uridine diphosphate N-acetylglucosamine [PDF]
Journal of Biotechnology, 2018 In spite of huge endeavors in cell line engineering to produce glycoproteins with desired and uniform glycoforms, it is still not possible in vivo. Alternatively, in vitro glycoengineering can be used for the modification of glycans. However, in vitro glycoengineering relies on expensive nucleotide sugars, such as uridine 5'-diphospho-N ...
Reza Mahour +7 more
openaire +3 more sources
Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803 [PDF]
, 2020 Cyanobacteria are key organisms in the global ecosystem, useful models for studying metabolic and physiological processes conserved in photosynthetic organisms, and potential renewable platforms for production of chemicals.
Lea-Smith, David +2 more
core +3 more sources
Fueling the fire: emerging role of the hexosamine biosynthetic pathway in cancer
BMC Biology, 2019 Altered metabolism and deregulated cellular energetics are now considered a hallmark of all cancers. Glucose, glutamine, fatty acids, and amino acids are the primary drivers of tumor growth and act as substrates for the hexosamine biosynthetic pathway ...
Neha M. Akella +2 more
doaj +1 more source
Identification of a carbohydrate recognition motif of purinergic receptors
eLife, 2023 As a major class of biomolecules, carbohydrates play indispensable roles in various biological processes. However, it remains largely unknown how carbohydrates directly modulate important drug targets, such as G-protein coupled receptors (GPCRs).
Lifen Zhao +7 more
doaj +1 more source
The role of Alg13 N-acetylglucosaminyl transferase in the expression of pathogenic features of Candida albicans. [PDF]
, 2017 Background: The pathogenic potential of Candida albicans depends on adhesion to the host cells mediated by highly glycosylated adhesins, hyphae formation and growth of biofilm. These factors require effective N-glycosylation of proteins.
Janik, Anna +5 more
core +1 more source
Scientific Reports, 2017 In Bacillus subtilis, Listeria monocytogenes and in two Mycobacteria, it was previously shown that yvcK is a gene required for normal cell shape, for optimal carbon source utilization and for virulence of pathogenic bacteria.
Elodie Foulquier, Anne Galinier
doaj +1 more source
Molecules, 2020 The synthesis of hitherto unknown 5′-deoxy-5′-(4-substituted-1,2,3-triazol-1-yl)-uridine and its evaluation, through an one-pot screening assay, against MurA-F enzymes involved in Mycobacterium tuberculosis (Mtb), are described.
Vincent Hervin +6 more
doaj +1 more source
Cross-Dysregulation of O-GlcNAcylation and PI3K/AKT/mTOR Axis in Human Chronic Diseases
Frontiers in Endocrinology, 2018 The hexosamine biosynthetic pathway (HBP) and the phosphatidylinositol 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signaling pathway are considered as nutrient sensors that regulate several essential biological processes.
Ninon Very +4 more
doaj +1 more source
Disruption of the MreB Elongasome Is Overcome by Mutations in the Tricarboxylic Acid Cycle
Frontiers in Microbiology, 2021 The bacterial actin homolog, MreB, is highly conserved among rod-shaped bacteria and essential for growth under normal growth conditions. MreB directs the localization of cell wall synthesis and loss of MreB results in round cells and death.
Brody Barton +2 more
doaj +1 more source