Results 11 to 20 of about 7,719 (211)

Biosynthesis of uridine diphosphate N-Acetylglucosamine: An underexploited pathway in the search for novel antibiotics? [PDF]

IUBMB Life, 2022
AbstractAlthough the prevalence of antibiotic resistance is increasing at an alarming rate, there are a dwindling number of effective antibiotics available. Thus, the development of novel antibacterial agents should be of utmost importance. Peptidoglycan biosynthesis has been and is still an attractive source for antibiotic targets; however, there are ...
Wyllie JA, McKay MV, Barrow AS, Soares da Costa TP.   +3 more
europepmc   +4 more sources

Uridine diphosphate N-acetylglucosamine orchestrates the interaction of GlmR with either YvcJ or GlmS in Bacillus subtilis. [PDF]

Sci Rep, 2020
AbstractIn bacteria, glucosamine-6-phosphate (GlcN6P) synthase, GlmS, is an enzyme required for the synthesis of Uridine diphosphate N-acetylglucosamine (UDP-GlcNAc), a precursor of peptidoglycan. In Bacillus subtilis, an UDP-GlcNAc binding protein, GlmR (formerly YvcK), essential for growth on non-glycolytic carbon sources, has been proposed to ...
Foulquier E, Pompeo F, Byrne D, Fierobe HP, Galinier A.   +4 more
europepmc   +5 more sources

A novel allosteric inhibitor of the uridine diphosphate N-acetylglucosamine pyrophosphorylase from Trypanosoma brucei. [PDF]

ACS Chem Biol, 2013
Uridine diphosphate N-acetylglucosamine pyrophosphorylase (UAP) catalyzes the final reaction in the biosynthesis of UDP-GlcNAc, an essential metabolite in many organisms including Trypanosoma brucei, the etiological agent of Human African Trypanosomiasis. High-throughput screening of recombinant T. brucei UAP identified a UTP-competitive inhibitor with
Urbaniak MD, Collie IT, Fang W, Aristotelous T, Eskilsson S, Raimi OG, Harrison J, Navratilova IH, Frearson JA, van Aalten DM, Ferguson MA.   +10 more
europepmc   +5 more sources

Structure of uridine diphosphate N-acetylglucosamine pyrophosphorylase from Entamoeba histolytica. [PDF]

Acta Crystallogr F Struct Biol Commun, 2015
Uridine diphosphateN-acetylglucosamine pyrophosphorylase (UAP) catalyzes the final step in the synthesis of UDP-GlcNAc, which is involved in cell-wall biogenesis in plants and fungi and in protein glycosylation. Small-molecule inhibitors have been developed against UAP fromTrypanosoma bruceithat target an allosteric pocket to provide selectivity over ...
Edwards TE, Gardberg AS, Phan IQ, Zhang Y, Staker BL, Myler PJ, Lorimer DD.   +6 more
europepmc   +4 more sources

Multienzyme Platform for the Synthesis of UDP Sugars and Human Milk Oligosaccharides. [PDF]

Chembiochem
Engineered Escherichia coli produces six enzymes (UMPK, PPK3, GALK, NAHK, GALU, PPA) that are purified and used in a cell‐free cascade to synthesize UDP‐N‐acetylglucosamine (UDP‐GlcNAc) and UDP‐galactose (UDP‐Gal) from inexpensive substrates. The cascades can be coupled with glycosyltransferases for in situ nucleotide sugar regeneration, enabling ...
Hoang TS, Lange F, Kleeberg SB, Thomas L, Hoang NH, Reichl U, Rexer TFT.   +6 more
europepmc   +2 more sources

A Mutant Yeast Deficient in Golgi Transport of Uridine Diphosphate N-Acetylglucosamine [PDF]

Journal of Biological Chemistry, 1996
Mannan chains of Kluyveromyces lactis mannoproteins are similar to those of Saccharomyces cerevisiae except that they have terminal alpha1-->2-linked N-acetylglucosamine and lack mannose phosphate. In a previous study, Douglas and Ballou (Douglas, R. K., and Ballou, C. E.
Abeijon, Claudia, Mandon, Elisabet C., Robbins, Phillips W., Hirschberg, Carlos B.   +3 more
openaire   +3 more sources

Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803 [PDF]

, 2020
Cyanobacteria are key organisms in the global ecosystem, useful models for studying metabolic and physiological processes conserved in photosynthetic organisms, and potential renewable platforms for production of chemicals.
Lea-Smith, David, McCormick, Alistair J., Mills, Lauren   +2 more
core   +3 more sources

Isolation of Endogenous Activator of Glucuronide For­mation in Liver [PDF]

, 1962
A purified compound of the activator of glucuronide formation was isolated from a boiled extract of rat liver by charcoal adsorption, ethanol fractionation of barium salts, and finally paper and Dowex-l column chromatographies.
Taketa, Kazuhisa
core   +1 more source

Agarose Derivatives of Uridine Diphosphate and N-Acetylglucosamine for the Purification of a Galactosyltransferase

Journal of Biological Chemistry, 1972
Abstract The applicability of affinity chromatography for the purification of UDP-galactose: N-acetylglucosamine galactosyltransferase has been evaluated. Three different types of specific adsorbents have been synthesized by reaction of a ligand structurally related to portions of the substrates of the transferase with cyanogen bromide-treated agarose.
R, Barker, K W, Olsen, J H, Shaper, R L, Hill   +3 more
openaire   +2 more sources

Uridine diphosphate <i>N</i>-acetylglucosamine homeostasis via CgNgk1 kinase modulates chitin levels and cell-wall integrity in <i>Candida glabrata</i>. [PDF]

FEMS Microbes
Abstract Candida glabrata is a clinically significant cause of candidemia, yet how it supplies uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) for cell-wall chitin synthesis remains unclear. Here, we identify an N-acetylglucosamine (GlcNAc) kinase encoded by CAGL0M00682g and designated CgNgk1.
Yamada R, Nakayama H, Yoshikawa S, Karita S, Umekawa M.   +4 more
europepmc   +3 more sources