Results 1 to 10 of about 17,352,598 (197)

Structural basis for kinase inhibition in the tripartite E. coli HipBST toxin–antitoxin system [PDF]

eLife, 2023
Many bacteria encode multiple toxin–antitoxin (TA) systems targeting separate, but closely related, cellular functions. The toxin of the Escherichia coli hipBA system, HipA, is a kinase that inhibits translation via phosphorylation of glutamyl-tRNA ...
René L Bærentsen, Stine V Nielsen, Ragnhild B Skjerning, Jeppe Lyngsø, Francesco Bisiak, Jan Skov Pedersen, Kenn Gerdes, Michael A Sørensen, Ditlev E Brodersen   +8 more
doaj   +3 more sources

A novel type I toxin-antitoxin system modulates persister cell formation in Staphylococcus aureus

International Journal of Medical Microbiology, 2020
A plethora of toxin-antitoxin systems exist in bacteria and has multilateral roles in bacterial pathogenesis and virulence. Toxin-antitoxin systems have been involved in persister cell formation in Escherichia coli and Mycobacterium but have not been ...
Gul Habib, Jiade Zhu, Baolin Sun
doaj   +4 more sources

Phage nuclease-mediated defense activation of the bacterial Retron-Eco7 toxin–antitoxin system [PDF]

Nucleic Acids Res
Retrons are bacterial antiphage defense systems comprising a reverse transcriptase (RT), a non-coding RNA (ncRNA), and cognate effector proteins. The RT synthesizes multicopy single-stranded DNA (msDNA) from the ncRNA template to detect phage invasion ...
Zhikang Dai, Chang Liu, Yanjing Wang, Xueting Chen, Xi Fu, Kaiyue Yang, Rui-Liang Zhu, Xianyue Jia, Yanke Chen, Pan Tao, Ze-Yuan Guan, Ting-Ting Zou   +11 more
semanticscholar   +2 more sources

A bacterial toxin–antitoxin system as a native defence element against RNA phages [PDF]

Biol Lett
Bacteria have evolved a wide range of defence strategies to protect themselves against bacterial viruses (phages). Most known bacterial antiphage defence systems target phages with DNA genomes, which raises the question of how bacteria defend against ...
Nela Nikolic, Maroš Pleška, T. Bergmiller, C. Guet   +3 more
semanticscholar   +2 more sources

Continuous Evolution System Based on Toxin-Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins. [PDF]

Adv Sci (Weinh)
The promoted Escherichia coli‐assisted continuous evolution (PEACE) system operates through a stringent three step genetic circuit: target gene mutation via a deaminase–T7 RNA polymerase (T7 RNAP) fusion, coupling variant function to antitoxin expression, and growth based selection of surviving cells.
Zhang X, Liu Z, Xu Y, Sun M, Zhou G, Rao D, Chen X, Wang S, Li Z, Huang Y, Wu J.   +10 more
europepmc   +2 more sources

Shutoff of host transcription triggers a toxin-antitoxin system to cleave phage RNA and abort infection

Molecular Cell, 2021
Summary Toxin-antitoxin (TA) systems are widespread in bacteria, but their activation mechanisms and bona-fide targets remain largely unknown. Here, we characterize a type III TA system, toxIN, that protects E.
Michael T Laub
exaly   +2 more sources

A quinolone N-oxide antibiotic selectively targets Neisseria gonorrhoeae via its toxin–antitoxin system [PDF]

Nat Microbiol
Gonorrhoea is a major sexually transmitted infection and the emergence of multidrug-resistant Neisseria gonorrhoeae poses a global health threat. To identify candidate antibiotics against N.
Ann-Kathrin Mix, Thi-Hong Nhung Nguyen, Tamara Schuhmacher, Dávid Szamosvári, Petra Muenzner, P. Haas, Lydia Heeb, H. Wami, U. Dobrindt, Yasar Özge Delikkafa, Thomas U. Mayer, T. Böttcher, Christof R. Hauck   +12 more
semanticscholar   +2 more sources

Effect of ZnO nanoparticles on biofilm formation and gene expression of the toxin-antitoxin system in clinical isolates of Pseudomonas aeruginosa [PDF]

Ann Clin Microbiol Antimicrob, 2023
Background Biofilm formation by Pseudomonas aeruginosa ( P. aeruginosa ) is known to be characteristic of this organism. This bacterium is considered one of the most life-threatening bacteria and has been identified as a priority pathogen for research by
H. Valadbeigi, N. Sadeghifard, Vahab Hassan Kaviar, M. H. Haddadi, S. Ghafourian, Abbas Maleki   +5 more
semanticscholar   +2 more sources

A toxin-antitoxin system provides phage defense via DNA damage and repair [PDF]

Nat Commun
Widespread in bacteria and archaea, toxin-antitoxin (TA) systems have been recently demonstrated to function in phage defense. Here we characterize the anti-phage function of a type IV TA system, ShosTA.
Huan Pu, Yu-Xi Chen, Xin-Jun Zhao, Lunzhi Dai, Aiping Tong, Dong-Mei Tang, Qiang Chen, Yamei Yu   +7 more
semanticscholar   +2 more sources

Potential role of a CRISPR-Cas-activated toxin-antitoxin system in bacterial immunity [PDF]

Nature Communications
CRISPR-Cas and toxin-antitoxin systems can serve as antiviral defense mechanisms in prokaryotes. In typical toxin-antitoxin systems, toxin activation can limit phage propagation by inducing growth arrest or reduced cellular fitness, while the antitoxin ...
Jiyun Chen, Linglong Huang, Hong Chen, Xueyan Li, Xiaofeng Lin, Chenmin Guo, Xi Liu, Guowei Fu, Ying Chen, Liang Liu   +9 more
doaj   +2 more sources