A cell-based assay for CD63-containing extracellular vesicles [PDF]
, 2019 Extracellular vesicles (EVs) are thought to be important in cell-cell communication and have elicited extraordinary interest as potential biomarkers of disease. However, quantitative methods to enable elucidation of mechanisms underlying release are few.
Cashikar, Anil G, Hanson, Phyllis I
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Retrieval of the vacuolar H-ATPase from phagosomes revealed by live cell imaging. [PDF]
PLoS ONE, 2010 BackgroundThe vacuolar H+-ATPase, or V-ATPase, is a highly-conserved multi-subunit enzyme that transports protons across membranes at the expense of ATP.
Margaret Clarke +3 more
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Rapid purification of human lysosomal membranes, characterisation of the detergent resistent microdomains, purification and reconstitution of the vacuolar proton pump (V-ATPase) [PDF]
, 2006 The lysosomal membrane is a dynamic environment where specific interactions among proteins and between proteins and lipids occur. These interactions are necessary for the proper functioning of the lysosomal apparatus that allows the passage of molecules ...
Chandramohanadas, Rajesh +1 more
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V-ATPase engagement in autophagic processes [PDF]
Autophagy, 2011 The proton pumping activity of V-ATPase is responsible for acidification of the lysosome/vacuole. The low lumenal pH of this organelle stimulates the activity of a battery of resident hydrolases responsible for the degradation of various nonselective and selective cargos delivered by autophagic processes.
Rodney J. Devenish +2 more
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F-actin reorganization by V-ATPase inhibition in prostate cancer
Biology Open, 2017 The vacuolar ATPase (V-ATPase) proton pump sustains cellular pH homeostasis, and its inhibition triggers numerous stress responses. However, the cellular mechanisms involved remain largely elusive in cancer cells.
Yamhilette Licon-Munoz +3 more
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Molecular Metabolism, 2021 Objective: The diabetic heart is characterized by extensive lipid accumulation which often leads to cardiac contractile dysfunction. The underlying mechanism involves a pivotal role for vacuolar-type H+-ATPase (v-ATPase, functioning as endosomal ...
Shujin Wang +11 more
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Saccharomyces cerevisiae mutants affected in vacuole assembly or vacuolar H+-ATPase are hypersensitive to lead (Pb) toxicity [PDF]
, 2014 Lead is an important environmental pollutant. The role of vacuole, in Pb detoxification, was studied using a vacuolar protein sorting mutant strain (vps16Δ), belonging to class C mutants.
A Richards +34 more
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The curious case of vacuolar ATPase: regulation of signaling pathways
Molecular Cancer, 2018 The Vacuolar ATPase (V-ATPase) is a proton pump responsible for controlling the intracellular and extracellular pH of cells. The structure of V-ATPase has been highly conserved among all eukaryotic cells and is involved in diverse functions across ...
Sahithi Pamarthy +3 more
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The E3 ubiquitin ligase ZNRF2 is a substrate of mTORC1 and regulates its activation by amino acids [PDF]
, 2016 The mechanistic Target of Rapamycin complex 1 (mTORC1) senses intracellular amino acid levels through an intricate machinery, which includes the Rag GTPases, Ragulator and vacuolar ATPase (V-ATPase).
Appelqvist +41 more
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mTORC1 Senses Lysosomal Amino Acids Through an Inside-Out Mechanism That Requires the Vacuolar H+-ATPase [PDF]
, 2011 The mTOR complex 1 (mTORC1) protein kinase is a master growth regulator that is stimulated by amino acids. Amino acids activate the Rag guanosine triphosphatases (GTPases), which promote the translocation of mTORC1 to the lysosomal surface, the site of ...
Bar-Peled, Liron +5 more
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