FEBS Open Bio, EarlyView.This study optimizes a two‐step induction system to generate PGCLCs from chicken ESCs. The induced cells express germline markers and migrate to gonads in ovo, offering a novel approach for avian genetic resource conservation. Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs.
Zeyu Li +10 more
wiley +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Modeling the Influence of Environment and Intervention on Cholera in Haiti [PDF]
, 2014 We propose a simple model with two infective classes in order to model the cholera epidemic in Haiti. We include the impact of environmental events (rainfall, temperature and tidal range) on the epidemic in the Artibonite and Ouest regions by introducing
Freitag, Caroline +2 more
core +4 more sources
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Promoting Adherence to Influenza Vaccination Recommendations in Pediatric Practice. [PDF]
, 2019 OBJECTIVES: In the United States, nonadherence to seasonal influenza vaccination guidelines for children and adolescents is common and results in unnecessary morbidity and mortality. We conducted a quality improvement project to improve vaccination rates
Cadilla, Adriana +4 more
core +1 more source
The association between life events, social support, and antibody status following thymus-dependent and thymus-independent vaccinations in healthy young adults [PDF]
, 2005 This study determined whether stressful life events and social support were related to antibody status following both thymus-dependent and thymus-independent vaccinations. Life events in the previous year and customary social support were measured in 57
Burns, Victoria E. +4 more
core +1 more source
Hemophagocytic lymphohistiocytosis after COVID-19 vaccination [PDF]
, 2021 Liang Tang, Yu Hu
openalex +1 more source
Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node
FEBS Open Bio, EarlyView.The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner +6 more
wiley +1 more source
Building trust and equity in vaccine communication through community engagement
Human Vaccines & ImmunotherapeuticsThat the COVID-19 pandemic has exacerbated inequities in health has been well studied in recent years, yet the ways in which the pandemic has also revealed existing inequities in communication, specifically health communication, is less well understood ...
Theresa Sommers +10 more
doaj +1 more source
Comparative analysis of viral RNA signatures on different RIG-I-like receptors
eLife, 2016 The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown.
Raul Y Sanchez David +10 more
doaj +1 more source