Results 191 to 200 of about 12,353,923 (294)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Factors influencing sodium restriction behavior among patients with chronic heart failure: a cross-sectional study based on the value co-creation theory. [PDF]

BMC Cardiovasc Disord
Chen S, Qiu X, Dai Y, Deng Z, Liu N, Xie R, Qiu X, Zhang L.   +7 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Study on the influence of intelligent human-computer interaction of AI virtual anchors on consumers' initial trust and value co-creation behavior under the technophobia. [PDF]

Front Psychol
Zhong L, Wang Y, Yue Z, Yang Y.
europepmc   +1 more source

Value Co-creation in the Cloud

, 2017
Enterprise systemen worden meer en meer gehost in de cloud en aangeboden als een dienst, volgens het zogenaamde software-als-een-dienst (Software-as-a-Service, of afgekort, SaaS) business model. Ook financiële dienstverleners zijn SaaS aan het invoeren en passen hierdoor de wijze waarop zij hun diensten leveren en verlenen aan klanten aan.
openaire   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Spatio-Temporal Geographic Networks for Value Co-Creation and Technology Transfer in China with Patent Data. [PDF]

Sci Data
Su Y, Lv A, Zhi P, Chai J, Tian J.
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

How external stimuli drive physicians' value co-creation behavior in online health communities: the mediating role of risk-benefit perceptions and moderating role of digital competency. [PDF]

Front Public Health
Yao Y, Cao J, Wang J, Zhong Y, Feng M.
europepmc   +1 more source