Preliminary crystallographic analysis of recombinant VapBC-15 toxin-antitoxin complex from Mycobacterium tuberculosis. [PDF]
Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2013 The Mycobacterium tuberculosis vapBC15 locus encodes a toxin-antitoxin complex. VapC-15 is a toxin and possesses ribonuclease activity and VapB-15 is an antitoxin which both binds and inhibits the VapC-15 toxin. In this study, vapBC15 genes were cloned and co-expressed in Escherichia coli.
Uddipan Das +3 more
semanticscholar +5 more sources
Role of vapBC toxin-antitoxin loci in the thermal stress response of Sulfolobus solfataricus.
Biochemical Society Transactions, 2009 TA (toxin–antitoxin) loci are ubiquitous in prokaryotic micro-organisms, including archaea, yet their physiological function is largely unknown. For example, preliminary reports have suggested that TA loci are microbial stress-response elements, although it was recently shown that knocking out all known chromosomally located TA loci in Escherichia coli
C. Cooper +4 more
semanticscholar +5 more sources
The crystal structure of the Rv0301‐Rv0300 VapBC‐3 toxin—antitoxin complex from M. tuberculosis reveals a Mg2+ ion in the active site and a putative RNA‐binding site [PDF]
Protein Science, 2012 AbstractVapBC pairs account for 45 out of 88 identified toxin‐antitoxin (TA) pairs in the Mycobacterium tuberculosis (Mtb) H37Rv genome. A working model suggests that under times of stress, antitoxin molecules are degraded, releasing the toxins to slow the metabolism of the cell, which in the case of VapC toxins is via their RNase activity.
A. Min +5 more
semanticscholar +4 more sources
VapC toxins from Mycobacterium tuberculosis are ribonucleases that differentially inhibit growth and are neutralized by cognate VapB antitoxins. [PDF]
PLoS ONE, 2011 The chromosome of Mycobacterium tuberculosis (Mtb) encodes forty seven toxin-antitoxin modules belonging to the VapBC family. The role of these modules in the physiology of Mtb and the function(s) served by their expansion are unknown.
Bintou Ahmadou Ahidjo +7 more
doaj +13 more sources
Impact of Genomic Deletion RD16 on the Expression of the Mycobacterium bovis BCG Moreau VapBC47 Toxin-Antitoxin System [PDF]
Current Issues in Molecular Biology, 2023 Mycobacterium bovis BCG is the only vaccine against tuberculosis. The variable forms of cultivation throughout the years, before seed-lots were developed, allowed in vitro evolution of the original strain, generating a family of vaccines with different ...
Talita Duarte Pagani +8 more
doaj +2 more sources
A VapBC Toxin-Antitoxin Module Is a Posttranscriptional Regulator of Metabolic Flux in Mycobacteria [PDF]
Journal of Bacteriology, 2012 ABSTRACT The largest family of toxin-antitoxin (TA) modules are encoded by the vapBC operons, but their roles in bacterial physiology remain enigmatic. Microarray analysis in Mycobacterium smegmatis overexpressing VapC/VapBC revealed a high percentage of downregulated genes with ...
J. McKenzie +7 more
semanticscholar +5 more sources
Mycobacterium tuberculosis Rv0229c Shows Ribonuclease Activity and Reveals Its Corresponding Role as Toxin VapC51 [PDF]
Antibiotics, 2023 The VapBC system, which belongs to the type II toxin–antitoxin (TA) system, is the most abundant and widely studied system in Mycobacterium tuberculosis.
Sung-Min Kang
doaj +2 more sources
The VapBC Toxin–Antitoxin System Enhances Shigella flexneri Fitness Through Coordination of Metabolic Stress Adaptation and Virulence [PDF]
bioRxivAbstractShigella flexneriis a facultative intracellular pathogen that causes bacillary dysentery by invading and replicating within intestinal epithelial cells. Successful intracellular survival requires the bacterium to balance metabolic adaptation with the sustained expression of virulence programs by the type III secretion system (T3SS).
Estelle Saifi +8 more
semanticscholar +3 more sources
VapC toxins promote the pathogenesis of Rickettsia heilongjiangensis by cleaving essential RNAs from both Rickettsia and its host. [PDF]
PLoS PathogensToxin-antitoxin (TA) modules enable bacteria to persist under stressful environments. However, they are typically absent from host-associated prokaryotes due to their potential host toxicity. Here, the obligate intracellular bacterium spotted fever group
Yan Liu +10 more
doaj +2 more sources
Clonagem, expressão e caracterização inicial do módulo VapBC-4 de >i/i [PDF]
, 2020 Bruna Oliveira Pigatto Azevedo
semanticscholar +3 more sources