Results 41 to 50 of about 756 (133)

Prokaryote toxin–antitoxin modules: Complex regulation of an unclear function

Protein Science, Volume 30, Issue 6, Page 1103-1113, June 2021., 2021
Abstract Toxin–antitoxin (TA) modules are small operons in bacteria and archaea that encode a metabolic inhibitor (toxin) and a matching regulatory protein (antitoxin). While their biochemical activities are often well defined, their biological functions remain unclear. In Type II TA modules, the most common class, both toxin and antitoxin are proteins,
Pieter De Bruyn, Yana Girardin, Remy Loris   +2 more
wiley   +1 more source

Structure‐based design of peptides that trigger Streptococcus pneumoniae cell death

The FEBS Journal, Volume 288, Issue 5, Page 1546-1564, March 2021., 2021
HigBA complex shows a crossed‐scissor interface with two intermolecular β‐sheets at both the N and C termini of the HigA antitoxin. The crossed‐scissor constitutes a new type of interface between HigB and HigA. We report a unique structure of the Streptococcus pneumoniae HigBA system and a novel antimicrobial agent that activates HigB toxin, which ...
Sung‐Min Kang, Chenglong Jin, Do‐Hee Kim, Sung Jean Park, Sang‐Woo Han, Bong‐Jin Lee   +5 more
wiley   +1 more source

Isolation of persister cells within the biofilm and relative gene expression analysis of type II toxin/antitoxin system in Pseudomonas aeruginosa isolates in exponential and stationary phases

Journal of Global Antimicrobial Resistance, 2022
: Objectives: Chronic infections and treatment failure are concerning issues in patients with Pseudomonas aeruginosa infections. Persister cell formation in biofilm is considered a key reason for antibiotic resistance and treatment failure.
Rezvan Golmoradi Zadeh, Behrooz Sadeghi Kalani, Marzie Mahdizade Ari, Malihe Talebi, Shabnam Razavi, Faramarz Masjedian Jazi   +5 more
doaj   +1 more source

Toxin-Activating Stapled Peptides Discovered by Structural Analysis Were Identified as New Therapeutic Candidates That Trigger Antibacterial Activity against Mycobacterium tuberculosis in the Mycobacterium smegmatis Model

Microorganisms, 2021
The structure-function relationships of toxin-antitoxin (TA) systems from Mycobacterium tuberculosis have prompted the development of novel and effective antimicrobial agents that selectively target this organism.
Sung-Min Kang, Heejo Moon, Sang-Woo Han, Byeong Wook Kim, Do-Hee Kim, Byeong Moon Kim, Bong-Jin Lee   +6 more
doaj   +1 more source

Analysis of non-typeable Haemophilous influenzae VapC1 mutations reveals structural features required for toxicity and flexibility in the active site. [PDF]

PLoS ONE, 2014
Bacteria have evolved mechanisms that allow them to survive in the face of a variety of stresses including nutrient deprivation, antibiotic challenge and engulfment by predator cells.
Brooke Hamilton, Alexander Manzella, Karyn Schmidt, Victoria DiMarco, J Scott Butler   +4 more
doaj   +1 more source

Preliminary crystallographic analysis of recombinant VapBC-15 toxin–antitoxin complex fromMycobacterium tuberculosis [PDF]

Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2013
The Mycobacterium tuberculosis vapBC15 locus encodes a toxin-antitoxin complex. VapC-15 is a toxin and possesses ribonuclease activity and VapB-15 is an antitoxin which both binds and inhibits the VapC-15 toxin. In this study, vapBC15 genes were cloned and co-expressed in Escherichia coli.
Uddipan, Das, Nitesh, Kumar, Samudrala, Gourinath, Alagiri, Srinivasan   +3 more
openaire   +2 more sources

Physiologic Stresses Reveal a Salmonella Persister State and TA Family Toxins Modulate Tolerance to These Stresses. [PDF]

PLoS ONE, 2015
Bacterial persister cells are considered a basis for chronic infections and relapse caused by bacterial pathogens. Persisters are phenotypic variants characterized by low metabolic activity and slow or no replication.
Eugenia Silva-Herzog, Erin M McDonald, Amy L Crooks, Corrella S Detweiler   +3 more
doaj   +1 more source

Integrated mobile genetic elements in Thaumarchaeota

Environmental Microbiology, Volume 21, Issue 6, Page 2056-2078, June 2019., 2019
Summary To explore the diversity of mobile genetic elements (MGE) associated with archaea of the phylum Thaumarchaeota, we exploited the property of most MGE to integrate into the genomes of their hosts. Integrated MGE (iMGE) were identified in 20 thaumarchaeal genomes amounting to 2 Mbp of mobile thaumarchaeal DNA.
Mart Krupovic, Kira S. Makarova, Yuri I. Wolf, Sofia Medvedeva, David Prangishvili, Patrick Forterre, Eugene V. Koonin   +6 more
wiley   +1 more source

When Ribonucleases Come into Play in Pathogens: A Survey of Gram‐Positive Bacteria

International Journal of Microbiology, Volume 2012, Issue 1, 2012., 2012
It is widely acknowledged that RNA stability plays critical roles in bacterial adaptation and survival in different environments like those encountered when bacteria infect a host. Bacterial ribonucleases acting alone or in concert with regulatory RNAs or RNA binding proteins are the mediators of the regulatory outcome on RNA stability.
Brian C. Jester, Pascale Romby, Efthimia Lioliou, John Tagg   +3 more
wiley   +1 more source

The vapB-vapC operon of Acidovorax citrulli functions as a bona-fide toxin-antitoxin module

Frontiers in Microbiology, 2016
Toxin–antitoxin systems are commonly found on plasmids and chromosomes of bacteria and archaea. These systems appear as biscystronic genes encoding a stable toxin and a labile antitoxin, which protects the cells from the toxin’s activity. Under specific,
Reut eShavit, Mario eLebendiker, Zohar ePasternak, Saul eBurdman, Yael eHelman   +4 more
doaj   +1 more source