Results 61 to 70 of about 38,680 (247)

Vector Competence of Australian Mosquitoes for Yellow Fever Virus [PDF]

The American Society of Tropical Medicine and Hygiene, 2011
The vector competence of Australian mosquitoes for yellow fever virus (YFV) was evaluated. Infection and transmission rates in Cairns and Townsville populations of Aedes aegypti and a Brisbane strain of Ae.
van den Hurk, Andrew F., McElroy, Kate, Pyke, Alyssa T., McGee, Charles E., Hall-Mendelin, Sonja, Day, Andrew, Ryan, Peter A., Ritchie, Scott A., Vanlandingham, Dana L., Higgs, Stephen   +9 more
openaire   +5 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Secondary vectors of Zika Virus, a systematic review of laboratory vector competence studies

PLOS Neglected Tropical Diseases, 2023
Background After the unprecedented Zika virus (ZIKV) outbreak in the western hemisphere from 2015–2018, Aedes aegypti and Ae. albopictus are now well established primary and secondary ZIKV vectors, respectively. Consensus about identification and importance of other secondary ZIKV vectors remain. This systematic review aims to provide a list of vector
Marina Bisia, Carlos Alberto Montenegro-Quiñonez, Peter Dambach, Andreas Deckert, Olaf Horstick, Antonios Kolimenakis, Valérie R. Louis, Pablo Manrique‐Saide, Αntonios Michaelakis, Silvia Runge‐Ranzinger, Amy C. Morrison   +10 more
openaire   +5 more sources

Determining dengue infection risk in the Colombo district of Sri Lanka by inferencing the genetic parameters of Aedes mosquitoes

BMC Infectious Diseases
Background For decades, dengue has posed a significant threat as a viral infectious disease, affecting numerous human lives globally, particularly in tropical regions, yet no cure has been discovered.
Piyumi Chathurangika, Lakmini S. Premadasa, S. S. N. Perera, Kushani De Silva   +3 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Evaluating vector competence for Yellow fever in the Caribbean. [PDF]

Nat Commun
Abstract The mosquito-borne disease, Yellow fever (YF), has been largely controlled via mass delivery of an effective vaccine and mosquito control interventions. However, there are warning signs that YF is re-emerging in both Sub-Saharan Africa and South America.
Gabiane G, Bohers C, Mousson L, Obadia T, Dinglasan RR, Vazeille M, Dauga C, Viglietta M, Yébakima A, Vega-Rúa A, Gutiérrez Bugallo G, Gélvez Ramírez RM, Sonor F, Etienne M, Duclovel-Pame N, Blateau A, Smith-Ravin J, De Lamballerie X, Failloux AB.   +18 more
europepmc   +5 more sources

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Aedes aegypti vector competence studies: A review

Infection, Genetics and Evolution, 2019
Aedes aegypti is the primary transmitter of the four viruses that have had the greatest impact on human health, the viruses causing yellow fever, dengue fever, chikungunya, and Zika fever. Because this mosquito is easy to rear in the laboratory and these viruses grow in laboratory tissue culture cells, many studies have been performed testing the ...
Jayme A. Souza-Neto, Jeffrey R. Powell, Mariangela Bonizzoni   +2 more
openaire   +4 more sources