Fluorescence Resonance Energy Transfer Detection of Synaptophysin I and Vesicle-associated Membrane Protein 2 Interactions during Exocytosis from Single Live Synapses [PDF]
Molecular Biology of the Cell, 2002 To investigate the molecular interactions of synaptophysin I and vesicle-associated membrane protein 2 (VAMP2)/synaptobrevin II during exocytosis, we have used time-lapse videomicroscopy to measure fluorescence resonance energy transfer in live neurons.
PENNUTO M +4 more
openaire +4 more sources
Membrane alterations induced by nonstructural proteins of human norovirus. [PDF]
PLoS Pathogens, 2017 Human noroviruses (huNoV) are the most frequent cause of non-bacterial acute gastroenteritis worldwide, particularly genogroup II genotype 4 (GII.4) variants.
Sylvie Y Doerflinger +10 more
doaj +1 more source
Human VAMP3 Suppresses or Negatively Regulates Bax Induced Apoptosis in Yeast
Biomedicines, 2021 Apoptosis is an essential process that is regulated genetically and could lead to a serious disease condition if not well controlled. Bax is one of the main proapoptotic proteins and actively involved in programmed cell death.
Damilare D. Akintade +1 more
doaj +1 more source
Localization and Function of SolubleN-Ethylmaleimide-sensitive Factor Attachment Protein-25 and Vesicle-associated Membrane Protein-2 in Functioning Gastric Parietal Cells [PDF]
Journal of Biological Chemistry, 2002 The soluble N-ethylmaleimide-sensitive factor attachment protein of 25 kDa (SNAP-25) plays an important role in vesicle trafficking. Together with vesicle-associated membrane protein-2 (VAMP-2) and syntaxin, SNAP-25 forms a ternary complex implicated in docking and fusion of secretory vesicles with the plasma membrane during exocytosis. These so-called
Serhan, Karvar +4 more
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Structural Disorder Provides Increased Adaptability for Vesicle Trafficking Pathways
, 2013 Vesicle trafficking systems play essential roles in the communication between the organelles of eukaryotic cells and also between cells and their environment.
Peter Tompa +11 more
core +1 more source
Atlas of interactions between SARS-CoV-2 macromolecules and host proteins
Cell Insight, 2023 The proteins and RNAs of viruses extensively interact with host proteins after infection. We collected and reanalyzed all available datasets of protein-protein and RNA-protein interactions related to SARS-CoV-2.
Guangnan Li +8 more
doaj +1 more source
Exploring the functional domain and the target of the tetanus toxin light chain in neurohypophysial terminals [PDF]
, 1994 The tetanus toxin light chain blocks calcium induced vasopressin release from neurohypophysial nerve terminals. Here we show that histidine residue 233 within the putative zinc binding motif of the tetanus toxin light chain is essential for the ...
Weller, U. +7 more
core +1 more source
Dense core secretory vesicles revealed as a dynamic Ca2+store in neuroendocrine cells with a vesicle-associated membrane protein aequorin chimaera [PDF]
The Journal of Cell Biology, 2001 The role of dense core secretory vesicles in the control of cytosolic-free Ca(2+) concentrations ([Ca(2+)](c)) in neuronal and neuroendocrine cells is enigmatic. By constructing a vesicle-associated membrane protein 2-synaptobrevin.aequorin chimera, we show that in clonal pancreatic islet beta-cells: (a) increases in [Ca(2+)](c) cause a prompt increase
MITCHELL KJ +7 more
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FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
The FASEB Journal, 2014 Soluble NSF Attachment Protein Receptors (SNAREs) drive membrane fusion in eukaryotic cells. SNAREs of the Vesicle Associated Membrane Protein (VAMP) family reside in vesicles and mediate vesicle fusion. After fusion, VAMP remains inserted in the plasma membrane until retrieved for another round of fusion.
Paulo Caceres, Pablo Ortiz
openaire +1 more source