Results 71 to 80 of about 24,194,770 (306)

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Kissing interaction between 3' noncoding and coding sequences is essential for porcine arterivirus RNA replication [PDF]

, 2002
We used an infectious cDNA clone of Porcine reproductive and respiratory syndrome virus (PRRSV) to investigate the presence of essential replication elements in the region of the genome encoding the structural proteins.
R. C. L. Olsthoorn, Rottier, P.J.M., J. J. M. Meulenberg, Olsthoorn, R.R.C.L., M. H. Verheije, P. J. M. Rottier, M. V. Kroese, Meulenberg, J.J.M., Verheije, M.H., Olsthoorn, R.C.L., Kroese, M.V.   +10 more
core   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis [PDF]

mBio, 2015
ABSTRACT  Insensitivity and technical complexity have impeded the implementation of high-throughput nucleic acid sequencing in differential diagnosis of viral infections in clinical laboratories. Here, we describe the development of a virome capture sequencing platform for vertebrate viruses (VirCapSeq-VERT) that increases the sensitivity of ...
Briese, Thomas, Kapoor, Amit, Mishra, Nischay, Jain, Komal, Kumar, Arvind, Jabado, Omar J., Lipkin, W. Ian, Moscona, Anne   +7 more
openaire   +4 more sources

Generation and characterization of a recombinant Rift Valley fever virus expressing a V5 epitope-tagged RNA-dependent RNA polymerase [PDF]

, 2011
The viral RNA-dependent RNA polymerase (RdRp; L protein) of Rift Valley fever virus (RVFV; family Bunyaviridae) is a 238 kDa protein that is crucial for the life cycle of the virus, as it catalyses both transcription of viral mRNAs and replication of the
Brennan, Benjamin, Richard M. Elliott, Li, Ping, Ping Li, Elliott, Richard M., Benjamin Brennan   +5 more
core   +1 more source

Reconstructing Prehistoric Viral Genomes from Neanderthal Sequencing Data

Viruses
DNA viruses that produce persistent infections have been proposed as potential causes for the extinction of Neanderthals, and, therefore, the identification of viral genome remnants in Neanderthal sequence reads is an initial step to address this ...
Renata C. Ferreira, Gustavo V. Alves, Marcello Ramon, Fernando Antoneli, Marcelo R. S. Briones   +4 more
doaj   +1 more source

VADR: validation and annotation of virus sequence submissions to GenBank

BMC Bioinformatics, 2020
Background GenBank contains over 3 million viral sequences. The National Center for Biotechnology Information (NCBI) previously made available a tool for validating and annotating influenza virus sequences that is used to check submissions to GenBank ...
Alejandro A. Schäffer, Eneida L. Hatcher, Linda Yankie, Lara Shonkwiler, J. Rodney Brister, Ilene Karsch-Mizrachi, Eric P. Nawrocki   +6 more
doaj   +1 more source

Comparative analysis of seven viral nuclear export signals (NESs) reveals the crucial role of nuclear export mediated by the third NES consensus sequence of nucleoprotein (NP) in influenza A virus replication. [PDF]

PLoS ONE, 2014
The assembly of influenza virus progeny virions requires machinery that exports viral genomic ribonucleoproteins from the cell nucleus. Currently, seven nuclear export signal (NES) consensus sequences have been identified in different viral proteins ...
Nopporn Chutiwitoonchai, Michinori Kakisaka, Kazunori Yamada, Yoko Aida   +3 more
doaj   +1 more source

Epigenetic reprogramming of lineage switching in cancer

FEBS Letters, EarlyView.
Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı, Burcu Akman, Ezgi Bağırsakçı, Nese Atabey, Serap Erkek‐Ozhan   +4 more
wiley   +1 more source

The influence of viral RNA secondary structure on interactions with innate host cell defences [PDF]

, 2013
RNA viruses infecting vertebrates differ fundamentally in their ability to establish persistent infections with markedly different patterns of transmission, disease mechanisms and evolutionary relationships with their hosts.
Blundell, Richard, Richard Blundell, Nora McFadden, Joris J. Maarleveld, Maarleveld, Joris J, Evans, David J, Simmonds, Peter, Jeroen Witteveldt, Maarleveld, Joris J., David J. Evans, McFadden, Nora, Peter Simmonds, Evans, David J., Witteveldt, Jeroen   +13 more
core   +1 more source