Results 71 to 80 of about 824,852 (209)

Viral vectors : gene therapy and neuroscience applications /

, 1995
Genetic manipulation of the adult mammalian nervous system is one of the most exciting areas in contemporary neurobiology. The explosive growth of this field has been facilitated by harnessing the power of viruses to transfer genetic material into ...
Loewy, Arthur D., Kaplitt, Michael G.
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

In situ transduction of target cells on solid surfaces by immobilized viral vectors

BMC Biotechnology, 2003
Background For both in vitro and in vivo gene transfer applications, recombinant viral vectors have almost always been used free in solution. Some site-specificity of the delivery of viral vectors can be achieved by applying a solution containing viral ...
Pandori Mark W, Hobson David A, Sano Takeshi   +2 more
doaj   +1 more source

Adeno-Associated Viral Vectors in Neuroscience Research

Molecular Therapy: Methods & Clinical Development, 2020
Adeno-associated viral vectors (AAVs) are increasingly useful preclinical tools in neuroscience research studies for interrogating cellular and neurocircuit functions and mapping brain connectivity.
David L. Haggerty, Gregory G. Grecco, Kaitlin C. Reeves, Brady Atwood   +3 more
doaj   +1 more source

Overview of current scalable methods for purification of viral vectors

, 2011
As a result of the growing interest in the use of viruses for gene therapy and vaccines, many virus-based products are being developed. The manufacturing of viruses poses new challenges for process developers and regulating authorities that need to be ...
Garnier, Alain, Segura, Maria Mercedes, María Mercedes Segura, Kamen, Amine A., Amine A. Kamen, Alain Garnier   +5 more
core   +1 more source

Constrained pattern of viral evolution in acute and early HCV infection limits viral plasticity [PDF]

, 2011
Cellular immune responses during acute Hepatitis C virus (HCV) and HIV infection are a known correlate of infection outcome. Viral adaptation to these responses via mutation(s) within CD8+ T-cell epitopes allows these viruses to subvert host immune ...
Paul Klenerman, Malte Heeg, John, Mina, Pfafferott, K, James, Ian, Ulsenheimer, Axel, Helmut M. Diepolder, Lucas, A.D., Silvana Gaudieri, Heeg, M, Rauch, A, Ian James, John, M., Andri Rauch, Klenerman, P., James, I, Simon Mallal, Diepolder, HM, Heeg, Malte, John, M, Mallal, Simon, Lucas, Andrew, Nolan, D., Lucas, M., Heeg, M., Michaela Lucas, Diepolder, H.M., Mallal, S., Gaudieri, S, Lucas, M, Gaudieri, S., Nolan, David, Ulsenheimer, A, Lucas, A, David Nolan, Klenerman, Paul, Diepolder, Helmut M., Pfafferott, K., Klenerman, P, Rauch, A., Ulsenheimer, A., Pfafferott, Katja, Lucas, Michaela, Katja Pfafferott, Andrew Lucas, Rauch, Andri, Diepolder, Helmut M, Axel Ulsenheimer, Mina John, Helmut M Diepolder, Nolan, D, Mallal, S, James, I., Gaudieri, Silvana   +53 more
core   +3 more sources

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

The next-generation DNA vaccine platforms and delivery systems: advances, challenges and prospects

Frontiers in Immunology
Vaccines have proven effective in the treatment and prevention of numerous diseases. However, traditional attenuated and inactivated vaccines suffer from certain drawbacks such as complex preparation, limited efficacy, potential risks and others.
Bowen Lu, Bowen Lu, Bowen Lu, Jing Ming Lim, Boyue Yu, Siyuan Song, Praveen Neeli, Navid Sobhani, Pavithra K, Srinivasa Reddy Bonam, Rajendra Kurapati, Junnian Zheng, Junnian Zheng, Dafei Chai, Dafei Chai, Dafei Chai   +15 more
doaj   +1 more source

Complete, long-lasting protection against malaria of mice primed and boosted with two distinct viral vectors expressing the same plasmodial antigen. [PDF]

, 2001
We report that complete protection against malaria and total inhibition of liver stage development and parasitemia was obtained in 100% of BALB/c mice primed with a replication-defective recombinant adenovirus expressing the circumsporozoite (CS) protein
Oscar Bruña-Romero, Gloria González-Aseguinolaza, Ruth S. Nussenzweig, Tsuji, M, González-Aseguinolaza, G, Julius C. R. Hafalla, Bruña-Romero, O, Hafalla, JC, Nussenzweig, RS, Moriya Tsuji   +9 more
core   +1 more source