Results 91 to 100 of about 722,631 (394)

Development of Virus-Like-Particle Vaccine and Reporter Assay for Zika Virus

Journal of Virology, 2017
Recent worldwide outbreaks of Zika virus (ZIKV) infection and the lack of an approved vaccine raise serious concerns regarding preparedness to combat this emerging virus.
H. Garg, Melina J. Sedano, Gabrielle Plata, E. Punke, A. Joshi   +4 more
semanticscholar   +1 more source

Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells

Molecular Oncology, EarlyView.
Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, Gökhan Karakülah, Serif Senturk   +11 more
wiley   +1 more source

Universal Particle Kinetic Distribution in Crowded Environments [PDF]

, 2020
We study many-particle transport in heterogeneous, crowded environments at different particle P\'{e}clet numbers ($Pe^*$). We demonstrate that a modified Nakagami-$m$ function describes particle velocity probability distributions when particle deposition occurs.
arxiv   +1 more source

Virus-like Particle Display of the α-Gal Carbohydrate for Vaccination against Leishmania Infection

ACS Central Science, 2017
Secreted and surface-displayed carbohydrates are essential for virulence and viability of many parasites, including for immune system evasion. We have identified the α-Gal trisaccharide epitope on the surface of the protozoan parasites Leishmania ...
A. P. Moura, Luiza C. B. Santos, C. R. N. Brito, E. Valencia, Caroline Junqueira, A. A. P. Filho, M. R. Sant'Anna, N. F. Gontijo, D. Bartholomeu, R. Fujiwara, R. Gazzinelli, Craig S Mckay, Carlos A. Sanhueza, M. G. Finn, A. Marques   +14 more
semanticscholar   +1 more source

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays

FEBS Open Bio, EarlyView.
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques, Edit Ábrahám, Hiromi Muramatsu, Daniel Youssef Bargieri, Norbert Pardi, Zoltán Lipinszki   +5 more
wiley   +1 more source

Polymer Structure and Conformation Alter the Antigenicity of Virus-like Particle-Polymer Conjugates.

Journal of the American Chemical Society, 2017
Covalent conjugation of water-soluble polymers to proteins is critical for evading immune surveillance in the field of biopharmaceuticals. The most common and long-standing polymer modification is the attachment of methoxypoly(ethylene glycol) (mPEG ...
Parker W Lee, Sergey A. Isarov, Jaqueline D. Wallat, S. Molugu, Sourabh Shukla, Jessie E. P. Sun, Jun Zhang, Yi Zheng, Melissa Lucius Dougherty, Dominik Konkolewicz, P. Stewart, N. Steinmetz, Michael J. A. Hore, J. Pokorski   +13 more
semanticscholar   +1 more source

Virus-like particles in vaccine development

Expert Review of Vaccines, 2010
Virus-like particles (VLPs) are multiprotein structures that mimic the organization and conformation of authentic native viruses but lack the viral genome, potentially yielding safer and cheaper vaccine candidates. A handful of prophylactic VLP-based vaccines is currently commercialized worldwide: GlaxoSmithKline's Engerix (hepatitis B virus) and ...
Leda R. Castilho, Paula M. Alves, António Roldão, Maria Candida M. Mellado, Manuel J.T. Carrondo   +4 more
openaire   +3 more sources

Mapping Hsp104 interactions using cross‐linking mass spectrometry

FEBS Open Bio, EarlyView.
This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal, Karolina Michalska, Andrzej Joachimiak, Lukasz A. Joachimiak   +3 more
wiley   +1 more source

Marburg virus-like particles by co-expression of glycoprotein and matrix protein in insect cells induces immune responses in mice

Virology Journal, 2017
Background Marburg virus (MARV) causes severe haemorrhagic fever in humans and nonhuman primates and has a high mortality rate. However, effective drugs or licensed vaccines are not currently available to control the outbreak and spread of this disease ...
Weiwei Gai, Xuexing Zheng, Chong Wang, Hualei Wang, Yongkun Zhao, Qi Wang, Gary Wong, Weijiao Zhang, Na Feng, Boning Qiu, Hang Chi, Nan Li, Tiecheng Wang, Yuwei Gao, Junjie Shan, Songtao Yang, Xianzhu Xia   +16 more
doaj   +1 more source

Virus-like Particles for TEM Regulation and Antitumor Therapy

Journal of Functional Biomaterials, 2022
Tumor development and metastasis are intimately associated with the tumor microenvironment (TME), and it is difficult for vector-restricted drugs to act on the TME for long-term cancer immunotherapy.
Zhu Yang, Yongjie Chi, Jiaxin Bao, Xin Zhao, Jing Zhang, Lianyan Wang   +5 more
doaj   +1 more source