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siRNA Screen of Early Poxvirus Genes Identifies the AAA+ ATPase D5 as the Virus Genome-Uncoating Factor [PDF]
SummaryPoxvirus genome uncoating is a two-step process. First, cytoplasmic viral cores are activated and early viral genes are expressed. Next, cores are disassembled and the genomes released. This second step depends on an early viral factor(s) that has
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Unanchored ubiquitin in virus uncoating
Science, 2014Components of a cellular degradation system are exploited by influenza virus during infection [Also see Report by Banerjee et al. ]
Ricardo Rajsbaum, Adolfo García-Sastre
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Uncoating of Tobacco Mosaic Virus RNA in Protoplasts
Virology, 1994The disassembly of tobacco mosaic virus particles during the establishment of infections in tobacco protoplasts was investigated by reverse transcription and PCR analysis of the parts of the viral RNA that remained encapsidated at various times after inoculation. Within the first 3 min, uncoating had proceeded from the 5' terminus of the viral RNA to a
X, Wu, Z, Xu, J G, Shaw
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Characterization of intermediates in the uncoating of vaccinia virus DNA
Virology, 1972Abstract The fate of parental vaccinia virions in mouse L fibroblasts was studied. Four particulate subviral intermediates of uncoating were detected and were partially characterized. SVC (subviral component) 1 had the “mulberry” appearance of virions and possessed the same polypeptide complement, but sedimented slightly slower.
I, Sarov, W K, Joklik
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Plant virus uncoating as a result of virus-cell wall interactions
Virology, 1979The fate of tobacco rattle virus (TRV) particles was studied after leaf panel infiltration. It was found that end-on virus attachment to cell walls of hosts (Nicotiana tobacum L. var. Xanthi-nc) and nonhosts (Zea mays L.) occurred, and that a virus degradative phase commenced immediately after attachment.
G, Gaard, G A, de Zoeten
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Journal of Virology, 1985
Input vaccinia virus deoxyribonucleoproteids with buoyant densities (in CsCl) very similar (if not identical) to those of viral cores have been found in large cytoplasmic structures in which viral DNA replication takes place. The deoxyribonucleoproteids consist of at least five major and two minor core proteins and viral DNA which is protected against ...
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Input vaccinia virus deoxyribonucleoproteids with buoyant densities (in CsCl) very similar (if not identical) to those of viral cores have been found in large cytoplasmic structures in which viral DNA replication takes place. The deoxyribonucleoproteids consist of at least five major and two minor core proteins and viral DNA which is protected against ...
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Uncoating of a Rimantadine-resistant Variant of Influenza Virus in the Presence of Rimantadine
A rimantadine-resistant variant of the Texas strain of influenza virus (Tr) was obtained by serial passages in eggs in MDCK cells in the presence of the drug, and its uncoating in MDCK cells was compared to that of the sensitive variant (Ts). First and second steps of uncoating were defined respectively by the appearance of subviral particles (SVP) in ...
A G Bukrinskaya
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The Assay, Purification and Properties of Vaccinia Virus-induced Uncoating Protein
Vaccinia virus cores prepared in vitro can be uncoated by incubation with extracts of cells infected with vaccinia virus, as determined by the conversion of the genome to DNase susceptibility. This uncoating activity had all the characteristics of the corresponding in vivo activity and of the agent responsible for non-genetic reactivation. Thus, it was
C B, Pedley, R J, Cooper
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The uncoating of alfalfa mosaic virus by its own RNA
Virology, 1972Abstract Upon addition of alfalfa mosaic virus (AMV)-RNA to AMV particles the latter are rapidly changed into highly RNase-sensitive, slow-sedimenting structures. These structures still contain intact RNA but they band at a higher density in CsCl gradients than virus particles and lack the typical bacilliform virus structure.
L, Van Vloten-Doting, E M, Jaspars
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Uncoating of turnip yellow mosaic virus RNA in vivo
Virology, 1985Following mechanical inoculation of leaves with turnip yellow mosaic virus (TYMV), a significant proportion of the retained inoculum is uncoated within 45 sec, and the process is more or less complete after 2 min. At least 80-90% of the uncoating takes place in the epidermis.
R E, Matthews, J, Witz
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