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siRNA Screen of Early Poxvirus Genes Identifies the AAA+ ATPase D5 as the Virus Genome-Uncoating Factor [PDF]

open access: yesCell Host and Microbe, 2014
SummaryPoxvirus genome uncoating is a two-step process. First, cytoplasmic viral cores are activated and early viral genes are expressed. Next, cores are disassembled and the genomes released. This second step depends on an early viral factor(s) that has
Samuel Kilcher   +2 more
exaly   +2 more sources
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Unanchored ubiquitin in virus uncoating

Science, 2014
Components of a cellular degradation system are exploited by influenza virus during infection [Also see Report by Banerjee et al. ]
Ricardo Rajsbaum, Adolfo García-Sastre
openaire   +1 more source

Uncoating of Tobacco Mosaic Virus RNA in Protoplasts

Virology, 1994
The disassembly of tobacco mosaic virus particles during the establishment of infections in tobacco protoplasts was investigated by reverse transcription and PCR analysis of the parts of the viral RNA that remained encapsidated at various times after inoculation. Within the first 3 min, uncoating had proceeded from the 5' terminus of the viral RNA to a
X, Wu, Z, Xu, J G, Shaw
openaire   +2 more sources

Characterization of intermediates in the uncoating of vaccinia virus DNA

Virology, 1972
Abstract The fate of parental vaccinia virions in mouse L fibroblasts was studied. Four particulate subviral intermediates of uncoating were detected and were partially characterized. SVC (subviral component) 1 had the “mulberry” appearance of virions and possessed the same polypeptide complement, but sedimented slightly slower.
I, Sarov, W K, Joklik
openaire   +2 more sources

Plant virus uncoating as a result of virus-cell wall interactions

Virology, 1979
The fate of tobacco rattle virus (TRV) particles was studied after leaf panel infiltration. It was found that end-on virus attachment to cell walls of hosts (Nicotiana tobacum L. var. Xanthi-nc) and nonhosts (Zea mays L.) occurred, and that a virus degradative phase commenced immediately after attachment.
G, Gaard, G A, de Zoeten
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Uncoating of vaccinia virus

Journal of Virology, 1985
Input vaccinia virus deoxyribonucleoproteids with buoyant densities (in CsCl) very similar (if not identical) to those of viral cores have been found in large cytoplasmic structures in which viral DNA replication takes place. The deoxyribonucleoproteids consist of at least five major and two minor core proteins and viral DNA which is protected against ...
openaire   +2 more sources

Uncoating of a Rimantadine-resistant Variant of Influenza Virus in the Presence of Rimantadine

open access: yesJournal of General Virology, 1982
A rimantadine-resistant variant of the Texas strain of influenza virus (Tr) was obtained by serial passages in eggs in MDCK cells in the presence of the drug, and its uncoating in MDCK cells was compared to that of the sensitive variant (Ts). First and second steps of uncoating were defined respectively by the appearance of subviral particles (SVP) in ...
A G Bukrinskaya
exaly   +3 more sources

The Assay, Purification and Properties of Vaccinia Virus-induced Uncoating Protein

open access: yesJournal of General Virology, 1987
Vaccinia virus cores prepared in vitro can be uncoated by incubation with extracts of cells infected with vaccinia virus, as determined by the conversion of the genome to DNase susceptibility. This uncoating activity had all the characteristics of the corresponding in vivo activity and of the agent responsible for non-genetic reactivation. Thus, it was
C B, Pedley, R J, Cooper
exaly   +3 more sources

The uncoating of alfalfa mosaic virus by its own RNA

Virology, 1972
Abstract Upon addition of alfalfa mosaic virus (AMV)-RNA to AMV particles the latter are rapidly changed into highly RNase-sensitive, slow-sedimenting structures. These structures still contain intact RNA but they band at a higher density in CsCl gradients than virus particles and lack the typical bacilliform virus structure.
L, Van Vloten-Doting, E M, Jaspars
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Uncoating of turnip yellow mosaic virus RNA in vivo

Virology, 1985
Following mechanical inoculation of leaves with turnip yellow mosaic virus (TYMV), a significant proportion of the retained inoculum is uncoated within 45 sec, and the process is more or less complete after 2 min. At least 80-90% of the uncoating takes place in the epidermis.
R E, Matthews, J, Witz
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