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Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification
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Vitrification of organized tissues
Cryobiology, 1989Why vitrify tissues? The storage of viable mammalian tissues in the vitreous rather than the frozen state presents its own range of complex problems. These include cellular tolerance of highly concentrated cryoprotectant solutions, cooling and warming tissues at relatively high rates, thermal shock, cracking in aqueous glasses, and devitrification (i.e.
W J, Armitage, S J, Rich
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Vitrification of human monocytes
Cryobiology, 1986Human monocytes purified from peripheral blood by counterflow centrifugal elutriation were cryopreserved in a vitreous state at 1 atm pressure. The vitrification solution was Hanks' balanced salt solution (HBSS) containing (w/v) 20.5% Me2SO, 15.5% acetamide, 10% propylene glycol, and 6% polyethylene glycol.
T, Takahashi +5 more
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Vitrification of human oocytes
Minerva Obstetrics and Gynecology, 2018In recent years, growing evidence for the safety and efficiency of oocyte vitrification has made this technique be increasingly proposed for fertility preservation (FP). The populations who could benefit from FP include oncological patients who need the option to preserve their gametes before undergoing potential sterilizing treatment, patients with ...
Aila, Coello +2 more
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Thermodynamic aspects of vitrification
Cryobiology, 2010Vitrification is a process in which a liquid begins to behave as a solid during cooling without any substantial change in molecular arrangement or thermodynamic state variables. The physical phenomenon of vitrification is relevant to both cryopreservation by freezing, in which cells survive in glass between ice crystals, and cryopreservation by ...
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Cell encapsulating droplet vitrification
Lab on a Chip, 2007The capability to encapsulate single cells in droplets while retaining high cell viability (>90%) has great impact on tissue engineering, high-throughput screening, as well as clinical diagnostics and therapeutics. We demonstrate a novel method to vitrify a small number of cells using cell-encapsulating droplets.
Utkan, Demirci, Grace, Montesano
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Human Blastocyst Biopsy and Vitrification
Journal of Visualized Experiments, 2019Blastocyst biopsy is performed to obtain a reliable genetic diagnosis during IVF cycles with preimplantation genetic testing. Then, the ideal workflow entails a safe and efficient vitrification protocol, due to the turnaround time of the diagnostic techniques and to transfer the selected embryo(s) on a physiological endometrium in a following natural ...
Maggiulli R. +4 more
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72. Cryopreservation of Passiflora pohlii – Vitrification and encapsulation-vitrification
Cryobiology, 2012Passiflora pohlii is a wild tropical species of the genus Passiflora , it is native to Brazil and found in Cerrado, Atlantic Forest and Pantanal. This species has a potential agronomic interest due to its tolerance to soil-borne pathogens of Phytophthora sp, which causes damage to passionfruit culture. P.
T.S.M. Merhy +4 more
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