Vitrification, encapsulation-vitrification and droplet-vitrification: a review.
This paper discusses the importance of the successive steps of the vitrification technique and reviews the current development and use of vitrification and of the two derived protocols, encapsulation-vitrification and droplet-vitrification. Vitrification refers to the physical process by which a highly concentrated cryoprotective solution supercools to
Sakai, A., /Engelmann, Florent
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Research Progresses in Vitrification by Minimum Volume Method
Vitrification is an effective method in the field of cryopreservation since it can prevent the formation of ice crystals. Vitrification requires ultra-fast cooling rates and high concentrations of cryoprotective agents.
Zhang Xiaomin, Zhou Xinli
doaj +2 more sources
Background Vitrification has superseded the slow freezing method for cryopreservation of oocytes, embryos, and sperm, but there are as yet no standard protocols for its use in ovarian tissue cryopreservation (OTC). Published protocols diverge mainly with
J. Marschalek +6 more
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Vitrification Solutions for Plant Cryopreservation: Modification and Properties
Many plants cannot vitrify themselves because they lack glassy state-inducing substances and/or have high water content. Therefore, cryoprotectants are used to induce vitrification.
Jiri Zamecnik +2 more
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Development of alternative plant vitrification solutions in droplet-vitrification procedures.
This study aimed at developing alternative vitrification solutions, modified either from the original PVS2 vitrification solution by increasing glycerol and sucrose and/or decreasing dimethylsulfoxide and ethylene glycol concentration, or from the original PVS3 vitrification solution by decreasing glycerol and sucrose concentration.
Kim, H. H. +6 more
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Improving native human sperm freezing protection by using a modified vitrification method
Slow freezing is the most commonly used technique for the cryopreservation of spermatozoa in clinical practice. However, it has been shown to have a negative impact on sperm function and structure.
Dai Zhou +10 more
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Interfering Effects of In Vitro Fertilization and Vitrification on Expression of Gtl2 and Dlk1 in Mouse Blastocysts [PDF]
Background Embryo vitrification is a key instrument in assisted reproductive technologies (ARTs). However, there is increasing concern that vitrification adversely affects embryo development. This study intends to assess the effect of vitrification on
Elham Movahed +3 more
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Vitrification and Nanowarming of Kidneys [PDF]
AbstractVitrification can dramatically increase the storage of viable biomaterials in the cryogenic state for years. Unfortunately, vitrified systems ≥3 mL like large tissues and organs, cannot currently be rewarmed sufficiently rapidly or uniformly by convective approaches to avoid ice crystallization or cracking failures.
Anirudh Sharma +13 more
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Vitrification is essential for successful tissue cryopreservation and biobanking in wild cats. This study aimed to compare different methods of vitrification (Ovarian Tissue Cryosystem—OTC, Straws—STW, and Solid Surface vitrification—SSV) for testicular ...
Julyne Vivian Guimarães de Carvalho +6 more
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Overexpression of Mitochondrial Genes (Mitochondrial Transcription Factor A and Cytochrome c Oxidase Subunit 1) in Mouse Metaphase II Oocytes following Vitrification via Cryotop [PDF]
Background: Gamete cryopreservation is an inseparable part of assisted reproductive technology, and vitrification is an effective approach to the cryopreservation of oocytes.
Naeimeh Dehghani +4 more
doaj +1 more source

